Exercise training and supplementation with carnitine and antioxidants increases carnitine stores, triglyceride utilization, and endurance in exercising rats

Journal of Nutritional Science and Vitaminology

Volumn 50, Issue 5, 2004, Pages 335-343

  Kim, Eugene ^a   Park, Hyun ^b   Cha, Youn Soo ^a  

^a Chonbuk National University   (South Korea)

^b Kyung Hee University   (South Korea)

Author keywords

Antioxidants; Carnitine; CPT I; Endurance time; Exercise training; Lipids; Supplementation

Indexed keywords

ALPHA TOCOPHEROL; ANTIOXIDANT; ASCORBIC ACID; CARNITINE; CARNITINE PALMITOYLTRANSFERASE; HIGH DENSITY LIPOPROTEIN CHOLESTEROL; MELATONIN; TRIACYLGLYCEROL;

ANIMAL EXPERIMENT; ARTICLE; CHOLESTEROL BLOOD LEVEL; CONTROLLED STUDY; DIET SUPPLEMENTATION; ENDURANCE; EXERCISE; LIPID BLOOD LEVEL; MALE; NONHUMAN; RAT; STOMACH; TREADMILL; TRIACYLGLYCEROL BLOOD LEVEL;

ANIMALIA;

EID: 12444268266     PISSN: 03014800     EISSN: 18817742     Source Type: Journal    
DOI: 10.3177/jnsv.50.335     Document Type: Article

References (61)

