R174 of Escherichia coli FtsZ is involved in membrane interaction and protofilament bundling, and is essential for cell division

Molecular Microbiology

Volumn 51, Issue 3, 2004, Pages 645-657

  Koppelman, Cecile Marie ^a,c   Aarsman, Mirjam E G ^a   Postmus, Jarne ^a   Pas, Evelien ^a   Muijsers, Anton O ^a   Scheffers, Dirk Jan ^b,d   Nanninga, Nanne ^a   Den Blaauwen, Tanneke ^a  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b UNIVERSITY OF GRONINGEN   (Netherlands)

^c UTRECHT UNIVERSITY   (Netherlands)

^d UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; ASPARTIC ACID; CALCIUM CHLORIDE; FTSZ PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; TRYPSIN;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; BINDING AFFINITY; CELL DIVISION; CELL MEMBRANE; CELL ULTRASTRUCTURE; CONTROLLED STUDY; ESCHERICHIA COLI; HYDROPHOBICITY; IN VITRO STUDY; MEMBRANE VESICLE; MUTATION; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SEQUENCE ANALYSIS; STRUCTURE ANALYSIS; TEMPERATURE DEPENDENCE; WILD TYPE;

AMINO ACID SEQUENCE; ARGININE; CELL DIVISION; CELL MEMBRANE; CYTOSKELETON; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC COMPLEMENTATION TEST; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; POLYMERS; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; TEMPERATURE; TRYPSIN;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 1242275409     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2003.03876.x     Document Type: Article

References (35)

