Purification and cDNA cloning of a cecropin-like peptide from the great wax moth, Galleria mellonella

Molecules and Cells

Volumn 17, Issue 2, 2004, Pages 262-266

  Kim, Chong Han ^c   Lee, Joon Ha ^c   Kim, Iksoo ^a   Seo, Sook Jae ^b   Son, Seok Min ^c   Lee, Ki Young ^c   Lee, In Hee ^c  

^a National Institute of Animal Science   (South Korea)

^b Gyeongsang National University   (South Korea)

^c Hoseo University   (South Korea)

Author keywords

Antimicrobial peptide; cDNA; Cecropin; Galleria mellonella; Purification

Indexed keywords

CECROPIN; COMPLEMENTARY DNA; POLYPEPTIDE ANTIBIOTIC AGENT; SIGNAL PEPTIDE; ANTIINFECTIVE AGENT; INSECT PROTEIN; PEPTIDE;

5' UNTRANSLATED REGION; AMIDATION; AMINO TERMINAL SEQUENCE; ANTIBACTERIAL ACTIVITY; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DNA DETERMINATION; DNA PURIFICATION; DRUG PURIFICATION; DRUG SYNTHESIS; ESCHERICHIA COLI; HEMOLYMPH; IMMUNIZATION; LARVA; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR CLONING; MOLECULAR WEIGHT; MOTH; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN DEGRADATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; FAT BODY; GENETICS; HISTOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY;

GALLERIA MELLONELLA; LEPIDOPTERA;

AMINO ACID SEQUENCE; ANIMALS; ANTI-INFECTIVE AGENTS; BASE SEQUENCE; CLONING, MOLECULAR; FAT BODY; HEMOLYMPH; INSECT PROTEINS; LARVA; MOLECULAR SEQUENCE DATA; MOTHS; PEPTIDES; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 1242344146     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Beresford, P. J., Basinski-Gray, J. M., Chiu, J. K., Chadwick, J. S., and Aston, W. P. (1997) Characterization of hemolytic and cytotoxic Gallysins: a relationship with arylphorins. Dev. Comp. Immunol. 21, 253-266.
2. Boman, H. G. (1995) Peptide antibiotics and their role in innate immunity. Annu. Rev. Immunol. 13, 61-92.
3. Boman, H. G. and Hultmark, D. (1987) Cell-free immunity in insects. Annu. Rev. Microbiol. 41, 103-126.
4. Boman, H. C., Boman, I. A., Andreu, D., Li, Z. Q., Merrifield, R. B., Schlenstedt, G., and Zimmermann, R. (1989) Chemical synthesis and enzymic processing of precursor forms of cecropins A and B. J. Biol. Chem. 264, 5852-5860.
5. Bradbury, A. F. and Smyth, D. G. (1991) Peptide amidation. Trends Biochem. Sci. 16, 112-115.
6. Bulet, P., Hetru, C., Dimarcq, J. L., and Hoffmann, D. (1999) Antimicrobial peptides in insects; structure and function. Dev. Comp. Immunol. 23, 329-344.
7. Callaway, J. E., Lai, J., Haselbeck, B., Baltaian, M., Bonnesen, S. P., Weickmann, J., Wilcox, G., and Lei, S. P. (1993) Modification of the C terminus of cecropin is essential for broad-spectrum antimicrobial activity. Antimicrob. Agents Chemother 37, 1614-1619.
8. Dathe, M. and Wieprecht, T. (1999) Structural features of helical antimicrobial peptides: their potential to modulate activity on model membranes and biological cells. Biochim. Biophys. Acta 1462, 71-87.
9. Ganz, T., Selsted, M. E., Szklarek, D., Harwig, S. S., Daher, K. A., Bainton, D. F., and Lehrer, R. I. (1985) Defensins. Natural peptide antibiotics of human neutrophils. J. Clin. Invest. 76, 1427-1435.
10. Gudmundsson, G. H., Lidholm, D. A., Asling, B., Gan, R., and Boman, H. G. (1991) The cecropin locus. Cloning and expression of a gene cluster encoding three antibacterial peptides in Hyalophora cecropia. J. Biol. Chem. 266, 11510-11517.
11. Harwig, S. S., Chen, N. P., Park, A. S., and Lehrer, R. I. (1993) Purification of cysteine-rich bioactive peptides from leukocytes by continuous acid-urea-polyacrylamide gel electrophoresis. Anal. Biochem. 208, 382-386.
12. Hellers, M., Gunne, H., and Steiner, H. (1991) Expression of post-translational processing of preprocecropin A using a baculovirus vector. Eur. J. Biochem. 199, 435-439.
13. Kylsten, P., Samakovlis, C., and Hultmark, D. (1990) The cecropin locus in Drosophila; a compact gene cluster involved in the response to infection. EMBO J. 9, 217-224.
14. Lee, Y. S., Yun, E. K., Jang, W. S., Kim, I., Lee, J. H., Park, S. Y., Ryu, K. S., Seo, S. J., Kim, C. H., and Lee, I. H. (2004) Purification, cDNA cloning and expression of an insect defensin from the great wax moth, Galleria mellonella. Insect Mol. Biol. 13, 65-72.
15. Lowenberger, C., Charlet, M., Vizioli, J., Kamal, S., Richman, A., Christensen, B. M., and Bulet, P. (1999) Antimicrobial activity spectrum, cDNA cloning, and mRNA expression of a newly isolated member of the cecropin family from the mosquito vector Aedes aegypti. J. Biol. Chem. 274, 20092-20097.
16. Mak, P., Chmiel, D., and Gacek, G. J. (2001) Antibacterial peptides of the moth Galleria mellonella. Acta Biochim. Pol. 48, 1191-1195.
17. Park, S. S., Shin, S. W., Park, D. S., Oh, H. W., Boo, K. S., and Park, H. Y. (1997) Protein purification and cDNA cloning of a cecropin-like peptide from the larvae of fall webworm (Hyphantria cunea). Insect Biochem. Mol. Biol. 27, 711-720.
18. Schagger, H. and von Jagow, G. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 368-379.
19. Schuhmann, B., Seitz, V., Vilcinskas, A., and Podsiadlowski, L. (2003) Cloning and expression of gallerimycin, an antifungal peptide expressed in immune response of greater wax moth larvae, Galleria mellonella. Arch. Insect Biochem. Physiol. 53, 125-133.
20. Shin, S. Y., Lee, S. H., Yang, S. T., Park, E. J., Lee, D. G., Lee, M. K., Eom, S. H., Song, W. K., Kim, Y., Hahm, K. S., and Kim, J. I. (2001) Antibacterial, antitumor and hemolytic activities of alpha-helical antibiotic peptide, P18 and its analogs. J. Pept. Res. 58, 504-514.
21. Steinberg, D. A. and Lehrer, R. I. (1997) Designer assays for antimicrobial peptides. Disputing the "one-size-fits-all" theory. Methods Mol. Biol. 78, 169-186.
22. Yamano, Y., Matsumoto, M., Inoue, K., Kawabata, T., and Morishima, I. (1994) Cloning of cDNAs for cecropins A and B, and expression of the genes in the silkworm. Bombyx mori. Biosci. Biotechnol. Biochem. 58, 1476-1478.
23. Yu, K. H., Kim, K. N., Lee, J. H., Lee, H. S., Kim, S. H., Cho, K. Y., Nam, M. H., and Lee, I. H. (2002) Comparative study on characteristics of lysozymes from the hemolymph of three lepidopteran larvae, Galleria mellonella, Bombyx mori, Agrius convolvuli. Dev. Comp. Immunol. 26, 707-713.