Fatty acid uptake by Caco-2 human intestinal cells

Journal of Lipid Research

Volumn 37, Issue 2, 1996, Pages 336-346

  Trotter, Pamela J ^a,d   Ho, Shiu Ying ^b,c   Storch, Judith ^a,b,c  

^a HARVARD MEDICAL SCHOOL   (United States)

^b HARVARD SCHOOL OF PUBLIC HEALTH   (United States)

^c RUTGERS UNIVERSITY   (United States)

^d NATIONAL JEWISH MEDICAL AND RESEARCH CENTER   (United States)

Author keywords

Caco 2; cell polarity; intestinal epithelial cells; kinetics

Indexed keywords

FATTY ACID;

ARTICLE; BASOLATERAL MEMBRANE; COMPETITIVE INHIBITION; FATTY ACID TRANSPORT; HUMAN; HUMAN CELL; INTESTINE ABSORPTION; INTESTINE CELL; LIPID ANALYSIS; MEMBRANE TRANSPORT; PRIORITY JOURNAL;

ANIMALS; ANTIBODIES; BINDING, COMPETITIVE; BLOTTING, WESTERN; CACO-2 CELLS; CARRIER PROTEINS; EPITHELIUM; FATTY ACID-BINDING PROTEINS; FATTY ACIDS; HUMANS; INTESTINES; KINETICS; MYELIN P2 PROTEIN; NEOPLASM PROTEINS; NERVE TISSUE PROTEINS; OCTANOIC ACIDS; OLEIC ACID; PALMITIC ACID; PRONASE; RATS; TRYPSIN; TUMOR SUPPRESSOR PROTEINS;

EID: 13344276570     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (57)

