Detection of tumorigenic Agrobacterium strains from infected apple saplings by colony PCR with improved PCR primers

Journal of General Plant Pathology

Volumn 70, Issue 6, 2004, Pages 342-347

  Suzaki, Kouichi ^a   Yoshida, Kouji ^a   Sawada, Hiroyuki ^b  

^a NATIONAL INSTITUTE OF FRUIT TREE SCIENCE   (Japan)

^b NATIONAL INSTITUTE FOR AGRO ENVIRONMENTAL SCIENCES   (Japan)

Author keywords

Agrobacterium spp.; Apple; Colony PCR; Crown gall; Ti or Ri plasmid

Indexed keywords

AGROBACTERIUM; BACTERIA (MICROORGANISMS); MALUS X DOMESTICA; RHIZOBIUM;

EID: 12444331390     PISSN: 13452630     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10327-004-0133-8     Document Type: Article

References (17)

1. Bevan MW, Chilton M-D (1982) T-DNA of the Agrobacterium Ti and Ri plasmids. Ann Rev Genet 16:357-384
2. Brisbane PG, Kerr A (1983) Selective media for three biovars of Agrobacterium. J Appl Bacteriol 54:425-431
3. Guessow D, Clackson T (1989) Direct clone characterization from plaques and colonies by the polymerase chain reaction. Nucleic Acids Res 17:4000
4. Kawai Y, Sawada H, Koike H, Kondou K (1998) Detection of Agrobacterium tumefaciens by PCR. Ann Phytopathol Soc Jpn 64:377 (abstract in Japanese)
5. Kawai Y, Koike H, Kondou K (1999) Infection of crown gall from infected scions of Marubakaido (Malus prunifolia Bork) rootstocks. Ann Phytopathol Soc Jpn 65:366 (abstract in Japanese)
6. Louws FJ, Bell J, Medina-Mora CM, Smart CD, Opgenorth D, Ishimaru CA, Hausbeck MK, de Bruijn FJ, Fulbright DW (1998) rep-PCR-mediated genomic fingerprinting: a rapid and effective method to identify Clavibacter michiganensis. Phytopathology 88:862-868
7. Moore LW, Bouzar H, Burr T (2001) II. Gram-negative bacteria. In: Schaad NW, Jones JB, Chun W (eds) Laboratory guide for identification of plant pathogenic bacteria. 3rd edn. APS Press, St. Paul, MN, pp 17-35
8. Nekoduka S, Kawamura T, Nakatani F, Sasaki H, Onoda K (2001) Occurrence of crown gall on apple rootstock "JM" strain. Ann Rep Plant Prot North Japan 52:105-108 (in Japanese)
9. Perry KL, Kado CI (1982) Characteristics of Ti plasmids from broad-host-range and ecologically specific biotype 2 and 3 strains for Agrobacterium tumefaciens. J Bacteriol 151:343-350
10. Sandhu GS, Precup JW, Kline BC (1989) Rapid one step characterization of recombinant vectors by direct analysis of transformed Escherichia coli colonies. Biotechniques 7:689-690
11. Sawada H (2004) Rhizobium. In: Plant pathogenic bacteria: a reference guide. CABI Publishing, Division of CAB International, Wallingford (in press)
12. Sawada H, Tsuchiya K (2003) Taxonomy of the genus Agrobacterium. Jpn J Phytopathol 69:349-365 (in Japanese)
13. Sawada H, Ieki H, Matsuda I (1995) PCR detection of Ti and Ri plasmid from phytopathogenic Agrobacterium strains. Appl Environ Microbiol 61:828-831
14. Schaad NW (2001) I. Initial identification of common genera. In: Schaad NW, Jones JB, Chun W (eds) Laboratory guide for identification of plant pathogenic bacteria, 3rd edn. APS Press, St. Paul, MN, pp 1-16
15. Schaad NW, Cheong SS, Tamaki S, Hatziloukas E, Panopoulos J (1995) A combined biological and enzyme amplification (BIO-PCR) technique to detect Pseudomonas syringae pv. phaseolicola in bean seed extracts. Phytopathology 85:243-248
16. Schmitz FJ, Steiert M, Hofmann B, Verhoef J, Hadding U, Heinz HP, Kohrer K (1997) Devolopment of a multiplex-PCR for direct detection of the genes for enterotoxin B and C, and toxic shock syndrome toxin-1 in Staphylococcus aureus isolates. J Med Microbiol 47:335-340
17. Tsuchizaki N, Ishikawa J, Hotta K (2000) Colony PCR for rapid detection of antibiotic resistance genes in MRSA and enterococci. Jpn J Antibiot 53:422-429 (in Japanese)