1. Addinall, S.G., Cao, C., and Lutkenhaus, J. (1997) Temperature shift experiments with an FtsZ84(Ts) strain reveal rapid dynamics of FtsZ localization and indicate that the Z ring is required throughout septation and cannot reoccupy division sites once constriction has initiated. J Bacteriol 179: 4277-4284.
2. Bi, E., and Lutkenhaus, J. (1991) FtsZ ring structure associated with division in Escherichia coli. Nature 354: 161-164.
3. Buddelmeijer, N., Judson, N., Boyd, D., Mekalanos, J.J., and Beckwith, J. (2002) YgbQ, a cell division protein in Escherichia coli and Vibrio cholerae, localizes in codependent fashion with FtsL to the division site. Proc Natl Acad Sci USA 99: 6316-6321.
4. Chen, J.C., and Beckwith, J. (2001) FtsQ, FtsL and FtsI require FtsK but not FtsN, for co-localization with FtsZ during Escherichia coli cell division. Mol Microbiol 42: 395-413.
5. Crameri, A., Whitehorn, E.A., Tata, E., and Stemmer, W.P.C. (1996) Improved green fluorescent protein by molecular evolution using DNA shuffling. Nature Biotechn 14: 315-319.
6. De Vrije, T., Tommassen, J., and de Kruijff, B. (1987) Optimal posttranslocational of the precursor of PhoE protein across Escherichia coli membrane vesicles requires both ATP and the protonmotive force. Biochim Biophys Acta 900: 63-72.
7. Den Blaauwen, T., Buddelmeijer, N., Aarsman, M.E.G., Hameete, C.M., and Nanninga, N. (1999) Timing of the FtsZ assembly in Escherichia coli. J Bacteriol 181: 5167-5175.
8. Fishov, I., Zaritsky, A., and Grover, N.B. (1995) On microbial states of growth. Mol Microbiol 15: 789-794.
9. Garrido, T., Sánchez, M., Palcios, P., Aldea, M., and Vicente, M. (1993) Transcription of ftsZ oscillates during the cell cycle of Escherichia coli. EMBO J 12: 3957-3965.
10. Hale, C.A., Rhee, A.C., and de Boer, P.A.J. (2000) ZipA-induced bundling of FtsZ polymers mediated by an interaction between C-terminal domains. J Bacteriol 182: 5153-5166.
11. Koradi, R., Billeter, M., and Wüthrich, K. (1996) MOLMOL: a program for display and analysis of macromolecular structures. J Mol Graphics 14: 51-55.
12. Löwe, J., and Amos, L.A. (1998) Crystal structure of the bacterial cell-division protein FtsZ. Nature 391: 203-206.
13. 2+-induced FtsZ sheets. EMBO J 18: 2364-2371.
14. Lu, C., Stricker, J., and Erickson, H.P. (2001) Site specific mutations of FtsZ-effects on GTPase and in vitro assembly. BMC Microbiol 1: 7.
15. Ma, X., and Margolin, W. (1999) Genetic and functional analysis of the conserved C-terminal core domain of Escherichia coli FtsZ. J Bacteriol 181: 7531-7544.
16. Ma, X., Ehrhardt, D.W., and Margolin, W. (1996) Colocalization of cell division proteins FtsZ and FtsA to cytoskeletal structures in living Escherichia coli cells by using green fluorescent protein. Proc Natl Acad Sci USA 93: 12998-13003.
17. Mercer, K.L., and Weiss, D.S. (2002) The Escherichia coli cell division protein FtsW is required to recruit its cognate transpeptidase, FtsI (PBP3), to the division site. J Bacteriol 184: 904-912.
18. Mukherjee, A., and Lutkenhaus, J. (1998) Purification, assembly, and localization of FtsZ. Methods Enzymol 298: 296-305.
19. Mukherjee, A., and Lutkenhaus, J. (1999) Analysis of FtsZ assembly by light scattering and determination of the role of divalent metal cations. J Bacteriol 181: 823-832.
20. Nanninga, N. (1998) Morphogenesis of Escherichia coli. Microbiol Mol Biol Rev 62: 110-129.
21. Nogales, E., Downing, K.H., Amos, L.A., and Lowe, J. (1998) Tubulin and FtsZ form a distinct family of GTPases. Nature Struct Biol 5: 451-458.
22. Osborn, M.J., and Munson, R. (1979) Separation of the inner (cytoplasmic) and outer membranes of gram-negative bacteria. Methods Enzymol 56: 372-378.
23. Rothfield, L., Justice, S., and Garcia-Lara, J. (1999) Bacterial cell division. Annu Rev Genet 33: 423-448.
24. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
25. Scheffers, D.-J., Den Blaauwen, T., and Driessen, A.J.M. (2000) Non-hydrolyzable GTP-γ-S stabilizes the FtsZ polymer in a GDP-bound state. Mol Microbiol 35: 1211-1219.
26. Stricker, J., Maddox, P., Salmon, E.D., and Erickson, H.P. (2002) Rapid assembly dynamics of the Escherichia coli FtsZ-ring demonstrated by fluorescence recovery after photobleaching. Proc Natl Acad Sci USA 99: 3171-3175.
27. Sun, Q., and Margolin, W. (1998) FtsZ dynamics during the division cycle of live Escherichia coli cells. J Bacteriol 180: 2050-2056.
28. Taschner, P.E.M., Huls, P.G., Pas, E., and Woldringh, C.L. (1988) Division behaviour and shape changes in isogenic ftsZ, ftsQ, ftsA, pbpB, and ftsE cell division mutants of Escherichia coli during temperature shift experiments. J Bacteriol 170: 1533-1540.
29. Van Helvoort, J.M.L.M., and Woldringh, C.L. (1994) Nucleoid partitioning in Escherichia coli during steady-state growth and upon recovery from chloramphenicol treatment. Mol Microbiol 13: 577-583.
30. Voskuil, J.L.A., Westerbeek, C.A.M., Wu, C., Kolk, A.H.J., and Nanninga, N. (1994) Epitope mapping of Escherichia coli cell division protein FtsZ with monoclonal antibodies. J Bacteriol 176: 1886-1893.
31. Wang, L., and Lutkenhaus, J. (1998) FtsK is an essential cell division protein that is localized to the septum and induced as part of the SOS response. Mol Microbiol 29: 731-740.
32. Wang, Y., Jones, B.D., and Brun, Y.V. (2001) A set of ftsZ mutants blocked at different stages of cell division in Caulobacter. Mol Microbiol 40: 347-360.
33. Yi, Q.-M., and Lutkenhaus, J. (1985) The nucleotide sequence of the essential cell-division gene ftsZ of Escherichia coli. Gene 36: 241-247.
34. Yim, L., and Vicente, M. (1998) Submitted to EMBL, Genbank, DDBJ databases. Accession number U29400, Prot-id AAC24467.1.
35. 2+-mediated GTP-dependent dynamic assembly of bacterial cell division protein FtsZ into asters and polymer networks in vitro. EMBO J 16: 5455-5463.