Molecular identification of Sphingomonas sp. A1 alginate lyase (A1-IV′) as a member of novel polysaccharide lyase family 15 and implications in alginate lyase evolution

Journal of Bioscience and Bioengineering

Volumn 99, Issue 1, 2005, Pages 48-54

  Hashimoto, Wataru ^a   Miyake, Osamu ^a   Ochiai, Akihito ^a   Murata, Kousaku ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Alginate lyase; Evolution; Molecular diversity; Polysaccharide lyase family 15; Sphingomonas sp. A1

Indexed keywords

BACTERIA; CELLS; CYTOLOGY; ESCHERICHIA COLI; GENES; METABOLISM; POLYMERIZATION; POLYSACCHARIDES; STRAIN;

CYTOPLASM; GENES ENCODING; MOLECULAR IDENTIFICATION;

ENZYMES;

ALGINATE LYASE; ALGINIC ACID; GENOMIC DNA; LYASE; POLYSACCHARIDE LYASE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL CELL; DNA SEQUENCE; ENZYME ASSAY; ENZYME PURIFICATION; ESCHERICHIA COLI; MOLECULAR EVOLUTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; SPHINGOMONAS; WESTERN BLOTTING;

ESCHERICHIA COLI; SPHINGOMONAS SP.;

EID: 12444285375     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.99.48     Document Type: Article

References (22)

1. Gacesa, P.: Alginates. Carbohydr. Polym., 8, 161-182 (1988).
2. Wong, T. Y., Preston, L. A., and Schiller, N. L.: Alginate lyase: review of major sources and enzyme characteristics, structure-function analysis, biological roles, and applications. Annu. Rev. Microbiol., 54, 289-340 (2000).
3. Suda, K., Tanji, Y., Hori, K., and Unno, H.: Evidence for a novel Chlorella virus-encoded alginate lyase. FEMS Microbiol. Lett., 180, 45-53 (1999).
4. Sugimoto, I., Hiramatsu, S., Murakami, D., Fujie, M., Usami, S., and Yamada, T.: Algal-lytic activities encoded by Chlorella virus CVK2. Virology, 277, 119-126 (2000).
5. Shimizu, E., Ojima, T., and Nishita, K.: cDNA cloning of an alginate lyase from abalone, Haliotis discus hannai. Carbohydr. Res., 338, 2841-2852 (2003).
6. Murata, K., Inose, T., Hisano, T., Abe, S., Yonemoto, Y., Yamashita, T., Takagi, M., Sakaguchi, K., Kimura, A., and Imanaka, T.: Bacterial alginate lyase: enzymology, genetics and application. J. Ferment. Bioeng., 76, 427-437 (1993).
7. Hashimoto, W., Miyake, O., Momma, K., Kawai, S., and Murata, K.: Molecular identification of oligoalginate lyase of Sphingomonas sp. strain A1 as one of the enzymes required for complete depolymerization of alginate. J. Bacteriol., 182, 4572-4577 (2000).
8. Yonemoto, Y., Tanaka, H., Hisano, T., Sakaguchi, K., Abe, S., Yamashita, T., Kimura, A., and Murata, K.: Bacterial alginate lyase gene: nucleotide sequence and molecular route for the generation of alginate lyase species. J. Ferment. Bioeng., 75, 336-342 (1993).
9. Hashimoto, W., Okamoto, M., Misano, T., Momma, K., and Murata, K.: Sphingomonas sp. A1 lyase active on both poly-β-D-mannuronate and heteropolymeric regions in alginate. J. Ferment. Bioeng., 86, 236-238 (1998).
10. Yoon, H.-J., Hashimoto, W., Miyake, O., Okamoto, M., Mikami, B., and Murata, K.: Overexpression in Escherichia coli, purification, and characterization of Sphingomonas sp. A1 alginate lyases. Protein Expr. Purif., 19, 84-90 (2000).
11. Hisano, T., Nishimura, M., Yamashita, T., Sakaguchi, K., and Murata, K.: On the self-processing of bacterial alginate lyase. J. Ferment. Bioeng., 78, 109-110 (1994).
12. Miyake, O., Hashimoto, W., and Murata, K.: An exotype alginate lyase in Sphingomonas sp. A1: overexpression in Escherichia coli, purification, and characterization of alginate lyase IV (A1-IV). Protein Expr. Purif., 29, 33-41 (2003).
13. Momma, K., Okamoto, M., Mishima, Y., Mori, S., Hashimoto, W., and Murata, K.: A novel bacterial ATP-binding cassette transporter system that allows uptake of macromolecules. J. Bacteriol., 182, 3998-4004 (2000).
14. Miyake, O., Ochiai, A., Hashimoto, W., and Murata, K.: Origin and diversity of alginate lyases of families PL-5 and -7 in Sphingomonas sp. strain A1. J. Bacteriol., 186, 2891-2896 (2004).
15. Saito, H. and Miura, K.: Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim. Biophys. Acta, 72, 619-629 (1963).
16. Sambrook, J., Fritsch, E. F., and Maniatis, T.: Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York (1989).
17. Weissbach, A. and Hurwitz, J.: The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. J. Biol. Chem., 234, 705-709 (1959).
18. Conn, E. E., Stumpe, P. K., Bruening, G., and Doi, R. H.: Enzymes, p. 115-163. In Conn, E. E., Stumpe, P. K., Bruening, G., and Doi, R. H. (ed.), Outlines of biochemistry. John Wiley and Sons, New York (1987).
19. Bradford, M. M.: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254 (1976).
20. Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
21. Warren, L.: Thiobarbituric acid spray reagent for deoxy sugars and sialic acids. Nature, 186, 237 (1960).
22. Hashimoto, W., Suzuki, H., Yamamoto, K., and Kumagai, H.: Effect of site-directed mutations on processing and activity of γ- glutamyltranspeptidase of Escherichia coli K-12. J. Biochem., 118, 75-80 (1995).