A simple, rapid, and effective method for the extraction of Mycobacterium paratuberculosis DNA from fecal samples for polymerase chain reaction

Journal of Veterinary Diagnostic Investigation

Volumn 16, Issue 1, 2004, Pages 22-30

  Stabel, J R ^a   Bosworth, T L ^a   Kirkbride, T A ^b   Forde, R L ^b   Whitlock, R H ^c  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b Rocky Mt Reg Anim Hlth Lab ^*  (United States)

^c UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMALIA; BACTERIA (MICROORGANISMS); BOS TAURUS; MYCOBACTERIUM; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS; MYCOBACTERIUM PARATUBERCULOSIS;

EID: 1242277811     PISSN: 10406387     EISSN: None     Source Type: Journal    
DOI: 10.1177/104063870401600105     Document Type: Article

References (17)

1. Abbas B, Riemann HP, Hird DW: 1983, Diagnosis of Johne's disease (paratuberculosis) in northern California cattle and a note on its economic significance. Calif Vet 37:16.
2. Bendixen PH: 1978, Immunologic reactions caused by infections with Mycobacterium paratuberculosis. A review. Nord Vet Med 30:163-168.
3. Benedictus G, Dijkhuizen AA, Stelwagen J: 1987, Economic losses due to paratuberculosis in dairy cattle. Vet Rec 121:142-146.
4. Cocito C, Gilot P, Coene M, et al.: 1994, Paratuberculosis. Clin Microbiol Rev 7:328-345.
5. Collins DM, Stephens DM, de Lisle GW: 1993, Comparison of polymerase chain reaction tests and fecal culture for detecting Mycobacterium paratuberculosis in bovine feces. Vet Microbiol 36:289-299.
6. Ellingson JL, Bolin CA, Stabel JR: 1998, Identification of a gene unique to Mycobacterium avium subsp. paratuberculosis and application to diagnosis of paratuberculosis. Mol Cell Probes 12:133-142.
7. Fang Y, Wu W, Pepper JL, et al.: 2002, Comparison of real-time, quantitative PCR with molecular beacons to nested PCR and culture methods for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples. J Clin Microbiol 40:287-291.
8. Garrido JM, Cortabarria N, Oguiza JA, et al.: 2000, Use of a PCR method on fecal samples for diagnosis of sheep paratuberculosis. Vet Microbiol 77:379-386.
9. Johnson-Ifearulundu YJ, Kaneene JB: 1997, Epidemiology and economic impact of subclinical Johne's disease: a review. Vet Bull 67:437-447.
10. Marsh IB, Whittington RJ: 2001, Progress towards a rapid polymerase chain reaction diagnostic test for the identification of Mycobacterium avium subsp. paratuberculosis in faeces. Mol Cell Probes 15:105-118.
11. Mason O, Marsh IB, Whittington RJ: 2001, Comparison of immunomagnetic bead separation-polymerase chain reaction and faecal culture for the detection of Mycobacterium avium subsp. paratuberculosis in sheep faeces. Aust Vet J 79:497-500.
12. Sanderson JD, Moss MT, Tizard ML, et al.: 1992, Mycobacterium paratuberculosis DNA in Crohn's disease tissue. Gut 33: 890-896.
13. Stabel JR: 1996, An improved method for cultivation of Mycobacterium paratuberculosis from bovine fecal samples and comparison to three other methods. J Vet Diagn Invest 9:375-380.
14. Stabel JR: 2000, Transitions in immune responses to Mycobacterium paratuberculosis. Vet Microbiol 77:465-473.
15. Vary PH, Andersen PR, Green E, et al.: 1990, Use of a highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne's disease. J Clin Microbiol 28:933-937.
16. Wells SJ, Ott SL, Seitzinger AH: 1998, Key health issues for dairy cattle - new and old. J Dairy Sci 81:3029-3035.
17. Whipple DL, Kapke PA, Andersen PR: 1992, Comparison of a commercial DNA probe test and three cultivation procedures for detection of Mycobacterium paratuberculosis in bovine feces. J Vet Diagn Invest 4:23-27.