A faster way to make GFP-based biosensors: Two new transposons for creating multicolored libraries of fluorescent fusion proteins

BMC Biotechnology

Volumn 4, Issue , 2004, Pages

  Sheridan, Douglas L ^a   Hughes, Thomas E ^b  

^a YALE UNIVERSITY   (United States)

^b MONTANA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSENSORS; CALCIUM; DNA; FLUORESCENCE; LIVING SYSTEMS STUDIES; POSITIVE IONS;

FUSION PROTEINS; GREEN FLUORESCENT PROTEIN (GFP); INTEGRAL MEMBRANE PROTEINS; PROTEIN CODING SEQUENCES;

PROTEINS;

CALCIUM CHANNEL; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MEMBRANE PROTEIN; PHOTOPROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BIOSENSOR; CONTROLLED STUDY; EMBRYO; GENE INSERTION; GENE MAPPING; GENETIC CODE; HUMAN; HUMAN CELL; MOLECULAR CLONING; PROTEIN TARGETING; SEQUENCE ANALYSIS; TRANSPOSON; CELL LINE; CHEMISTRY; COLOR; GENETIC PROCEDURES; GENETICS; KIDNEY; METABOLISM; METHODOLOGY; PRENATAL DEVELOPMENT;

INSERTION SEQUENCES; MAMMALIA; TRANSPOSONS;

BIOSENSING TECHNIQUES; CELL LINE; CLONING, MOLECULAR; COLOR; DNA TRANSPOSABLE ELEMENTS; GREEN FLUORESCENT PROTEINS; HUMANS; KIDNEY; LUMINESCENT PROTEINS; RECOMBINANT FUSION PROTEINS;

EID: 13244250999     PISSN: 14726750     EISSN: None     Source Type: Journal    
DOI: 10.1186/1472-6750-4-17     Document Type: Article

References (17)

1. Siegel MS, Isacoff EY: A genetically encoded optical probe of membrane voltage. Neuron 1997, 19:735-741.
2. Ataka K, Pieribone VA: A genetically targetable fluorescent probe of channel gating with rapid kinetics. Biophys J 2002, 82:509-516.
3. Sakai R, Repunte-Canonigo V, Raj CD, Knopfel T: Design and characterization of a DNA-encoded, voltage-sensitive fluorescent protein. Eur J Neurosci 2001, 13:2314-2318.
4. Miyawaki A, Griesbeck O, Heim R, Tsien RY: Dynamic and quantitative Ca2+ measurements using improved cameleons. Proc Natl Acad Sci U S A 1999, 96:2135-2140.
5. Barak LS, Ferguson SS, Zhang J, Caron MG: A beta-arrestin/green fluorescent protein biosensor for detecting G protein-coupled receptor activation. J Biol Chem 1997, 272: 7497-27500.
6. Merkulov GV, Boeke JD: Libraries of green fluorescent protein fusions generated by transposition in vitro. Gene 1998, 222:213-222.
7. Biondi RM, Baehler PJ, Reymond CD, Veron M: Random insertion of GFP into the cAMP-dependent protein kinase regulatory subunit from Dictyostelium discoideum. Nucleic Acids Res 1998, 26:4946-4952.
8. Sheridan DL, Berlot CH, Robert A, Inglis FM, Jakobsdottir KB, Howe JR, Hughes TE: A new way to rapidly create functional, fluorescent fusion proteins: random insertion of GFP with an in vitro transposition reaction. BMC Neurosci 2002, 3:7.
9. Zhou M, Bhasin A, Reznikoff WS: Molecular genetic analysis of transposase-end DNA sequence recognition: cooperativity of three adjacent base-pairs in specific interaction with a mutant Tn5 transposase. J Mol Biol 1998, 276:913-925.
10. Goryshin IY, Reznikoff WS: Tn5 in vitro transposition. J Biol Chem 1998, 273:7367-7374.
11. Zheng J, Shen W, He DZ, Long KB, Madison LD, Dallos P: Prestin is the motor protein of cochlear outer hair cells. Nature 2000, 405:149-155.
12. Mehdi H, Ono E, Gupta KC: Initiation of translation at CUG, GUG, and ACG codons in mammalian cells. Gene 1990, 91: 73-178.
13. Manoil C, Traxler B: Insertion of in-frame sequence tags into proteins using transposons. Methods 2000, 20:55-61.
14. Mignery GA, Newton CL, Archer B. T., 3rd, Sudhof TC: Structure and expression of the rat inositol 1,4,5-trisphosphate receptor. J Biol Chem 1990, 265:12679-12685.
15. Mizuno H, Sawano A, Eli P, Hama H, Miyawaki A: Red fluorescent protein from Discosoma as a fusion tag and a partner for fluorescence resonance energy transfer. Biochemistry 2001, 40:2502-2510.
16. Sacchetti A, Subramaniam V, Jovin TM, Alberti S: Oligomerization of DsRed is required for the generation of a functional red fluorescent chromophore. FEBS Lett 2002, 525:13-19.
17. Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A: A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 2002, 20:87-90.