Dedifferentiation of conditionally immortalized hepatocytes with long-term in vitro passage

Experimental and Molecular Medicine

Volumn 32, Issue 1, 2000, Pages 29-37

  Kim, Byung Ho ^a   Sung, Se Ra ^a   Choi, Eun Hee ^a   Kim, Young Il ^a   Kim, Kyeong Jin ^a   Dong, Seok Ho ^a   Kim, Hyo Jong ^a   Chang, Young Woon ^a   Lee, Joung Il ^a   Chang, Rin ^a  

^a Kyung Hee University Hospital   (South Korea)

Author keywords

Chromosome; Conditional immortalization; Liver specific differentiation; Rat hepatocytes; Telomerase; Temperature sensitive SV40 large T antigen

Indexed keywords

ALBUMIN; PROTEIN P53; UREA; VIRUS LARGE T ANTIGEN;

ANCHORAGE INDEPENDENT GROWTH; ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL IMMORTALIZATION; CELL STRUCTURE; CHROMOSOME ANALYSIS; CHROMOSOME NUMBER; ENZYME ACTIVITY; LIVER CELL CULTURE; NONHUMAN; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; RAT; SIMIAN VIRUS 40; TEMPERATURE SENSITIVE MUTANT;

ANIMALIA; SIMIAE; SIMIAN VIRUS; SIMIAN VIRUS 40;

EID: 12944254593     PISSN: 12263613     EISSN: 20926413     Source Type: Journal    
DOI: 10.1038/emm.2000.6     Document Type: Article

References (26)

1. Brown, P. C. and Papaconstantinou, J. (1979) Coordinated modulation of albumin synthesis and mRNA levels in cultured hepatoma cells by hydrocortisone and cyclic AMP analogs. J. Biol. Chem. 254: 9379-84
2. Chou, J. Y. (1983) Temperature-sensitive adult liver cell line dependent on glucocorticoid for differentiation. Mol. Cell. Biol. 3: 1013-20
3. Clayton, D. F. and Darnell, J. E. Jr. (1983) Changes in liver-specific compared to common gene transcription during primary culture of mouse hepatocytes. Mol. Cell. Biol. 3:1552-61
4. Clayton, D. F., Weiss, M. and Darnell, J. E. Jr. (1985) Liver-specific RNA metabolism in hepatoma cells: variations in transcription rates and mRNA levels. Cell Biol. 5: 2633-41
5. Dich, J., Vind, C. and Grunnet, N. (1988) Long-term culture of hepatocytes: effect of hormones on enzyme activities and metabolic capacity. Hepatology 8: 39-45
6. Fox, I. J., Roy Chowdhury, N., Gupta, S., Kondapalli, R., Schilsky, M., Stockert, R. J. and Roy Chowdhury, J. (1995) Conditional Immortalization of Gunn rat hepatocytes: an ex vivo model for evaluating methods for bilirubin-UDP-glucuronosyltransferase gene transfer. Hepatology 21: 837-46
7. Greider, C. W. (1991) Chromosome first aid. Cell 67: 645-7
8. Guguen-Guillouzo, C. and Guillouzo, A. (1983a) Modulation of functional activities in cultured rat hepatocytes. Mol. Cell. Biol. 53/54: 35-56
9. Guguen-Guillouzo, C., Clement, B., Baffet, G., Beaumont, C., Morel-Chany, E., Glaise, D. and Guillouzo, A. (1983b) Maintenance and reversibility of active albumin secretion by adult rat hepatocytes co-cultured with another liver epithelial cell type. Exp. Cell Res. 143: 47-54
10. Harley, C. B., Kim, N. W., Prowse, K. R., Weinrich, S. L., Hirsch, K. S., West, M. D., et al. (1994) Telomerase, cell immortality and cancer. Cold Spring Harb. Symp. Quant. Biol. 59: 307-15
11. Jat, P. S. and Sharp, P. A. (1989) Cell lines established by a temperature-sensitive simian virus 40 large T-antigen gene are growth restricted at the nonpermissive temperature. Mol. Cell. Biol. 9: 1672-81
12. Kim, N. W., Piatyszek, M. A., Prowse, K. R., Harley, C. B., West, M. D., Ho, P. L. C., Coviello, G. M., Wright, W. E., Weinrich, S. L. and Shay, J. W. (1994) Specific association of human telomerase activity with immortal cells and cancer. Science 266: 2011-15
13. Kipling, D. and Cooke, H. J. (1990) Hypervariable ultra-long telomeres in mice. Nature 347: 400-2
14. Kowalski-Saunders, P. W. J., Winwood, P. J., Arthur, M. J. P. and Wright, R. (1992) Reversible inhibition of albumin production by rat hepatocyte maintained on a laminin- rich gel in response to secretory products of Kupffer cells and cytokines. Hepatology 16: 733-41
15. Lane, D. P. (1994) The regulation of p53 function: Steiner Award Lecture. Int. J. Cancer 57: 623-7
16. Levine, A. J. (1990) Tumor suppressor genes. BioEssays 12: 60-6
17. Macpherson, I. and Montagnier, L. (1964) Agar suspension culture for the selective assay of cells transformed by polyomavirus. Virology 23: 291-4
18. Miura, M., Karasaki, Y., Tetsuya, A., Higashi, K., Ikemura, K. and Gotoh, S. (1998) Prompt activation of telomerase by chemical carcinogens in rats detected with a modified TRAP assay. Biochem. Biophys. Res. Commun. 246: 13-9
19. Morin, O. and Normand, C. (1986) Long-term maintenance of hepatocyte functional activity in co-culture: requirements for sinusoidal endothelial cells and dexamethasone. J. Cell Physiol. 129: 103-10
20. Nakamura, J., Okamoto, T., Schumacher, I. K., Tabei, I., Roy Chowdhury, N., Roy Chowdhury, J. and Fox, I. J. (1997) Treatment of surgically induced acute liver failure by transplantation of conditionally immortalized hepatocytes. Transplantation 15: 1541-7
21. Rojkind, M., Gatmatian, Z., Mackensen, S., Giambrone, M. A., Ponce, P. and Reid, L. M. (1980) Connective tissue biomatrix: its isolation and utilization for long-term cultures of normal rat hepatocytes. J. Cell Biol. 87: 255-63
22. Schumacher, I. K., Okamoto, T., Kim, B-H., Roy Chowdhury, N., Roy Chowdhury, J. and Fox, I. J. (1996) Transplantation of conditionally immortalized hepatocytes to treat hepatic encephalopathy. Hepatology 24: 337-43
23. Seglen, P. O. (1973) Preparation of rat liver cells. III. Enzymatic requirements for tissue dispersion. Exp. Cell Res. 82: 391-398
24. Waxman, D. J., Morrissey, J. J., Naik, S. and Jauregui, H. O. (1990) Phenobarbital induction of cytochromes P-450. Biochem. J. 271: 113-9
25. Woodworth, C. D., Kreider, J. W., Mengel, L., Miller, T., Meng, Y. and Isom, H. C. (1988) Tumorigenicity of simian virus 40-hepatocyte cell lines: effect of in vitro and in vivo passage on expression of liver-specific genes and oncogenes. Mol. Cell. Biol. 8: 4492-501
26. Wright, W., Pereira-Smith, O. and Shay, J. (1989) Reversible cellular senescence: implications for immortalization of normal human diploid fibroblasts. Mol. Cell. Biol. 9: 3088-92