Improved isolation of genomic DNA from mycobacteria in agarose plugs by rapid lysis with a combination of N-acetylglucosaminidase and lysozyme

BioTechniques

Volumn 20, Issue 4, 1996, Pages 547-552

  Choe, Y K ^a   Huh, Y J ^b   Park, J H ^c   Kim, J R ^a   Park, J S ^d   Song, J C ^a   Ko, J H ^c   Lee, Y C ^c   Nashiru, O ^a   Kim, J K ^a   Kim, S J ^b   Bai, G H ^b   Chung, T H ^e   Chung, T W ^a   Choe, I S ^a   Kim, C H ^a  

^a Dongguk University   (South Korea)

^b Korean Institute of Tuberculosis   (South Korea)

^c Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^d Hannam University   (South Korea)

^e Kyungpook National University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLGLUCOSAMINIDASE; BACTERIAL DNA; LYSOZYME;

ARTICLE; BACTERIAL GENE; BACTERIUM ISOLATION; DIAGNOSTIC ACCURACY; DNA DETERMINATION; MYCOBACTERIUM AVIUM; MYCOBACTERIUM BOVIS; MYCOBACTERIUM PARATUBERCULOSIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PULSED FIELD GEL ELECTROPHORESIS; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

ACETYLGLUCOSAMINIDASE; BACTERIOLYSIS; BIOTECHNOLOGY; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA, BACTERIAL; ELECTROPHORESIS, GEL, PULSED-FIELD; HEAT; MURAMIDASE; MYCOBACTERIUM; MYCOBACTERIUM AVIUM; MYCOBACTERIUM BOVIS; MYCOBACTERIUM PARATUBERCULOSIS; MYCOBACTERIUM TUBERCULOSIS; SEPHAROSE; TIME FACTORS;

BACTERIA (MICROORGANISMS); CORYNEBACTERINEAE; MYCOBACTERIUM; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS; MYCOBACTERIUM BOVIS; MYCOBACTERIUM TUBERCULOSIS;

EID: 13344270897     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (8)

1. Bose, M., A. Chander and R.H. Das. 1993. A rapid and gentle method for the isolation of genomic DNA from mycobacteria. Nucleic Acids Res. 21:2529-2530.
2. Green, E.P., M.L.V. Tizard, M.T. Moss, J. Thompson, D.J. Winterbourne, J.J. McFadden and J. Hermon-Taylor. 1989. Sequence and characteristics of IS900, an insertion element identified in a human Crohn's disease isolate of Mycobacterium paratuberculosis. Nucleic Acids Res. 17:9063-9073.
3. Kunze, Z.M., S. Wall, R. Appelberg, M.T. Silva, F. Portaels and J.J. McFadden. 1991. Biologically distinct subtypes of Mycobacterium avium differ in possession of insertion sequence IS901. J. Clin. Microbiol. 30:2366-2372.
4. Levy-Frebault, V.V., M.F. Thorel, A. Varnerot and B. Gicquel. 1989. DNA polymorphism in Mycobacterium paratuberculosis, "wood pigeon mycobacteria", and related mycobacteria analyzed by field inversion gel electrophoresis. J Clin. Microbiol. 27:2823-2826.
5. Park, J.H., J.-C. Song, M.H. Kim, D.S. Lee, and C.-H. Kim. 1994. Determination of genome size and a preliminary physical map of an extreme alkaliphile, Micrococcus sp. Y-1, by pulsed-field gel electrophoresis. Microbiology 140:2247-2250
6. Schwartz, D.C., W. Saffran, J. Welsh, R. Haas, M. Goldenberg and C.R. Cantor. 1983. New techniques for purifying large DNA and studying their properties and packing. Cold Spring Harbor. Symp. Quant. Biol. 47:189-195.
7. Varnerot, A., F. Clement, M. Gheorghiu, and V. Vincent-Levy-Frebault. 1992. Pulsed-field gel electrophoresis of representatives of Mycobacterium tuberculosis and Mycobacterium bovis BCG strains. FEMS Microbiol. Lett. 98:155-160.
8. Zhang, Y., G. Mazurek, M.D. Cave, K.D. Eisenach, Y. Pang, D.T. Murphy and R.J. Wallace. 1992. DNA polymorphisms in strains of Mycobacterium tuberculosis analyzed by pulsed-field gel electrophoresis: a tool for epidemiology. J. Clin. Microbiol. 30:1551-1556.