Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs

Pesquisa Veterinaria Brasileira

Volumn 24, Issue 4, 2004, Pages 199-202

  Minho, Alessandro Pelegrine ^a   Freire, Roberta Lemos ^a   Vidotto, Odilon ^a   Gennari, Solange Maria ^a,b   Marana, Elizabete Marangoni ^a   Garcia, João Luis ^a,c   Navarro, Italmar Teodorico ^a  

^a STATE UNIVERSITY OF LONDRINA   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

^c UNIVERSIDADE ESTADUAL DO CENTRO OESTE   (Brazil)

Author keywords

Antibodies; Indirect fluorescent antibody test (IFAT); Modified agglutination test (MAT); Serology; Swine; Toxoplasma gondii

Indexed keywords

ACCURACY; AGGLUTINATION TEST; ANIMAL EXPERIMENT; ANTIBODY DETECTION; ARTICLE; CONFIDENCE INTERVAL; EXPERIMENTAL ANIMAL; FLUORESCENT ANTIBODY TECHNIQUE; MALE; METHODOLOGY; NONHUMAN; PROTOZOAL INFECTION; SENSITIVITY ANALYSIS; SERUM; SWINE; SWINE DISEASE;

ANIMALIA; SUS SCROFA; TOXOPLASMA GONDII;

EID: 13244253790     PISSN: 0100736X     EISSN: None     Source Type: Journal    
DOI: 10.1590/S0100-736X2004000400005     Document Type: Article

References (30)

