Proteomic analysis of rat brain tissue: Comparison of protocols for two-dimensional gel electrophoresis analysis based on different solubilizing agents

Electrophoresis

Volumn 23, Issue 24, 2002, Pages 4132-4141

  Carboni, Lucia ^a   Piubelli, Chiara ^b   Righetti, Pier Giorgio ^b   Jansson, Birger ^a   Domenici, Enrico ^a  

^a GSK   (Italy)

^b UNIVERSITY OF VERONA   (Italy)

Author keywords

Immunoblot; Nonidet P 40; Rat brain; Sypro Ruby; Two dimensional gel electrophoresis

Indexed keywords

BIOLOGICAL MEMBRANES; BRAIN; ELECTROPHORESIS; HOMOGENIZATION METHOD; RATS; SOAPS (DETERGENTS); TISSUE;

DELIPIDATION; GEL ELECTROPHORESIS ANALYSIS; IMMUNOBLOTS; MEMBRANE PROTEINS; NONIDET P-40; PROTEOMIC ANALYSIS; RAT BRAIN TISSUE; RATS BRAIN; SYPRO RUBY; TWO-DIMENSIONAL GEL ELECTROPHORESIS;

ANTIBODIES;

3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 1 PROPANESULFONIC ACID; AMPHOLYTE; MEMBRANE PROTEIN; PROTEINASE INHIBITOR; SOLUBILIZER; TRITON X 114;

ANIMAL TISSUE; ARTICLE; BRAIN HOMOGENATE; BRAIN MAPPING; CONTROLLED STUDY; EXTRACTION; IMAGE ANALYSIS; IMMUNOBLOTTING; MALE; MOLECULAR WEIGHT; NONHUMAN; PROTEIN ANALYSIS; PROTEOMICS; QUALITATIVE ANALYSIS; RAT; TWO DIMENSIONAL GEL ELECTROPHORESIS; WESTERN BLOTTING;

EID: 12244289683     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.200290031     Document Type: Article

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