Fusion expression of Helicobacter pylori neutrophil-activating protein in E. coli

World Journal of Gastroenterology

Volumn 11, Issue 3, 2005, Pages 454-456

  Kang, Qiao Zhen ^a   Duan, Guang Cai ^a   Fan, Qing Tang ^a   Xi, Yuan Lin ^a  

^a ZHENGZHOU UNIVERSITY   (China)

Author keywords

E.coli; Helicobacter pylori; Maltose binding protein; Neutrophill activating protein

Indexed keywords

HELICOBACTER PYLORI VACCINE; HYBRID PROTEIN; INTERLEUKIN 8; MALTOSE BINDING PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION; HELICOBACTER PYLORI; IMMUNOGENICITY; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; LEUKOCYTE ACTIVATION; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; WESTERN BLOTTING;

EID: 12344315785     PISSN: 10079327     EISSN: None     Source Type: Journal    
DOI: 10.3748/wjg.v11.i3.454     Document Type: Article

References (13)

1. Dundon WG, Nishioka H, Polenghi A, Papinutto E, Zanotti G, Montemurro P, Del GG, Rappuoli R, Montecucco C. The neutrophil-activating protein of Helicobacter pylori. Int J Med Microbiol 2002; 291: 545-550
2. Nishioka H, Baesso I, Semenzato G, Trentin L, Rappuoli R, Del Giudice G, Montecucco C. The neutrophil-activating protein of Helicobacter pylori (HP-NAP) activates the MAPK pathway in human neutrophils. Eur J Immunol 2003; 33: 840-849
3. Goodwin CS. Helicobacter pylori gastritis, peptic ulcer, and gastric cancer: Clinical and molecular aspects. Clin Infect Dis 1997; 25: 1017-1019
4. Bayerdorffer E, Lehn N Hatz R, Mannes GA, Oertel H, Sauerbruch T, Stolte M. Difference in expression of Helicobacter pylori gastritis in antrum and body. Gastroenterology 1992; 102: 1575-1582
5. Montecucco C, de Bernard M. Molecular and cellular mechanisms of action of the vacuolating cytotoxin (VacA) and neutrophil-activating protein (HP-NAP) virulence factors of Helicobacter pylori. Microbes Infect 2003; 5: 715-721
6. Namavar F, Sparrius M, Veerman EC, Appelmelk BJ, Vandenbroucke-Grauls CM. Neutrophil-activating protein mediates adhesion of Helicobacter pylori to sulfated carbohydrates on high-molecular-weight salivary mucin. Infect Immun 1998; 66: 444-447
7. Shimoyama T, Fukuda S, Liu Q, Nakaji S, Fukuda Y, Sugawara K. Helicobacter pylori water soluble surface proteins prime human neutrophils for enhanced production of reactive oxygen species and stimulate chemokine production. J Clin Pathol 2003; 56: 348-351
8. Phadnis SH, Parlow MH, Levy M, Ilver D, Caulkins CM, Connors JB, Dunn BE. Surface localization of Helicobacter pylori urease and a heat shock protein homolog requires bacterial autolysis. Infect Immun 1996; 64: 905-912
9. Satin B, Del Giudice G, Della Bianca V, Dusi S, Laudanna C, Tonello F, Kelleher D, Rappuoli R, Montecucco C, Rossi F. The neutrophil-activating protein (HP-NAP) of Helicobacter pylori is a protective antigen and a major virulence factor. J Exp Med 2000; 191: 1467-1476
10. Sambrook J, Fritsch EF, Maniatis T. MOLECULAR CLONING: A Laboratory Manual. 2nded. Cold Spring Harbor Laboratory Press 1989: 888-897
11. Bai Y, Li LR, Wang JD, Chen Y, Jin JF, Zhang ZS, Zhou DY, Zhang YL. Expression of Helicobacter pylori Hsp60 protein and its immunogenicity. World J Gastroenterol 2003; 9: 2711-2714
12. Evans DJ Jr, Evans DG. Helicobacter pylori CagA: Analysis of sequence diversity in relation to phosphorylation motifs and implications for the role of CagA as a virulence factor. Helicobacter 2001; 6: 187-198
13. Chakravarti DN, Fiske MJ, Fletcher LD, Zagursky RJ. Application of genomics and proteomics for identification of bacterial gene products as potential vaccine candidates. Vaccine 2000; 19 601-612