1. Aranda F.S., Galisteo Jr A.J., Hiramoto R.M., Cardoso R.P., Meireles L.R., Miguel O. & Andrade Jr H.F. 2000. The prevalence and avidity of Toxoplasma gondii IgG antibodies in pigs from Brazil and Peru. Vet. Parasitol. 91:23-32.
2. Camargo M.E. 1973. Introdução às técnicas de imunofluorescência. Revta Bras. Patol. Clinica 10:143-171.
3. Camargo M.E. 1964. Improved technique of indirect immunofluorescence for serological diagnosis of toxoplasmosis. Revta Inst. Med. Trop., São Paulo, 6:117-118.
4. Dean A.G., Dean I.A. & Burton A.H. 1992. EPI INFO version 6.04: a world processing, data base and statistic program for epidemiology on microcomputers. Center for Disease Control, Atlanta, Georgia, USA. 302p. Disponível em http//:www.cdc.gov
5. Desmonts G. & Remington J.S. 1980. Direct agglutination test for diagnosis of Toxoplasma infection: method for increasing sensitivity and specificity. J. Clin. Microbiol. 11:562-568.
6. Dubey J. P. 1986. Toxoplasmosis. J. Am. Vet. Assoc. 189:166-170.
7. Dubey J.P. & Desmonts G. 1987. Serological responses of equids fed with Toxoplasma gondii oocysts. Equine Vet. J. 48:1239-1243.
8. Dubey J.P., Thulliez P., Weigel R.M., Andrews C.D., Lind P. & Powell E.C. 1995. Sensitivity and specificity of various serologic tests for detection of Toxoplasma gondii infection in naturally infected sows. Am. J. Vet Res. 56:1030-1036.
9. Dubey J.P., Lindsay D.S., Adams D.S., Gay J.M., Baszler T.V., Blagburn B.L. & Thulliez P. 1996. Serologic responses of cattle and others animals infected with Neospora caninum. Am. J. Vet. Res. 57:329-336.
10. Dubey J.P., Andrews C.D., Thulliez P., Lind P. & Kwok O.C.H. 1997. Long-term humoral antibody responses by various serologic tests in pigs orally inoculated with oocysts of four strains of Toxoplasma gondii. Vet. Parasitol. 68:41-50.
11. Dubey J.P. 1997. Validation of the specificity of the modified agglutination test for toxoplasmosis in pigs. Vet. Parasitol. 71:307-310.
12. Freire R.L., Navarro I.T, Bracarense A.P.F.R.L. & Gennari, S.M. 2003. Vaccination of pigs with Toxoplasma gondii antigens incorporated in immunostimulating complexes (iscoms). Arq. Bras. Med. Vet. Zoot. 55: 388-396.
13. Gamble H.R., Brady R.C. & Dubey J.P. 1999. Prevalence of Toxoplasma gondii infection in domestic pigs in the New England states. Vet. Parasitol. 82:129-136.
14. Garcia J.L., Navarro I.T., Ogawa L. & Oliveira R.C. 1999. Soroprevalência do T. gondii, em suínos, bovinos, ovinos e eqüinos e sua correlação com humanos, felinos e caninos, oriundos de propriedades rurais do norte do Paraná-Brasil. Ciência Rural, Santa Maria, 29:91-97.
15. Garcia J.L. 2003. Avaliação da Resposta Imune em suínos, com vacina de fração antigênica (roptrias e grânulos densos) de Toxoplasma gondii incorporados a complexos imunoestimulantes (iscoms). Tese (Doutorado), VPS, FMVZ-USP, São Paulo.
16. Gart J.J.& Buck A.A. 1966. Comparison of a screening test and a reference test in epidemiologic studies. Am. J. Epidemiol. 83:593-602.
17. Gomez-Marin J.E., Montoya-De-Londono M.T., Castano-Osorio J.C., Heine F.A, Duque A.M., Chemla C., Aubert D., Bonhomme A. & Pinon J.M. 2000. Frequency of specific anti-Toxoplasma gondii IgM, IgA and IgE in Colombian patients with acute and chronic ocular Toxoplasmosis. Mem. Inst. Oswaldo Cruz 95:89-94.
18. Hirvelã-Koski V. 1990. Evaluation of ELISA for the detection of Toxoplasma antibodies in swine sera. Acta Vet. Scand. 31:413-422.
19. Kreier J. P. 1993. Parasitic Protozoa. 2nd Edition. Academic Press, San Diego, p.5-57.
20. Landis J.R. & Kock G.G. 1977. The measurement of observer agreement for categorical data. Biometrics 33:159-174.
21. Navarro I.T., Vidotto O., Giraldi N. & Freire, R. L. 1992. Toxoplasma gondii. Isolamento a partir de carne e cérebro de suínos comercializados na região de Londrina, PR. Semina 13:15-18.
22. Nicolle C. & Manceaux L. 1909. Sur un protozoaire nouveau du gondii. Compte Rendu H. Acad. Sci. 148:369-372.
23. Oksanen A., Tryland M., Johnsen K. & Dubey J.P. 1998. Serosurvey of Toxoplasma gondii in North atlantic marine mammals by the use of agglutination test employing whole taquizoites and dithiothreitol. Comp.Immunol. Microbiol.& Infect. Dis. 21:107-114.
24. Roberts F. & Mcleod R. 1999. Pathogenesis of toxoplasmic retinochoroiditis. Parasitol. Today 15:51-57.
25. Souza W.J.S. 1995. Epidemiologia da toxoplasmose. Avaliação sorológica em suínos e trabalhadores de abatedouros da mesma região do Grande Rio de Janeiro. Tese (Doutorado), UFRRJ, Rio de Janeiro.102 p.
26. Waltman W.D., Dreesen D.W., Prickett M.D., Blue J.L. & Oliver D.G. 1984. Enzime-linked immunossorbent assay for the detection of toxoplasmosis in swine: Interpreting assay results and comparing with other serologic tests. Am. J. Vet. Res. 45:1719-1725.
27. Work K., Eriksen L., Fennestad K.L., Moller T. & Siim J.C. 1970. Experimental toxoplasmosis in pregnant sows. Acta. Path. Microbiol. Scand. 78:129-139.
28. Vidotto O., Costa A.J. & Balarin M.R.S. 1987. Toxoplasmose experimental em porcas gestantes. I. Observações clínicas e hematológicas. Arq. Bras. Med. Vet. Zoot. 39:623-639.
29. Vidotto O., Navarro I.T., Giraldi N., Freire R.L. & Mitsuka R. 1990. Estudos epidemiológicos da toxoplasmose em suínos da região de Londrina, PR. Semina 11:53-59.
30. Vidotto O. 1992. Toxoplasmose: epidemiologia e importância da doença na sau̧de animal. Semina 13:69-75.