Interaction of the 20 kDa and 63 kDa fragments of anthrax protective antigen: Kinetics and thermodynamics

Biochemistry

Volumn 44, Issue 3, 2005, Pages 1047-1053

  Christensen, Kenneth A ^a   Krantz, Bryan A ^a   Melnyk, Roman A ^a   Collier, R John ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; DISSOCIATION; ENERGY TRANSFER; FLUORESCENCE; ISOMERIZATION; REACTION KINETICS; THERMODYNAMICS; TOXIC MATERIALS;

MOIETIES; OLIGOMERIZATION; PROTECTIVE ANTIGENS (PA); SPECIES;

ANTIGENS;

ANTHRAX TOXIN; BACTERIAL ANTIGEN; CELL SURFACE RECEPTOR; FURIN; PROTEINASE; TRYPSIN;

AMINO TERMINAL SEQUENCE; ANTIGEN BINDING; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL SURFACE; CYTOSOL; DISSOCIATION; EQUILIBRIUM CONSTANT; FLUORESCENCE RESONANCE ENERGY TRANSFER; ISOMERIZATION; KINETICS; MAMMAL; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; THERMODYNAMICS;

AMINO ACID SEQUENCE; ANTIGENS, BACTERIAL; BACILLUS ANTHRACIS; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; HYDROLYSIS; KINETICS; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; SEQUENCE HOMOLOGY, AMINO ACID; THERMODYNAMICS;

ANTHRAX; BACTERIA (MICROORGANISMS); MAMMALIA; POSIBACTERIA;

EID: 12344270920     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi047791s     Document Type: Article

References (27)