1. Thomson, A. B. R., and J. M. Dietschy. 1981. Intestinal lipid absorption: major extracellular and intracellular events. In Physiology of the Gastrointestinal Tract. L. R. Johnson, editor. Raven Press, New York. 1147-1220.
2. Thomson, A. B. R., M. Keelan, M. L. Garg, and M. T. Clandinin. 1989. Intestinal aspects of lipid absorption: in review. Can. J. Physiol. Pharmacol. 67: 179-191.
3. Meddings, J. B. 1988. Lipid permeability of rat jejunum and ileum: correlation with physical properties of the microvillus membrane. Biochim. Biophys. Acta. 943: 305-314.
4. Kamp, F., and J. A. Hamilton. 1993. Movement of fatty acids, fatty acid analogues, and bile acids across phospholipid bilayers. Biochemistry. 32: 11074-11086.
5. Noy, N., T. M. Donnelly, and D. Zakim. 1986. Physical-chemical model for entry of water-insoluble compounds into cells. Studies of fatty acid uptake by the liver. Biochemistry. 25: 2013-2021.
6. Cooper, R. B., N. Noy, and D. Zakim. 1989. Mechanism for binding of fatty acids to hepatocyte plasma membranes. J. Lipid Res. 30: 1719-1726.
7. Westergaard, H., and J. M. Dietschy. 1974. Delineation of dimensions and permeability characteristics of the two major diffusion barriers to passive mucosal uptake in rabbit intestine. J. Clin. Invest. 54: 718-732.
8. Stremmel, W. 1989. Mechanism of hepatic fatty acid uptake. J. Hepatol. 9: 374-382.
9. Potter, B. J., D. Sorrentino, and P. D. Berk. 1989. Mechanisms of cellular uptake of free fatty acids. Annu. Rev. Nutr. 9: 253-270.
10. 14C]oleate reflects membrane transport rather than intracellular metabolism or binding. Proc. Natl. Acad. Sci. USA. 83: 3086-3090.
11. Abumrad, N. A., R. C. Perkins, J. H. Park, and C. R. Park. 1981. Mechanism of long chain fatty acid permeation in the isolated adipocyte. J. Biol. Chem. 256: 9183-9191.
12. Abumrad, N. A., J. H. Park, and C. R. Park. 1984. Permeation of long chain fatty acid into adipocytes. J. Biol. Chem. 259: 8945-8953.
13. Sorrentino, D., R. B. Robinson, C. L. Kiang, and P. D. Berk. 1989. At physiologic albumin/oleate concentrations oleate uptake by isolated hepatocytes, cardiac myocytes and adipocytes is a saturable function of the unbound oleate concentration. J. Clin. Invest. 84: 1325-1333.
14. Stremmel, W. 1988. Uptake of fatty acids by jejunal mucosal cells is mediated by a fatty acid binding membrane protein. J. Clin. Invest. 82: 2001-2010.
15. DeGrella, R. F., and R. J. Light. 1980. Uptake and metabolism of fatty acids by dispersed adult rat heart myocytes. II. Inhibition by albumin and fatty acid homologues and the effect of temperature and metabolic reagents. J. Biol. Chem. 255: 9739-9745.
16. Stremmel, W., G. Strohmeyer, and P. D. Berk. 1986. Hepatocellular uptake of oleate is energy dependent, sodium linked and inhibited by an antibody to a hepatocyte plasma membrane fatty acid binding protein. Proc. Natl. Acad. Sci. USA. 83: 3584-3588.
17. Stremmel, W., G. Lotz, G. Strohmeyer, and P. D. Berk. 1985. Identification, isolation, and partial characterization of a fatty acid binding protein from rat jejunal microvillus membranes. J. Clin. Invest. 75: 1068-1076.
18. Stremmel, W., G. Strohmeyer, F. Borchard, S. Kochwa, and P. D. Berk. 1985. Isolation and partial characterization of a fatty acid binding protein in rat liver plasma membranes. Proc. Natl. Acad. Sci. USA. 82: 4-8.
19. Schweiterman, W., D. Sorrentino, B. J. Potter, J. Rand, C. L. Kiang, D. Stump, and P. D. Berk. 1988. Uptake of oleate by isolated rat adipocytes is mediated by a 40-kDa plasma membrane fatty acid binding protein closely related to that in liver and gut. Proc. Natl. Acad. Sci. USA. 85: 359-363.
20. Fujii, S., H. Kawaguchi, and H. Yasuda. 1987. Purification of high affinity fatty acid receptors in rat myocardial sarcolemmal membranes. Lipids. 22: 544-546.
21. Stremmel, W., and L. Theilmann. 1986. Selective inhibition of long chain fatty acid uptake in short-term cultured rat hepatocytes by an antibody to rat liver plasma membrane fatty acid-binding protein. Biochim. Biophys. Acta. 877: 191-197.
22. Abumrad, N. A., M. R. El-Maghrabi, E-Z. Amri, E. Lopez, and P. A. Grimaldi. 1993. Cloning of a rat adipocyte membrane protein implicated in binding or transport of long-chain fatty acids that is induced during preadipocyte differentiation. J. Biol. Chem. 268: 17665-17668.
23. Schaffer, J. E., and H. F. Lodish. 1994. Expression cloning and characterization of a novel adipocyte long chain fatty acid transport protein. Cell. 79: 427-436.
24. Chow, S. L., and D. Hollander. 1979. A dual, concentration-dependent absorption mechanism of linoleic acid by rat jejunum in vitro. J. Lipid Res. 20: 349-356.
25. Ockner, R. K., J. P. Pittman, and J. L. Yager. 1972. Differences in the intestinal absorption of saturated and unsaturated long chain fatty acids. Gastroenterology. 62: 981-992.
26. Bennett Clark, S. 1971. The uptake of oleic acid by rat small intestine: a comparison of methodologies. J. Lipid Res. 12: 43-55.
27. Hollander, D., S. L. Chow, and V. D. Dedufalza. 1984. Intestinal absorption of free oleic acid in the unanesthetized rat: evidence for a saturable component? Can. J. Physiol. Pharmacol. 62: 1136-1140.