1. Bieber LL. 1988. Carnitine. Annu Rev Biochem 57: 261-283.
2. Siliprandi N. 1985. Carnitine and its role in metabolism. Acta Med Rom 23: 237-250.
3. Long CS, Haller RG, Foster DW, McGarry JD. 1982. Kinetics of carnitine-dependent fatty acid oxidation: implication for human carnitine deficiency. Neurology 32: 663-666.
4. Brass EP, Hoppel CL, Hiatt WR. 1994. Effect of intravenous L-carnitine on carnitine homeostasis and fuel metabolism during exercise in humans. Clin Pharmacol Ther 55: 681-692.
5. Decombaz J, Deriaz O, Acheson K, Giavemder B, Jeguier E. 1993. Effect of L-carnitine on submaximal exercise metabolism after depletin on muscle glycogen. Med Sci Sports Exerc 25: 733-741.
6. Marconi C, Sassi G, Carpinelli A, Cerretelli P. 1985. Effects of L-carnitine loading on the aerobic and anaerobic performance of endurance athletes. Eur J Appl Physiol Occup Physiol 54: 131-135.
7. Greig C, Finch KM, Jones DA, Cooper M, Sargeant AJ, Forte CA. 1987. The effect of oral supplementaion with L-carnitine on maximum and submaximum exercise capacity. Eur J Appl Phys 56: 457-460.
8. Oyono-Enguelle S, Freund H, Ott C, Gartner M, Heitz A, Marbach J, Maccari F, Frey A, Bigot H, Bach AC. 1988. Prolonged submaximal exercise and L-carnitine in humans. Eur J Appl Phys 58: 53-61.
9. Jenkins RR. 1988. Free radical chemistry. Relationship to exercise. Sports Med 5: 156-170.
10. Leewenburgh C, Ji LL. 1995. Glutathione depletion in rested and exercised mice: Biochemical consequence and adaptation. Arch Biochem Biophys 316: 941-949.
11. Baskin CR, Hinchcliff KW, DiSilvestro RA, Reinhart GA, Hayek MG, Chew BP, Burr JR, Swenson RA. 2000. Effects of dietary antioxidant supplementation on oxidative damage and resistance to oxidative damage during prolonged exercise in sled dogs. Am J Vet Res 61: 886-891.
12. Schroder H, Navarro E, Tramullas A, Mora J, Galiano D. 2000. Nutrition antioxidant status and oxidative stress in professional basketball players: Effects of a three compound antioxidative supplement. Int J Sports Med 21: 146-150.
13. Decombaz JE, Roffect B, Bloemhard Y. 1987. L-Carnitine supplementation, caffeine and fuel oxidation in the exercising rat. Nutr Res 7: 923-933.
14. Lim K, Choi S, Cha Y-S, Sachan DS. 1997. Carnitine and caffeine administration increases fat oxidation and endurance performance in athletes [Abstract]. FASEB J 11: A375.
15. Short SH, Marquart LF. 1993. Sports nutrition fraud. New York State. J Med 93: 112-116.
16. Sen CK. 1995. Oxidants and antioxidants in exercise. J Appl Physiol 79: 675-686.
17. Sacheck JM, Blumberg JB. 2001. Role of vitamin E and oxidative stress in exercise. Nutrition 17: 809-814.
18. Tan Dx, Reiter RJ, Manchester LC, Yan MT, EL-Sawi M, Mayo JC, Kohen R, Allegra M, Hardeland R. 2002. Chemical and physical properties and potential mechanisms: Melatonin as a broad spectrum antioxidant and free radical scavenger. Curr Top Med Chem 2: 181-197.
19. Okatani Y, Wakatsuki A, Kaneda C. 2000. Melatonin increases activities of glutathione peroxidase and superoxide dismutase in fetal rat brain. J Pineal Res 28: 89-96.
20. Cederblad G, Lindstedt S. 1972. Excretion of L-carnitine in man. Clin Chim Acta 3: 117-123.
21. Sachan DS, Rhew TH, Ruark RA. 1984. Ameliorating effects of carnitine and its precursors on alcohol-induced fatty liver. Am J Clin Nutr 39: 499-502.
22. Bradford M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
23. Johnson D, Lardy H. 1967. Isolation of liver or kidney mitochondria. In: Methods in Enzymology. vol.10. (R.W. Estabrook. ed.). p 94-96, Academic Press, New York.
24. Guzman M, Castro J, Maquedano A. 1987. Ethanol feeding to rats reversibly decreases hepatic carnitine palmitoyltransferase activity and increases enzyme sensitivity to malonyl-CoA. Biochem Biophys Res Commun 149: 443-448.
25. Okamura M. 1980. An improved method for determination of L-ascorbic acid and L-dehydroascorbic acid in blood plasma. Clin Chimica Acta 103: 259-268.
26. Chomcznski P, Sacchi N. 1987. Single-step method of RNA isolation by acid fuanidinium thiocyanate-phenolchloroform extraction. Anal Biochem 162: 156-159.
27. Park JH, Lee HY, Roh SC, Kim HY, Yang YM. 2000. Screening of differentially expressed genes by desferrioxamine or feffic ammonium citrate treatment in HepG2 cells. J Biochem Mol Biol 33: 396-401.
28. Mynatt RL, Park EA, Thorngate PE, Das HK, Cook GA. 1994. Changes in carnitine palmitoyltransferase-1 mRNA abundance produced by hyperthyroidsm and hyphthyroidism parallel changes in activity. Biochem Biophys Res Commun 201: 932-937.
29. Fred Brouns, Ger J, van der Vusse. 1997. Utilization of lipids during exercise in human subjects: Metabolic and dietary constraints. Br J Nutr 79: 117-128.
30. Soop M, Bjorkman O, Cederblad G, Hegenfeldt L, Wahren J. 1988. Influence of carnitine supplementation on muscle substrate and carnitine metabolism during exercise. J Appl Physiol 64: 2394-2399.
31. Cha YS, Kim IS, Joo EJ. 1995. Comparison of body fat metabolism in middle-aged women depending upon swimming practice. Korean J Nutr 28: 397-405.
32. Weltman A, Matter S, Stanford BA. 1980. Caloric restriction and/or mild exercise: Effects on serum lipids and body composition. Am J Clin Nutr 33: 1002-1009.
