Trapping radioactive carbon dioxide during cellular metabolic assays under standard culture conditions: Description of a unique gas-capturing device

Journal of Biochemical and Biophysical Methods

Volumn 58, Issue 2, 2004, Pages 119-124

  Krieg, Rene C ^a   Liotta, Lance A ^a   Petricoin III, Emanuel F ^b   Herrmann, Paul C ^a,b  

^a NATIONAL CANCER INSTITUTE   (United States)

^b CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

Carbon dioxide; Metabolism; Physiological; Radioactivity

Indexed keywords

CARBON DIOXIDE;

ARTICLE; CARBON DIOXIDE CAPTURE DEVICE; CELL CULTURE; CELL CULTURE EQUIPMENT; CELL GROWTH; CELL METABOLISM; DEVICE; PRIORITY JOURNAL;

EID: 1242269895     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbbm.2003.10.002     Document Type: Article

References (8)

1. Beckner M.E., Stracke M.L., Liotta L.A., Schiffmann E. Glycolysis as primary energy source in tumor cell chemotaxis. J. Natl. Cancer Inst. 82:1990;1836-1840.
2. Chakravarty N. Glucose metabolism in rat mast cells. Stimulation of the pentose phosphate pathway by compound 48/80. Agents Actions. 16:1985;133-137.
3. Bostwick J.R., Le W.D. A tyrosine hydroxylase assay in microwells using coupled nonenzymatic decarboxylation of dopa. Anal. Biochem. 192:1991;125-130.
4. 2 for liquid scintillation counting. Anal. Biochem. 91:1978;199-208.
5. 2 collection. Anal. Biochem. 253:1997;190-195.
6. Song M.K., Levin S.R. Metabolism of glucose by rat pancreatic islets and kidney: comparison of membrane preparations with intact tissue. Pancreas. 24:2002;205-209.
7. 2-capture assay for evaluation of substrate oxidation rates of cells in culture. BioTechniques. 24:1998;803-808.
8. Phillips J.L., Hayward S.W., Wang Y., Vasselli J., Pavlovich C., Padilla-Nash H.et al. The consequences of chromosomal aneuploidy on gene expression profiles in a cell line model for prostate carcinogenesis. Cancer Res. 61:2001;8143-8149.