Cloning of size-selected human immunoglobulin heavy-chain rearrangements from third complementarity-determining region fingerprint profiles

BioTechniques

Volumn 20, Issue 1, 1996, Pages 78-87

  Raaphorst, Frank M ^a   Tami, Joseph ^a   Sanz, Ignacio E ^a,b  

^a UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

^b Department of Medicine ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; IMMUNOGLOBULIN HEAVY CHAIN; T LYMPHOCYTE RECEPTOR;

ADULT; ARTICLE; B LYMPHOCYTE; DNA FINGERPRINTING; DNA SEQUENCE; FETUS; GENE AMPLIFICATION; GENE REARRANGEMENT; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOGLOBULIN GENE; MOLECULAR CLONING; MONONUCLEAR CELL; POLYMERASE CHAIN REACTION; SILVER STAINING;

EID: 13344260683     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96201st02     Document Type: Article

References (28)

1. H fusions. Proc. Natl. Acad. Sci USA 79:4118-4122.
2. Alzari, P.M., M.B. Lascombe and R.J. Poljak. 1988. Three-dimensional structure of antibodies. Annu Rev. Immunol. 6:555-580.
3. Braun, J., L. Berberian, L. King, I. Sanz and H.L. Govan III. 1992. Restricted use of fetal VH3 immunoglobulin genes by unselected B cells in the adult. Predominance of 56p1-like VH genes in common variable immunodeficiency. J. Clin. Invest. 89:1395-1402.
4. β-gene families, and phenotypically resemble memory T cells. Proc. Natl. Acad. Sci. USA 90:11787-11791.
5. Caignard, A., P.Y. Dietrich, V. Morand, A. Lim, C. Pannetier, A.M. Leridant, T. Hercend, J. Even, P. Kourilsky and F. Triebel. 1994. Evidence for T-cell clonal expansion in a patient with squamous cell carcinoma of the head and neck. Cancer Res. 54:1292-1297.
6. Coehet, M., C. Pannetier, A. Regnault, S. Darche, C. Leclerc and P. Kourilsky. 1992. Molecular detection and in vivo analysis of the specific T-cell response to a protein antigen. Eur. J. Immunol. 22.2639-2647.
7. Cottrez, F., C. Auriault, A. Capron and H. Groux. 1994. Analysis of the Vβ specificity of superantigen activation with a rapid and sensitive method using RT PCR and an automatic DNA analyser. J. Immunol Methods 172:85-94.
8. Deane, M. and J.D. Norton. 1991. Immunoglobulin gene 'fingerprinting': an approach to analysis of B lymphoid clonality in lymphoproliferative disorders. Br. J. Hematol. 77:274-281.
9. Desravines, S. and E. Hsu. 1994. Measuring CDR3 length variability in individuals during ontogeny. J Immunol Methods 168:219-225.
10. Dietrich, P.Y., A. Caignard, A. Lim, V. Chung, J.L. Pico, C. Pannetier, P. Kourilsky, T. Hercend, J. Even and F. Triebel. 1994 In vivo T-cell clonal amplification at time of acute graft-versus-host disease. Blood 84:2815-2820.
11. Gaudin, C., P.-Y. Dietrich, S. Robache, M. Guillard, B. Escudier, M.-J. Terrier Lacombe, A. Kumar, F. Triebel and A. Caignard. 1995. In vivo local expansion of clonal T cell subpopulations in renal cell carcinoma. Cancer Res 55:685-690
12. Gorski, J., M. Yassai, X. Zhu, B. Kissela, C. Keever and N. Flomenberg. 1994. Circulating T cell repertoire complexity in normal individuals and bone marrow recipients analyzed by CDR3 spectratyping Correlation with immune status. J. Immunol. 152:5109-5119.
13. Huang, C., A.K. Stewart, R.S. Schwartz and B.D. Stollar. 1992. Immunoglobulin heavy chain gene expression in peripheral blood B lymphocytes. J. Clin. Invest. 89:1331-1343.
14. Jäck, H.-M., J. Berg and M. Wabl. 1989. Translation affects immunoglobulin mRNA stability. Eur. J. Immunol. 19.843-847.
15. Kabat, E.A., T.T. Wu, H.M. Perry, K.S. Gottesman and C. Foeller. 1991. Sequences of proteins of immunological interest. 5th ed. (NIII Publication No. 91-2342). US Department of Health and Human Services, Public Health Service. National Institutes of Health, Bethesda, MD.
16. Kallenbach, S., N. Doyen, M. Fanton d'Andon and F. Rougeon. 1992. Three lymphoid-specific factors account for all junctional diversity characteristic of somatic assembly of T-cell receptor and immunoglobulin genes. Proc. Natl. Acad. Sci. USA 89:2799-2803
17. Lewis, S., A. Gifford and D. Baltimore. 1985. DNA elements are asymetrically joined during the site-specific recombination of kappa immunoglobulin genes. Science 228:677-685.
18. Padlan, E.A. 1994. Anatomy of the antibody molecule. Mol. Immunol. 37:169-217.
19. Pannetier, C., M. Cochet, S. Darche, A. Casrouge, M. Zöller and P. Kourilsky. 1993. The sizes of the CDR3 hypervariable regions of the murine T-cell receptor β chains vary as a function of the recombined germ-line segments. Proc Natl. Acad. Sci. USA 90:4319-4323.
20. Puisieux, I., J. Even, C. Pannetier, F. Jotereau, M. Favrot and P. Kourilsky. 1994. Oligoclonality of tumor-infiltrating lymphocytes from human melanomas. J. Immunol. 153:2807-2818.
21. Raaphorst, F.M., E. Timmers, M. Kenter, M.J.D. van Tol, J.M. Vossen and R.K.B. Schuurman. 1992. Restricted utilization of germline VH3 elements and short diverse third complementarity-determining regions (CDR3) in human fetal B lymphocyte immunoglobulin heavy chain rearrangements Eur J. Immunol. 22:247-251
22. Raaphorst, F.M., J. van Bergen, R. Langlois-van den Bergh, M. van der Keur, R. de Krijger, J. Bruining, M.J.D. van Tol, J.M. Vossen and P.J. van den Elsen. 1994, Usage of TCRAV and TCRBV gene families in human fetal and adult TCR rearrangements. Immunogenetics 39:343-350.
23. Raju, R., B.L. Hoppe, D. Navaneetham and B.M. Conti-Fine. 1995. Rapid method for the elution and analysis of PCR products separated on high resolution acrylamide gels. BioTechniques 18:32-36.
24. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
25. Sanz, I. 1991. Multiple mechanisms participate in the generation of diversity of human H chain CDR3 regions. J. Immunol. 147:1720-1729.
26. Schroeder, H.W., Jr. and J.Y. Wang. 1990. Preferential utilization of conserved immunoglobulin heavy chain variable gene segments dunng human fetal life. Proc. Natl. Acad. Sci. USA 57:6146-6150.
27. Tonegawa, S. 1983. Somatic generation of antibody diversity. Nature 302:575-581.
28. Uckun, F.M. 1990. Regulation of human B-cell ontogeny. Blood 76:1908-1923.