Vitreous humour as a complementary sample to blood for the detection/confirmation of diazepam: Ante-mortem and post-mortem studies in an animal model

Human and Experimental Toxicology

Volumn 23, Issue 12, 2004, Pages 571-577

  Teixeira, Helena M ^a,b   Reis, F ^c   Proenca P ^a   Ramos, P ^c   Quintela, O ^d   Lopez Rivadulla M ^d   Marques, E ^a,b   Vieira, D N ^a,b  

^a INSTITUTO NACIONAL DE MEDICINA LEGAL E CIÊNCIAS FORENSES   (Portugal)

^b UNIVERSITY OF COIMBRA   (Portugal)

^c UNIVERSITY OF COIMBRA   (Portugal)

^d UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

Author keywords

Animal model; Blood; Diazepam; Forensic toxicology; Post mortem; Vitreous humour

Indexed keywords

DIAZEPAM; NORDAZEPAM; PINAZEPAM;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BLOOD SAMPLING; CALCULATION; CONTROLLED STUDY; CORRELATION ANALYSIS; DETERIORATION; DIAGNOSTIC VALUE; DOSE TIME EFFECT RELATION; DRUG ABSORPTION; DRUG DETERMINATION; DRUG ELIMINATION; DRUG HALF LIFE; DRUG OVERDOSE; DRUG TISSUE LEVEL; FORENSIC PATHOLOGY; LIVER METABOLISM; MORTALITY; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE DIAGNOSIS; RABBIT; TISSUE DISTRIBUTION; VITREOUS BODY;

ANIMALS; AQUEOUS HUMOR; DIAZEPAM; POSTMORTEM CHANGES; RABBITS; TISSUE DISTRIBUTION; VITREOUS BODY;

ANIMALIA; ORYCTOLAGUS CUNICULUS;

EID: 11844276042     PISSN: 09603271     EISSN: None     Source Type: Journal    
DOI: 10.1191/0960327104ht490oa     Document Type: Article

References (42)

