Performance of dual axes confocal microscope for in vivo molecular and cellular imaging

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 5, Issue 13, 2004, Pages 35-46

  Kino, Gordon ^a   Wang, Thomas ^a   Contag, Chris ^a   Mandella, Michael ^b   Chan, Ning ^b  

^a STANFORD UNIVERSITY   (United States)

^b Optical Biopsy Technologies Inc   (United States)

Author keywords

Confocal; Fluorescence; Imaging; In vivo; MEMS; Microscopy; Reflectance

Indexed keywords

CONFOCAL MICROSCOPY; IN VIVO IMAGING; MEMS; WORKING DISTANCE (WD);

BIOPSY; FLUORESCENCE; HISTOLOGY; MATHEMATICAL MODELS; MICROELECTROMECHANICAL DEVICES; OPTICAL TRANSFER FUNCTION; REFLECTION; SIGNAL TO NOISE RATIO; TISSUE; TOMOGRAPHY;

IMAGING TECHNIQUES;

EID: 12144251717     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.530016     Document Type: Conference Paper

References (19)

1. J. Pawley, ed., Handbook of Biological Confocal Microscopy, 3rd ed., Plenum, New York 1996.
2. L J. Bussau, L.T.Vo, P.M. Delaney, G.D. Papworth, D.H.Barkla, R.G.King, "Fibre optic confocal imaging (FOCI) of keratinocytes, blood vessels and nerves in hairless mouse skin in vivo," Journal of Anatomy, 192, 187-94, (1998).
3. G.D.Papworth, P.MDelaney, L.J.Bussau, L.T.Vo, R.G. King, "In vivo fibre optic confocal imaging of microvasculature and nerves in the rat vas deferens and colon," Journal of Anatomy, 192, 489-95, (1998).
4. Y.S.Sabharwal, A.R.Rouse, L.Donaldso, M.F.Hopkins, A.F.Gmitro, "Slit-Scanning Confocal Microendoscope for High-Resolution In Vivo Imaging," Applied Optics, 38, 7133-44, (1999).
5. F.Helmchen, M.S.Fee, D.W.Tank, W.Denk, "A miniature head-mounted two-photon microscope. high-resolution brain imaging in freely moving animals," Neuron, 31, 903-12, (2001).
6. C.Liang, K.Sung, R.R.Richards-Kortum, M.R.Descour, "Design of a High-Numerical-Aperture Miniature Microscope Objective for an Endoscopic Fiber Confocal Reflectance Microscope," Applied Optics, 41, 4603-10, (2002).
7. D.L.Dickensheets, G.S.Kino,. "Silicon-Micromachined Scanning Confocal Optical Microscope," IEEE Journal of Microelectromechanical Systems, 7, 38-47, (1998).
8. D.L.Dickensheets, G.S.Kino,. "A Micromachined Scanning Confocal Optical Microscope," Optics Letters, 21, 764-766, (1996).
9. E. H. Stelzer, S. Lindek. "Fundamental reduction of the observation volume in far-field light microscopy by detection orthogonal to the illumination axis: confocal theta microscopy," Opt. Comm, 111, 536-547 (1994).
10. E. H. Stelzer, S. Lindek, S. Albrecht, R. Pick, G. Ritter, N. Salmon, R. Stricker. "A new tool for the observation of embryos and other large specimens: confocal theta fluorescence microscopy." Journal of Microscopy, 179, 1-10 (1995).
11. S. Lindek, E. H. K. Stelzer. "Optical transfer functions for confocal theta fluorescence microscopy." J. Opt. Soc. Am. A, 13, 479-82 (1996).
12. R. H. Webb, F. Rogomentich. "Confocal microscope with large field and working distance." Applied Optics, 38, 4870-75 (1999).
13. T.D.Wang , M.J.Mandella , G.S.Kino, "Dual Axes Confocal Microscope for High Resolution In Vivo Imaging." Optics Letters, 28, 414-16, (2003).
14. T.D.Wang, M.J.Mandella, C.H.Contag, N.Y.Chan, G.S.Kino, "Dual Axes Confocal Microscope with Post-Objective Scanning and Low Coherence Heterodyne Detection." Optics Letters, 28, 1915-17, (2003).
15. T.D.Wang, M.J.Mandella, C.H.Contag, N.Y.Chan, G.S.Kino, "Confocal Fluorescence Microscope with Dual Axes Architecture for In Vivo Cellular and Molecular Imaging," Journal Biomedical Optics, in press.
16. T. R. Corle and G. S. Kino. Confocal Scanning Optical Microscopy and Related Imaging Systems, Academic Press, Boston, 1996.
17. M. Born and E. Wolf, Principles of Optics, 7th ed, Cambridge Press, Cambridge, UK, 1999.
18. R.Y.Tsien , "The green fluorescent protein," Annu Rev Biochem., 67, 509-44, (1998).
19. A.K.Hadjantonakis, M.Gertsenstein, M.Ikawa. M. Okabe, A. Nagy. "Generating green fluorescent mice by germline transmission of green fluorescent ES cells." Mech. Dev., 76, 79-90, (1998).