28. Goré, J., and C. Hoinard. 1993. Linolenic acid transport in hamster intestinal cells is carrier-mediated. J. Nutr. 123: 66-73.
29. Gangl, A., and R. K. Ockner. 1975. Intestinal metabolism of plasma free fatty acids. Intracellular compartmentation and mechanisms of control. J. Clin. Invest. 55: 803-813.
30. Trotter, P. J., and J. Storch. 1991. Fatty acid uptake and metabolism in a human intestinal cell line (Caco-2): comparison of apical and basolateral incubation. J. Lipid Res. 32: 293-304.
31. Levin, M. S., V. D. Talkad, J. I. Gordon, and W. F. Stenson. 1992. Trafficking of exogenous fatty acids within Caco-2 cells. J. Lipid Res. 33: 9-19.
32. Trotter, P. J., and J. Storch. 1993. Nutritional control of fatty acid esterification in differentiating Caco-2 intestinal cells is mediated by cellular diacylglycerol concentrations. J. Nutr. 123: 728-736.
33. Trotter, P. J., and J. Storch. 1993. Fatty acid esterification during differentiation of the human intestinal cell line Caco-2. J. Biol. Chem. 268: 10017-10023.
34. Spector, A. A., J. E. Fletcher, and J. D. Ashbrook. 1971. Analysis of long-chain free fatty acid binding to bovine serum albumin by determination of stepwise equilibrium constants. Biochemistry. 10: 3229-3232.
35. Spector, A. A. 1975. Fatty acid binding to plasma albumin. J. Lipid Res. 16: 165-179.
36. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with Folin phenol reagent. J. Biol. Chem. 193: 265-275.
37. Neame, K. D., and T. G. Richards. 1972. Elementary Kinetics of Membrane Carrier Transport. Blackwell Scientific Publications, Oxford, England.
38. Segal, I. H. 1975. Simple inhibition systems. In Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady State Enzyme Systems. John Wiley & Sons, New York. 100-125.
39. Small, D. M. 1986. The Physical Chemistry of Lipids: From Alkanes to Phospholipids. Plenum Press, New York. 276.
40. Hofmann, A. F., and H. S. Mekhjian. 1973. Bile acids and the intestinal absorption of fat and electrolytes in health and disease. In The Bile Acids, Vol. 2. P. P. Nair, and D. Kritchesvky, editors. Plenum Press, New York. 103-152.
41. Brasitus, T. A., and D. Schachter. 1980. Lipid dynamics and lipid-protein interactions in rat enterocyte basolateral and microvillus membranes. Biochemistry. 19: 2763-2769.
42. Jackson, M. J. 1974. Transport of short chain fatty acids. In Biomembranes. Vol. 4B. D. H. Smyth, editor. Plenum Press, London. 673-709.
43. Ranheim, T., A. Gedde-Dahl, A. C. Rustan, and C. A. Drevon. 1994. Fatty acid uptake and metabolism in CaCo-2 cells: eicosapentaenoic acid (20:5(n-3)) and oleic acid (18:1(n-9)) presented in association with micelles or albumin. Biochim. Biophys. Acta. 1212: 295-304.
44. Sallee, V. L. 1974. Apparent monomer activity of saturated fatty acids in micellar bile salt solutions measured by a polyethylene partitioning system. J. Lipid Res. 15: 56-64.
45. Sallee, V. L. 1978. Determinants of fatty acid and alcohol monomer activities in mixed micellar solutions. J. Lipid Res. 19: 207-214.
46. Proulx, P., H. Aubury, I. Brglez, and D. G. Williamson. 1984. Studies on the uptake of fatty acids by brush border membranes of rabbit intestine. Can. J. Biochem. Cell Biol. 63: 249-256.
47. Reed, R. G., and C. M. Burrington. 1989. The albumin receptor effect may be due to a surface-induced conformational change in albumin. J. Biol. Chem. 264: 9867-9872.
48. Shasby, D. M., L. L. Stoll, and A. A. Spector. 1987. Polarity of arachidonic acid metabolism by bovine aortic endothelial cell monolayers. Am. J. Physiol. 253: H1177-H1183.
49. Trimble, M. E. 1989. Mediated transport of long-chain fatty acids by rat renal basolateral membranes. Am. J. Physiol. 257: F539-F546.
50. Trimble, M. E. 1990. Renal palmitate transport: possible sites for interaction with a plasma membrane fatty acid-binding protein. Mol. Cell. Biochem. 98: 201-207.
51. Hansson, G. C. 1983. The subcellular localization of the glycosphingolipids in the epithelial cells of rat small intestine. Biochim. Biophys. Acta. 733: 295-299.
52. Van't Hof, W., and G. Van Meer. 1990. Generation of lipid polarity in intestinal epithelial (Caco-2) cells: sphingolipid synthesis in the Golgi complex and sorting before vesicular traffic to the plasma membrane. J. Cell Biol. 111: 977-986.
53. Schurer, N. Y., W. Stremmel, J. U. Grundmann, V. Schliep, H. Kleinert, N. M. Bass, and M. L. Williams. 1994. Evidence for a novel keratinocyte fatty acid uptake mechanism with preference for linoleic acid: comparison of oleic and linoleic acid uptake by cultured human keratinocytes, fibroblasts and a human hepatoma cell line. Biochim. Biophys. Acta. 1211: 51-60.
54. Ito, S. 1969. Structure and function of the glycocalyx. Fed. Proc. 28: 12-25.
55. Egberts, H. J. A., J. F. J. G. Koninkx, J. E. van Dijk, and J. M. V. M. Mouwen. 1984. Biological and pathobiological aspects of the glycocalyx of the small intestinal epithelium. A review. Vet. Q. 6: 186-199.
56. Ockner, R. K., and J. A. Manning. 1974. Fatty acid-binding protein in small intestine. Identification, isolation, and evidence for its role in cellular fatty acid transport. J. Clin. Invest. 54: 326-338.
57. Bass, N. M. 1985. Function and regulation of hepatic and intestinal fatty acid binding proteins. Chem. Phys. Lipids. 38: 95-114.