Expression screen by enzyme-linked immunofiltration assay designed for high-throughput purification of affinity-tagged proteins

Analytical Biochemistry

Volumn 317, Issue 2, 2003, Pages 255-258

  Kery, Vladimir ^a   Savage, Justin R ^a   Widjaja, Kartika ^a   Blake, B Kelly ^a   Conklin, David R ^a   Ho, Yew Seng J ^a   Long, Xinghua ^a   Von Rechenberg, Moritz ^a   Zarembinski, Thomas I ^a   Boniface, J Jay ^a  

^a Prolexys Pharmaceuticals Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; ENZYMES; ESCHERICHIA COLI; PROTEOMICS;

GLUTATHIONE S-TRANSFERASES; HIGH THROUGHPUT PURIFICATION; HIGH-THROUGHPUT ANALYSIS; HORSERADISH PEROXIDASE; PROTEIN PURIFICATION; QUANTITATIVE EVALUATION; SECONDARY ANTIBODIES; TETRAMETHYLBENZIDINE;

PURIFICATION;

ANALYTE; ANTIBODY; GLUTATHIONE TRANSFERASE; HORSERADISH PEROXIDASE; HYBRID PROTEIN; TETRAMETHYLBENZIDINE; UNCLASSIFIED DRUG;

ABSORPTION; ACCURACY; ANTIGEN BINDING; ARTICLE; BACTERIAL MEMBRANE; BACTERIUM; BIOLOGICAL MONITORING; CELL LYSATE; CONTROLLED STUDY; ENZYME ASSAY; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOFILTRATION ASSAY; ESCHERICHIA COLI; EVALUATION; EXTRACT; INTERMETHOD COMPARISON; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; QUANTITATIVE ANALYSIS; SCREENING TEST; WESTERN BLOTTING;

ARMORACIA RUSTICANA; ESCHERICHIA COLI;

EID: 12444346851     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(03)00116-7     Document Type: Article

References (3)

1. Braun P., Hu Y., Shen B., Halleck A., Koundinya M., Harlow E., LaBaer J. Proteome-scale purification of human proteins from bacteria. Proc. Natl. Acad. Sci. USA. 99:2002;2654-2659.
2. Draveling C., Ren L., Haney P., Zeisse D., Qoronfleh M.W. SwellGel: an affinity chromatography technology for high-capacity and high-throughput purification of recombinant-tagged proteins. Prot. Expr. Purif. 22:2001;359-366.
3. Habig W.H., Pabst M.J., Jakoby W.B. Glutathione S-transferases: the first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249:1974;7130-7139.