Optimization of spinning disk confocal microscopy: Synchronization with the ultra-sensitive EMCCD

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 5, Issue 13, 2004, Pages 65-76

  Chong, F K ^a   Coates, Colin G ^a   Denvir, Donal J ^a   McHale, Noel ^b   Thornbury, Keith ^b   Hollywood, Mark ^b  

^a OXFORD INSTRUMENTS PLASMA TECHNOLOGY   (United Kingdom)

^b QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

Author keywords

CCD; Confocal Microscopy; Confocal spinning Disk; Electron Multiplying CCD; EMCCD; Live Cell Microscopy; Nipkow; Single Photon Sensitivity

Indexed keywords

CONFOCAL MICROSCOPY; CONFOCAL SPINNING DISK; ELECTRON MULTIPLYING CHARGE COUPLED DEVICE (EMCCD); LIVE CELL MICROSCOPY; NIPKOW; SINGLE PHOTON SENSITIVITY;

CELLS; DYES; FLUORESCENCE; MEDICAL IMAGING; MICROSCOPIC EXAMINATION; OPTICAL MICROSCOPY; OPTICAL SENSORS; PHOTONS; SYNCHRONIZATION; VIDEO CAMERAS;

CHARGE COUPLED DEVICES;

EID: 12144284962     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.529220     Document Type: Conference Paper

References (9)

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2. C. D. Mackay et al, "Sub-Electron Read Noise at MHz Pixel Rates", http://www.marconitech.com/ccds/13vision/technology.php.
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4. D. J. Denvir and Colin G. Coates, "Electron Multiplying CCD Technology: Application to Ultrasensitive Detection of Biomolecules", Proc. of SPIE Vol 4626 pp502-512, Jan 2002.
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7. M. A. Hollywood, L. Johnston, N. G. McHale and K. D. Thornbury, "Spontaneous Calcium Transients in Isolated Interstitial Cells from the Rabbit Urethra", J. Physiol Vol 548, 2003 (in press).
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