Selective inhibition of inducible cyclo-oxygenase-2 expression by antisense peptide nucleic acids in intact murine macrophages

Biotechnology and Applied Biochemistry

Volumn 38, Issue 1, 2003, Pages 61-69

  Scarfi, Sonia ^a   Giovine, Marco ^b   Pintus, Raffaela ^a   Millo, Enrico ^a   Clavarino, Emanuela ^a   Pozzolini, Marina ^a   Sturla, Laura ^c   Stock, Roberto Pablo ^d   Benatti, Umberto ^a   Damonte, Gianluca ^a  

^a UNIVERSITY OF GENOA   (Italy)

^b CNR   (Italy)

^c G GASLINI INSTITUTE   (Italy)

^d INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

Author keywords

Cyclo oxygenase 2 (COX 2); Murine macrophage; Peptide nucleic acid (PNA)

Indexed keywords

ENZYMES; HYDROPHOBICITY; NUCLEIC ACIDS; RNA;

MURINE MACROPHAGES;

BIOCHEMISTRY;

ADENINE; ANTISENSE OLIGONUCLEOTIDE; CYCLOOXYGENASE 2; GUANINE; LIPID; MESSENGER RNA; PEPTIDE NUCLEIC ACID; URACIL;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELL LYSATE; CELL STIMULATION; CELL TRANSPORT; CODON; DIFFUSION; ENZYME INHIBITION; ENZYME SYNTHESIS; HYDROPHOBICITY; MACROPHAGE; MEMBRANE PERMEABILITY; MICROSOME; MOLECULAR DYNAMICS; MONITORING; MOUSE; NONHUMAN; OPEN READING FRAME; PROTEIN EXPRESSION; PROTEIN FUNCTION; RABBIT; RADIOACTIVITY; RETICULOCYTE; STEREOCHEMISTRY; TRANSLATION INITIATION; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CELL LINE; CELL-FREE SYSTEM; CYCLOOXYGENASE 2; ENZYME INDUCTION; GENE EXPRESSION; ISOENZYMES; MACROPHAGES; MICE; MICROSCOPY, CONFOCAL; OLIGONUCLEOTIDES, ANTISENSE; PEPTIDE NUCLEIC ACIDS; PROSTAGLANDIN-ENDOPEROXIDE SYNTHASES; PROTEIN BIOSYNTHESIS; RABBITS; RETICULOCYTES; RNA, MESSENGER;

ANIMALIA; MURINAE; ORYCTOLAGUS CUNICULUS;

EID: 12444339903     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20030006     Document Type: Article

References (35)