33. Ji LL. 1995. Oxidative stress during exercise: Implication of antioxidant nutrients. Free Radical Biol Med 18: 1079-1086.
34. Maxwell SR, Jakeman P, Thomason H, Leguen C, Thorpe GH. 1993. Changes in plasma antioxidant status during eccentric exercise and the effect of vitamin supplementation. Free Radic Res Commun 19: 191-202.
35. Kanter MM, Nolte LA, Holloszy JO. 1993. Effects of an antioxidant vitamin mixture on lipid peroxidation at rest and postexercise. J Appl Physiol 74: 965-969.
36. Baskin CR, Hinchcliff KW, DiSilvestro RA, Reinhart GA, Hayek MG, Chew BP, Burr JR, Swenson RA. 2000. Effects of dietary antioxidant supplementation on oxidative damage and resistance to oxidative damage during prolonged exercise in sled dogs. Am J Vet Res 61: 886-891.
37. Jakeman P, Maxwell S. 1993. Effect of antioxidant vitamin supplementation on muscle function after eccentric exercise. Eur J Appl Physiol 67: 426-430.
38. Sen CK, Rankinen T, Vaisanen S, Rauramaa R. 1997. Oxidative stress after human exercise: Effects of N-acetylcycsteine supplementation. J Appl Physiol 76: 2570-2577.
39. Child RB, Wilkinson DM, Fallowfield JL. 1999. Resting serum antioxidant status is positively correlated with potential oxygen uptake in endurance trained runners. J Sports Med Phys Fitness 39: 282-284.
40. Bacurau RFP, Navarro F, Bassit RA, Meneguello MO, Santos RVT. 2003 Almeida ALR. Costa Rosa LFBP. Does exercise training interfere with the effects of L-carnitine supplementation? Nutrition 19: 337-341.
41. Hiatt WR, Wolfcl EE, Regensteiner JG, Ruff L, Brass EP. 1989. Carnitine metabolism during exercise in humans: dependent on skeletal muscle metabolic state. J Clin Invest 84: 1167-1173.
42. Shalin K. 1990. Muscle carnitine metabolism during incremental dynamic exercise in humans. Acta Physiol Scand 138: 259-262.
43. Brass EP, Hiatt WR. 1994. Carnitine metabolism during exercise. Life Sci 54: 1383-1393.
44. Decombaz JE, Roffect B, Bloemhard Y. 1990. Effect of L-carnitine and stimulated lipolysis on muscle substrate in the exercising rat. Experientia 46: 457-458.
45. Harris RC, Foster CVL, Hultman E. 1987. Acetylcarnitine formation during intense muscular contraction in humans. J Appl Physiol 63: 440-442.
46. Lennon DLF, Mance M. 1986. Interorgan cooperativity in carnitine metabolism in the trained rat. J Appl Physiol 60: 1659-1664.
47. McGarry JD, Brown NF. 1997. The mitochondrial carnitine palmitoyltransferase system. From concept to molecular analysis. Eur J Biochem 244: 1-14.
48. Guzman M, Castro J. 1988. Effects of endurance exercise on carnitine palmitoyltransferase-from rat heart, skeletal muscle and liver mitochondria. Biochem Biophys Acta 963: 562-565.
49. Cha YS, Kim HY, Soh JR, Oh SH. 2001. Effects of regular endurance or acute-exercise and rest on the levels of lipids, carnitine and carnitine palmitoyltransferase-I in rats. J Biochem Mol Biol 34: 434-439.
50. Guzman M, Castro J. 1988. Effects of endurance exercise on carnitine palmitoyltransferase I from rat heart, skeletal muscle and liver mitochondrial. Biochim Biophys Acta 961: 562-565.
51. Berthon PM, Howlett RA, Heigenhauser GJF, Spriet LL. 1998. Human skeletal muscle carnitine palmitoryltransferase I activity determined in isolated intact mitochondria. J Appl Physiol 85: 148-153.
52. Kiens B, Kristiansen S, Jensen P, Richter EA, Turcotte LP. 1997. Membrane associated fatty acid binding protein (FABPpm) in human skeletal muscle by endurance training. Biochem Biophys Res Commun 231: 463-465.
53. Spina RJ, Chi M-Y, Hopkins MG, Nemeth PM, Lowry OH, Holloszy JO. 1996. Mitochondrial enzyme increase in muscle in response to 7-10 days of cycle exercise. J Appl Physiol 80: 2250-2254.
54. Geng-Sheng Yu, Yi-Chun Lu, Tod Gulick. 1988. Co-regulation of tissue-specific alternative human carnitine palmitoyltransferase Iβ gene promoters by fatty acid enzyme substrate. J Biol Chem 278: 32901-32909.
55. McGarry JD, Leatherman GF, Foster DW. 1978. Carnitine palmitoyltrnasferase I. The site of inhibition of hepatic fatty acid oxidation by malonyl-CoA. J Biol Chem 253: 4128-4136.
56. McGarry JD, Mills SE, Long CS, Foster DW. 1983. Observation on the affinity for carnitine, and malonyl-CoA sensitivity, of carnitine palmitoyltransferase I in animal and human tissues. Biochem J 214: 21-28.
57. Prentki M, Vischer S, Glennon MC et al. 1992. Malonyl-CoA and long chain acyl-CoA esters as metabolic coupling factors in nutrient-induced insulin secretion. J Biol Chem 267: 5802-5810.
58. Saddik M, Gamble J, Witters LA, Lopaschuk GD. 1993. Acetyl-CoA carboxylase regulation of fatty acid oxidation in the heart. J Biol Chem 268: 25836-25845.
59. McMillin JB, Wang D, Witters LA, Buja LM. 1994. Kinetic properties of carnitine palmitoyltransferase I in cultured neonatal rat cardiac myocytes. Arch Biochem Biophys 312: 375-384.
60. Paned SV, Murthy MSR. 1994. Membrane proteins lose activity on freezing because of absorption: implications for activity and protein assays. Anal Biochem 222: 288-290.
61. Yu GS, Lu YC, Gulick T. 1998. Co-regulation of tissue-specific alternative himan carnitine palmitoyltransferase Iβ gene promoters by fatty acid enzyme substrate. J Biol Chem 273: 32901-32909.