Rapid monitoring of microbial contamination on herbal medicines by fluorescent staining method

Letters in Applied Microbiology

Volumn 40, Issue 2, 2005, Pages 128-132

  Nakajima, K ^a   Nonaka, K ^a   Yamamoto, K ^b   Yamaguchi, N ^b   Tani, K ^b   Nasu, Masao ^b  

^a Hiya Pharmaceutical Co Ltd   (Japan)

^b OSAKA UNIVERSITY   (Japan)

Author keywords

6 carboxyfluorescein diacetate; Crude drugs; Direct counting; Fluorescent vital staining; Microbial monitoring

Indexed keywords

ENZYME ACTIVITY; ESTERS; FLUORESCENCE; MEDICINE; PLANT EXTRACTS;

6-CARBOXYFLUORESCEIN DIACETATE; CARBOXYFLUORESCEIN DIACETATE; CRUDE DRUGS; DIRECT COUNTING; FLUORESCENT STAINING; FLUORESCENT VITAL STAINING; HERBAL MEDICINES; MICROBIAL MONITORING; STAINING METHOD; VITAL STAINING;

BACTERIA;

4',6 DIAMIDINO 2 PHENYLINDOLE; 6 CARBOXYFLUORESCEIN; AQUILARIAE LIGNUM; BEZOAR BOVIS; CHINESE DRUG; FEL URSI; GINSENG EXTRACT; HERBACEOUS AGENT; MUSK; UNCLASSIFIED DRUG;

DETECTION METHOD;

ARTICLE; BACTERIAL CELL; BACTERIAL COLONIZATION; BACTERIUM CONTAMINATION; CHINESE MEDICINE; COLONY FORMING UNIT; DRUG CONTAMINATION; ENZYME ACTIVITY; FLUORESCENCE; MICROBIAL CONTAMINATION; NONHUMAN; PLATE COUNT; STAINING; TRADITIONAL MEDICINE;

BACTERIA (MICROORGANISMS); PANAX GINSENG;

EID: 12444341808     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2004.01643.x     Document Type: Article

References (22)

1. Amann, R.I., Ludwig, W. and Schleifer, K.-H. (1995) Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiological Reviews 59, 143-169.
2. Bloem, J., Veninga, M. and Shepherd, J. (1995) Fully automatic determination of soil bacterium numbers, cell volumes, and frequencies of dividing cells by confocal laser scanning microscopy and image analysis. Applied and Environmental Microbiology 61, 926-936.
3. Bunthof, C.J. and Abee, T. (2002) Development of a flow cytometric method to analyze subpopulation of bacteria in probiotic products and dairy starters. Applied and Environmental Microbiology 68, 2934-2942.
4. Davey, H.M. and Kell, D.B. (1996) Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analysis. Microbiological Reviews 60, 641-696.
5. Dive, C., Cox, H., Watson, J.V. and Workman, P. (1988) Polar fluorescein derivatives as improved substrate probes for flow cytoenzymological assay of cellular esterases. Molecular and Cellular Probes 2, 131-145.
6. Hoefel, D., Grooby, W.L., Moris, P.T., Andrews, S. and Saint, C.P. (2003) Enumeration of water-borne bacteria using viability assays and flow cytometry: a comparison to culture-based techniques. Journal of Microbiological Methods 55, 585-597.
7. Kawai, M., Yamaguchi, N. and Nasu, M. (1999) Rapid enumeration of physiologically active bacteria in purified water used in the pharmaceutical manufacturing process. Journal of Applied Microbiology 86, 496-504.
8. Kimura, S. (1997) Use of the radiation method for sterilization of herbal medicines. Journal of Antibacterial and Antifungal Agents Japan 25, 475-479.
9. Mignon-Godefroy, K., Guillet, J.-G. and Butor, C. (1997) Solid phase cytometry for detection of rare events. Cytometry 27, 336-344.
10. Muyzer, G., de Wall, E.C. and Uitterlinden, A.G. (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Applied and Environmental Microbiology 59, 695-700.
11. Ogawa, M., Tani, K., Yamaguchi, N. and Nasu, M. (2003) Development of multicolour digital image analysis system to enumerate actively respiring bacteria in natural river water. Journal of Applied Microbiology 95, 120-128.
12. Porter, K.G. and Feig, Y.S. (1980) The use of DAPI for identifying and counting aquatic microflora. Limnology and Oceanography 25, 943-948.
13. Porter, J., Edwards, C. and Pickup, R. (1995) Direct measurements of natural planktonic bacterial community viability by flow cytometry. Applied and Environmental Microbiology 61, 2783-2786.
14. Posch, T., Pernthaler, J., Alfreider, A. and Psenner, R. (1997) Cell-specific respiratory activity of aquatic bacteria studied with the tetrazolium reduction method, Cyto-Clear slides, and image analysis. Applied and Environmental Microbiology 63, 867-873.
15. Pyle, B.H., Broadaway, S.C. and McFeters, G.A. (1999) Sensitive detection of Escherichia coli O157:H7 in food and water by immunomagnetic separation and solid-phase laser cytometry. Applied and Environmental Microbiology 65, 1966-1972.
16. Reynolds, D.T. and Fricker, C.R. (1999) Application of laser scanning for the rapid and automated detection of bacteria in water samples. Journal of Applied Microbiology 86, 785-795.
17. Rodriguez, G.G., Phipps, D., Ishiguro, K. and Ridgway, H.F. (1992) Use of fluorescent redox probe for direct visualization of actively respiring bacteria. Applied and Environmental Microbiology 58, 1801-1808.
18. Tani, K., Yamamoto, K., Yamaguchi, N. and Nasu, M. (1998) Rapid detection of bacteria on peppercorns by the fluorescent staining method. Journal of Antibacterial and Antifungal Agents Japan 26, 415-421.
19. Vives-Rego, J., Lebaron, P. and von Caron, G.N. (2000) Current and future applications of flow cytometry in aquatic microbiology. FEMS Microbiology Reviews 24, 429-448.
20. Yamaguchi, N. and Nasu, M. (1997) Flow cytometric analysis of bacterial respiratory and enzymatic activity in the natural aquatic environment. Journal of Applied Microbiology 83, 43-52.
21. Yamaguchi, N., Sasada, M., Yamanaka, M. and Nasu, M. (2003) Rapid detection of respiring Escherichia coli O157:H7 in apple juice, milk, and ground beef by flow cytometry. Cytometry 54A, 27-35.
22. Yokomaku, D., Yamaguchi, N. and Nasu, M. (2000) Improved direct viable count procedure for quantitative estimation of bacterial viability in freshwater environments. Applied and Environmental Microbiology 66, 5544-5548.