1. Smith, H., and Stoner, H. B. (1967) Anthrax toxic complex, Fed. Proc. 26, 1554-1557.
2. Smith, H. (2000) Discovery of the anthrax toxin: the beginning of in vivo studies on pathogenic bacteria, Trends Microbiol. 8, 199-200.
3. Duesbery, N. S., Resau, J., Webb, C. P., Koochekpour, S., Koo, H. M., Leppla, S. H., and Vande Woude, G. F. (2001) Suppression of ras-mediated transformation and inhibition of tumor growth and angiogenesis by anthrax lethal factor, a proteolytic inhibitor of multiple MEK pathways, Proc. Natl. Acad. Sci. U.S.A. 98, 4089-4094.
4. Pellizzari, R., Guidi-Rontani, C., Vitale, G., Mock, M., and Montecucco, C. (2000) Lethal factor of Bacillus anthracis cleaves the N-terminus of MAPKKs: analysis of the intracellular consequences in macrophages, Int. J. Med. Microbiol. 290, 421-427.
5. Vitale, G., Pellizzari, R., Recchi, C., Napolitani, G., Mock, M., and Montecucco, C. (1998) Anthrax lethal factor cleaves the N-terminus of MAPKKs and induces tyrosine/threonine phosphorylation of MAPKs in cultured macrophages, Biochem. Biophys. Res. Commun. 248, 706-711.
6. Bradley, K. A., Mogridge, J., Mourez, M., Collier, R. J., and Young, J. A. (2001) Identification of the cellular receptor for anthrax toxin, Nature. 414, 225-229.
7. Scobie, H. M., Rainey, G. J., Bradley, K. A., and Young, J. A. (2003) Human capillary morphogenesis protein 2 functions as an anthrax toxin receptor, Proc. Natl. Acad. Sci. 100, 5170-5174.
8. Klimpel, K. R., Molloy, S. S., Thomas, G., and Leppla, S. H. (1992) Anthrax toxin protective antigen is activated by a cell surface protease with the sequence specificity and catalytic properties of furin, Proc. Natl. Acad. Sci. 89, 10277-10281.
9. Petosa, C., Collier, R. J., Klimpel, K. R., Leppla, S. H., and Liddington, R. C. (1997) Crystal structure of the anthrax toxin protective antigen, Nature. 385, 833-838.
10. Elliott, J. L., Mogridge, J., and Collier, R. J. (2000) A quantitative study of the interactions of Bacillus anthracis edema factor and lethal factor with activated protective antigen, Biochemistry 39, 6706-6713.
11. Mogridge, J., Cunningham, K., and Collier, R. J. (2002) Stoichiometry of anthrax toxin complexes, Biochemistry 41, 1079-1082.
12. Abrami, L., Liu, S., Cosson, P., Leppla, S. H., and van der Goot, F. G. (2003) Anthrax toxin triggers endocytosis of its receptor via a lipid raft-mediated clathrin-dependent process, J. Cell Biol. 160, 321-328.
13. Milne, J. C., and Collier, R. J. (1993) pH-dependent permeabilization of the plasma membrane of mammalian cells by anthrax protective antigen, Mol. Microbiol. 10, 647-653.
14. Blaustein, R. O., Koehler, T. M., Collier, R. J., and Finkelstein, A. (1989) Anthrax toxin: channel-forming activity of protective antigen in planar phospholipid bilayers, Proc. Natl. Acad. Sci. U.S.A. 86, 2209-2213.
15. O'Brien, J., Friedlander, A., Dreier, T., Ezzell, J., and Leppla, S. (1985) Effects of anthrax toxin components on human neutrophils, Infect. Immun. 47, 306-310.
16. Bernard, A., and Payton, M. (1995) in Current Protocols in Protein Science (Coligan, J. E., Dunn, B. M., Plough, H. L., Speicher, D. W., and Wingfield, P. T., Eds.) pp 1-18, John Wiley & Sons, Inc., New York.
17. Miller, C. J., Elliott, J. L., and Collier, R. J. (1999) Anthrax protective antigen: prepore-to-pore conversion, Biochemistry 38, 10432-10441.
18. Lacy, D. B., Mourez, M., Fouassier, A., and Collier, R. J. (2002) Mapping the anthrax protective antigen binding site on the lethal and edema factors, J. Biol. Chem. 277, 3006-3010.
19. Wigelsworth, D. J., Krantz, B. A., Christensen, K. A., Lacy, D. B., Juris, S. J., and Collier, R. J. (2004) Binding Stoichiometry and Kinetics of the Inteaction of a Human Anthrax Toxin Receptor, CMG2, with Protective Antigen, J. Biol. Chem. 279, 23349-23356.
20. Lakowicz, J. R. (1999) Principles of Fluorescence Spectroscopy, 2nd ed., Kluwer Academic/Plenum Publishers, New York.
21. Mourez, M., Yan, M., Lacy, D. B., Dillon, L., Bentsen, L., Marpoe, A., Maurin, C., Hotze, E., Wigelsworth, D., Pimental, R. A., Ballard, J. D., Collier, R. J., and Tweten, R. K. (2003) Mapping dominant-negative mutations of anthrax protective antigen by scanning mutagenesis, Proc. Natl. Acad. Sci. 100, 13803-13808.
22. Wu P. G., and Brand L. (1994) Resonance Energy Transfer: Methods and Applications, Anal. Biochem. 218, 1-13.
23. Novak, J. M., Stein, M. P., Little, S. F., Leppla, S. H., and Friedlander, A. M. (1992) Functional characterization of protease-treated Bacillus anthracis protective antigen, J. Biol. Chem. 267, 17186-17193.
24. Molloy, S. S., Bresnahan, P. A., Leppla, S. H., Klimpel, K. R., and Thomas, G. (1992) Human furin is a calcium-dependent serine endoprotease that recognizes the sequence Arg-X-X-Arg and efficiently cleaves anthrax toxin protective antigen, J. Biol. Chem. 267, 16396-16402.
25. Beckingham, J. A., Bottomley, S. P., Hinton, R., Sutton, B. J., and Gore, M. G. (1999) Interactions between a single immunoglobulin-binding domain of protein L from Peptostreptococcus magnus and a human κ light chain, Biochem. J. 340, 193-199.
26. Wallis, R., Moore, G. R., James, R., and Kleanthous, C. (1995) Protein-Protein Interactions in Colicin E9 DNase-Immunity Protein Complexes. 1. Diffusion-Controlled Association and Femtomolar Binding for the Cognate Complex, Biochemistry 34, 13743-13750.
27. Joshi, R. V., Zarutskie, J. A., and Stern, L. J. (2000) A Three-Step Kinetic Mechanism for Peptide Binding to MHC Class II Proteins, Biochemistry 39, 3751-3762.