1. Drummer OH. Toxicology: methods of analysis, post-mortem. In Encyclopedia of forensic science. San Diego, CA: Academic Press, 2000; 1404-409.
2. Kintz P, Tracqui A, Mangin P. Toxicological investigations on unusual material (hair and vitreous humor): interest and limitations. Arch Toxicol Suppl 1992; 15: 282-85.
3. Sturner WQ, Garriott JC. Comparative toxicology in vitreous humor and blood. Forensic Sci 1975; 6: 31-39.
4. +]. J Forensic Sci 2001; 46: 209-14.
5. Fernandez P, Lopez-Rivadulla M, Linares JM, Tato F, Bermejo AM. A comparative pharmacokinetic study of ethanol in the blood, vitreous humor and aqueous humor of rabbits. Forensic Sci Int 1989; 41: 61-65.
6. Mackey-Bojak S, Kloss J, Apple F. Cocaine, cocaine metabolite, and ethanol concentrations in postmortem blood and vitreous humor. J Anal Toxicol 2000; 24: 59-65.
7. Felby S, Olsen J. Comparative studies of postmortem ethyl alcohol in vitreous humor, blood and muscle. J Forensic Sci 1969; 14: 93-101.
8. Caplan YH, Levine B. Vitreous humor in the evaluation of postmortem blood ethanol concentration. J Anal Toxicol 1990; 14: 305-307.
9. Chao TC, Lo DST. Relationship between postmortem blood and vitreous humor ethanol levels. Am J Forensic Med Pathol 1993; 14: 303-308.
10. Bermejo AM, Ramos I, Fernandez P, Lopez-Rivadulla M, Cruz A, Chiarotti M et al. Morphine determination by GC/MS in human vitreous humor and comparison with RIA. J Anal Toxicol 1992; 16: 372-74.
11. Samuel R, Dsiezicz RJ, Longhmanee F. Use of non-normal body fluids in forensic pathology and toxicology. Clin Lab Sci 1994; 1: 100-102.
12. McKinney PE, Phillips S, Gomez HF, Brent J, MacIntyre M, Watson WA. Vitreous humor cocaine and metabolite concentrations: do postmortem specimens reflect blood levels at the time of death? J Forensic Sci 1995; 40: 102-107.
13. Felby S, Olsen J. Comparative studies of postmortem barbiturate and meprobamate in vitreous humor, blood and liver. J Forensic Sci 1969; 14: 507-514.
14. Ziminski KR, Wemyss CT, Bidanset JH, Manning TJ, Lukash L. Comparative study of postmortem barbiturates, methadone, and morphine in vitreous humor, blood and tissue. J Forensic Sci 1984; 29: 903-909.
15. Pragst F, Spiegel K, Leuschner U, Hager A. Detection of 6-acetylmorphine in vitreous humor and cerebrospinal fluid - comparison with urinary analysis for proving heroin administration in opiate fatalities. J Anal Toxicol 1999; 23: 168-72.
16. Rodge S, Hilberg T, Teige B. Fatal combined intoxication with new antidepressants. Human cases and an experimental study of postmortem moclobemide redistribution. Forensic Sci Int 1999; 100: 109-16.
17. Pounder DJ, Jones GR. Post-mortem drug redistribution - A toxicological nightmare. Forensic Sci Int 1990; 45: 253-63.
18. Drummer OH, Gerostamoulos J. Postmortem drug analysis: analytical and toxicological aspects. Ther Drug Monit 2002; 24: 199-209.
19. Levine B, Wu SC, Dixon A, Smialek JE. Site dependence of postmortem blood methadone concentrations. Am J Forensic Med Pathol 1995; 16: 97-100.
20. Levine B, Ramcharitar V, Smialek JE. Stability of ecgonine methyl ester in postmortem urine specimens. J Forensic Sci 1996; 41: 126-28.
21. Moody DE, Monti KM, Spanbauer AC. Long-term stability of abuse drugs and antiabuse chemotherapeutical agents stored at -20°C. J Anal Toxicol 1999; 23: 535-40.
22. Barnhart FE, Fogacci JR, Reed DW. Methamphetamine: a study of postmortem redistribution. J Anal Toxicol 1999; 23: 69-70.
23. Gerostamoulos J, Drummer OH. Postmortem redistribution of morphine and its metabolits. J Forensic Sci 2000; 45: 843-45.
24. Pinho-Marques EG, Teixeira HM, Proença P, Oliveira MM, Castanheira AM, Barroso M et al. Etude rétrospective, depuis les années 50, des toxiques détectés dans les échantillons biologiques humains, de cadavres autopsiés, dans les chefs-lieux appartenant à la circonscription médico-légale de Coimbra. In Vieira DN, Rebelo A, Cordeiro C, Centro de Estudos de Pós-Graduação em Medicina ed. Temas de Medicina Legal II. Coimbra: Coimbra Gráfica, 2002; 407-13.
25. Robertson MD, Drummer OH. Postmortem distribution and redistribution of nitrobenzodiazepines in man. J Forensic Sci 1998; 43: 9-13.
26. Robertson MD, Drummer OH. Postmortem drug metabolism by bacteria. J Forensic Sci 1995; 40: 382-86.
27. El Mahjoub A, Staub C. Stability of benzodiazepines in whole blood samples stored at different temperatures. J Pharm Biomed Anal 2000; 23: 1057-63.
28. Dalpe-Scott M, Degouffe M, Garbutt D, Drost M. A comparison of drug concentrations in post-mortem cardiac and peripheral blood in 320 cases. Can Soc Forensic Sci J 1995; 28: 113-21.
29. Pépin G, Dubourvieux N, Gaillard Y. Difficulté d'interprétation des taux des benzodiazépines et molécules apparentées dans le sang de cadavre prélevé à l'autopsie: étude de leur dégradation in vitro après conservation pendant 6 moins à différentes températures. J Med Legal Droit Medical 1998; 41: 341-53.
30. Levine B, Blanke R, Valentour JC. Postmortem stability of benzodiazepines in blood and tissues. J Forensic Sci 1983; 28: 102-15.
31. Skopp G, Potsch L, Konig I, Mattern R. A preliminary study on the stability of benzodiazepines in blood and plasma stored at 4°C. Int J Legal Med 1998; 111: 1-5.
32. Fernandez P, Hermida I, Bermejo AM, Ramos I, Lopez-Rivadulla M, Cruz A et al. Simultaneous determination of diazepam and its metabolites in plasma by high-performance liquid chromatography. J Liq Chromatogr 1991; 14: 2587-99.
33. Mußhoff F, Daldrup T. A rapid solid-phase extraction and HPLC/DAD procedure for the simultaneous determination and quantification of different benzodiazepines in serum, blood and post-mortem blood. Int J Legal Med 1992; 105: 105-109.
34. Lambert WE, Meyer E, Xue-Ping Y, De Leenheer AP. Screening, identification and quantification of benzodiazepines in postmortem samples by HPLC with photodiode array detection. J Anal Toxicol 1995; 19: 35-40.
35. Logan BK, Stafford DT. Direct analysis of anticonvulsant drugs in vitreous humor by HPLC using a column switching technique. Forensic Sci Int 1989; 41: 125-34.
36. Klotz U, Antonin K-H, Bieck PR. Phamacokinetics and plasma binding of diazepam in man, dog, rabbit, guinea pig and rat. J Pharmacol Exp Ther 1976; 199: 67-73.
37. Pounder DJ. The nightmare of postmortem drug changes. Legal Med 1993; 163-91.
38. Hilberg T, Bugge A, Bevylich KM, Morland J, Bjorneboe A. Diffusion as a mechanism of postmortem redistribution. An experimental study in rats. Int J Legal Med 1992; 105: 87-91.
39. Robertson MD, Drummer OH. Stability of nitrobenzodiazepines in postmortem blood. J Forensic Sci 1998; 43: 5-8.
40. Scott KS, Oliver JS. The use of vitreous humor as an alternative to whole blood for the analysis of benzodiazepines. J Forensic Sci 2001; 46: 694-97.
41. De Letter EA, De Paepe P, Clauwaert KM, Belpaire FM, Lambert WE, Van Bocxlaer JF et al. Is vitreous humor useful for the interpretation of 3,4-methylenedioxy-methamphetamine (MDMA) blood levels? Experimental approach with rabbits. Int J Legal Med 2000; 114: 29-35.
42. Takeshita H, Mogi K, Yasuda T, Mori S, Nakashima Y, Nakajima T et al. Postmortem absorption of dichloromethano: a case study and animal experiments. Int J Legal Med 2000; 111: 96-100.