Soluble expression and affinity purification of functional domain of human acetylcholine receptor α-subunit by the modulation of maltose binding protein

Biotechnology Letters

Volumn 26, Issue 23, 2004, Pages 1765-1769

  Li, Zhuo Yu ^a   Li, Yan Jun ^a   Guo, Chen Yun ^a   Shi, Ye Wei ^a   Xu, Ming Qun ^b   Trommer, Wolfgang E ^c   Yuan, Jing Ming ^a  

^a SHANXI UNIVERSITY   (China)

^b NEW ENGLAND BIOLABS   (United States)

^c Rheinland Pfälzische Technische Universität Kaiserslautern Landau   (Germany)

Author keywords

Acetylcholine receptor; Affinity purification; Functional domain; Fusion expression; Immunore activities

Indexed keywords

CELLS; CHROMATOGRAPHY; IMMUNOLOGY; PROTEINS; PURIFICATION;

ACETYLCHOLINE RECEPTOR (ACHR0; AMYLOSE; BLOT ANALYSIS;

BIOTECHNOLOGY;

CANCER PROCOAGULANT; CARRIER PROTEIN; CYSTEINE PROTEINASE; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; MALTOSE-BINDING PROTEIN; NICOTINIC RECEPTOR; TUMOR PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING SITE; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN TERTIARY STRUCTURE; SOLUBILITY;

BINDING SITES; CARRIER PROTEINS; CHROMATOGRAPHY, AFFINITY; CYSTEINE ENDOPEPTIDASES; ESCHERICHIA COLI; HUMANS; NEOPLASM PROTEINS; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, NICOTINIC; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

ESCHERICHIA COLI; TORPEDO;

EID: 13244255678     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10529-004-4605-x     Document Type: Article

References (8)

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