1. Smith, W. L. and Marnett, L. J. (1991) Biochim. Biophys. Acta 1083, 1-17
2. O'Banion, K. M., Winn, V. D. and Young, D. A. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 4888-4892
3. Ristimaki, A., Garfinkel, S., Wessendorf, J., Maciag, T. and Hla, T. (1994) J. Biol. Chem. 269, 11769-11775
4. Hla, T. and Neilson, K. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 7384-7388
5. Xie, W. L., Chipman, J. G., Robertson, D. L., Erikson, R. L. and Simmons, D. L. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 2692-2696
6. Lee, S. H., Soyoola, E., Chanmugam, P., Hart, S., Sun, W., Zhong, H., Liou, S., Simmons, D. and Hwang, D. (1992) J. Biol. Chem. 267, 25934-25938
7. Goppelt-Struebe, M. (1995) Prostaglandins Leukot. Essent. Fatty Acids 52, 213-222
8. Habib, A., Creminon, C., Frobert, Y., Grassi, J., Pradelles, P. and Maclouf, J. (1993) J. Biol. Chem. 268, 23448-23454
9. Roy, R., Polgar, P., Wang, Y., Goldstein, R. H., Taylor, L. and Kagan, H. M. (1996) J. Cell. Biochem. 62, 411-417
10. DeWitt, D. L. (1991) Biochim. Biophys. Acta 1083, 121-128
11. Vane, J. R., Mitchell, J. A., Appleton, I., Tomlinson, A., Bishop-Bailey, D., Croxtal, J. and Willoughby, D. A. (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 2046-2050
12. Vane, J. R. (1971) Nat. New Biol. 231, 232-235
13. Khan, I., Al-Awadi, F. M. and Thomas, N. (2001) Antisense Nucl. Acid Drug Dev. 11, 199-207
14. Yamada, R., Sano, H., Hla, T., Hashiramoto, A., Kawahito, Y., Mukai, S., Khono, M., Tsubouchi, Y., Inoue, M., Komatsu, A. et al. (2000) Biochem. Biophys. Res. Commun. 269, 415-421
15. Nielsen, P. E., Egholm, M., Berg, R. H. and Buchardt, O. (1991) Science 254, 1497-1500
16. Cherny, D. Y., Belotserkovskii, B. P., Frank-Kamenetskii, M. D., Egholm, M., Buchardt, O., Berg, R. H. and Nielsen, P. E. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 1667-1670
17. Egholm, M., Buchardt, O., Christensen, L., Behrens, C., Freier, S. M., Driver, D. A., Berg, R., Kim, S. H., Norden, B. and Nielsen, P. E. (1993) Nature (London) 365, 566-568
18. Demidov, V. V., Yavnilovich, M. V., Belotserkovskii, B. P., Frank-Kamenetskii, M. D. and Nielsen, P. E. (1995) Proc. Natl. Acad. Sci. U.S.A. 92, 2637-2641
19. Soomets, U., Hallbrink, M. and Langel, U. (1999) Frontiers Biosci. 4, 782-786
20. Doyle, D. F., Braasch, D. A., Simmons, C. G., Janowski, B. A. and Corey D. R. (2001) Biochemistry 40, 53-64
21. Egholm, M., Buchardt, O., Nielsen, P. E. and Berg, R. H. (1992) J. Am. Chem. Soc. 114, 1895-1897
22. Demidov, V. V., Potaman, V. N., Frank-Kamenetskii, M. D., Egholm, M., Buchardt, O., Sonnichsen, S. H. and Nieslen, P. E. (1994) Biochem. Pharmacol. 48, 1310-1313
23. Scarfi, S., Gasparini, A., Damonte, G. and Benatti, U. (1997) Biochem. Biophys. Res. Commun. 236, 323-326
24. Scarfi, S., Giovine, M., Gasparini, A., Damonte, G., Millo, E., Pozzolini, M. and Benatti, U. (1999) FEBS Lett. 451, 264-268
25. Matsueda, J. R. and Stewart, J. M. (1981) Peptides 2, 45-50
26. Christensen, L., Fitzpatrick, R., Gildea, B., Petersen, K. H., Hansen, H. F., Koch, T., Egholm, M., Buchardt, O., Nielsen, P. E., Coull, J. and Berg, R. H. (1995) J. Pept. Sci. 1, 175-183
27. Giovine, M., Gasparini, A., Scarfi, S., Damonte, G., Sturla, L., Millo, E., Tonetti, M. and Benatti, U. (1998) FEBS Lett. 426, 33-36
28. Stock, R. P., Olvera, A., Sanchez, R., Saralegui, A., Scarfi, S., Sanchez-Lopez, R., Ramos, M. A., Boffa, L. C., Benatti, U. and Alagon, A. (2001) Nat. Biotechnol. 19, 231-234
29. Bradford, M. M. (1976) Anal. Biochem. 72, 248-254
30. Rao, C. V., Indranie, C., Simi, B., Manning, P. T., Connor, J. R. and Reddy, B. S. (2002) Cancer Res. 62, 165-170
31. Miysaka, N. and Hirata, Y. (1997) Life Sci. 61, 2073-2081
32. Wilson, K. T., Ramanujam, K. S., Mobley, H. L., Musselman, R. F., James, S. P. and Meltzer, S. J. (1996) Gastroenterology 111, 1524-1533
33. Dannhardt, G. and Kiefer, W. (2001) Eur. J. Med. Chem. 36, 109-126
34. Penco, S., Scarfi, S., Giovine, M., Damonte, G., Millo, E., Villaggio, B., Passalacqua, M., Pozzolini, M., Garrr̀, C. and Benatti, U. (2001) Biotechnol. Appl. Biochem. 34, 151-159
35. Hinz, B., Brune, K. and Pahl, A. (2000) Biochem. Biophys. Res. Commun. 272, 744-748