PCR-based denaturing gradient gel electrophoretictypification and identification of the microbial consortium present in kefir grains

World Journal of Microbiology and Biotechnology

Volumn 20, Issue 7, 2004, Pages 687-693

  Garbers, I M ^a   Britz, T J ^a   Witthuhn, R C ^a  

^a STELLENBOSCH UNIVERSITY   (South Africa)

Author keywords

DGGE; Kefir; Lactic acid bacteria; Lactobacillus; Yeasts

Indexed keywords

BACTERIA; BEVERAGES; COMPOSITION; ELECTROPHORESIS; GELATION; GENES; GENETIC ENGINEERING; MICROBIOLOGY; RNA;

GEL ELECTROPHORETICYPIFICATION; KEFIR GRAINS; LACTOBACILLI BACTERIA; MICROBIOLOGICAL COMPOSITION;

GRAIN (AGRICULTURAL PRODUCT);

BEVERAGES; CHEMICAL COMPOSITION; ELECTROPHORESIS; GELATION; GENES; GRAIN; MICROBIOLOGY;

BACTERIA (MICROORGANISMS); KEFIR; LACTOBACILLUS;

EID: 11844268646     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-004-2624-3     Document Type: Article

References (30)

1. Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D.J. 1997 Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25, 3389-3402.
2. Andrighetto, C., De Dea, P., Lombardi, A., Neviani, E., Rossetti, L. & Giraffa, G. 1998 Molecular identification and cluster analysis of homofermentative thermophylic lactobacilli isolated from dairy products. Research in Microbiology 149, 631-643.
3. Assadi, M.M., Pourahmad, R. & Moazami, N. 2000 Use of isolated kefir starter cultures in kefir production. World Journal of Microbiology and Biotechnology 16, 541-543.
4. Cocolin, L., Bisson, L.F. & Mills, D.A. 2000 Direct profiling of the yeast dynamics in wine fermentation. FEMS Microbiology Letters 189, 81-87.
5. Cocolin, L., Aggio, D., Manzano, M., Cantoni, C. & Comi, G. 2002 An application of PCR-DGGE analysis to profile the yeast populations in raw milk. International Dairy Journal 12, 407-411.
6. Corsetti, A., Lavermicocca, P., Morea, M., Baruzzi, F., Tosti, N. & Gobbetti, M. 2001 Phenotypic and molecular identification and clustering of lactic acid bacteria and yeasts from wheat (species Triticum durum and Triticum aestivum) sourdoughs of Southern Italy. International Journal of Food Microbiology 64, 95-104.
7. Cronjé, M. 2003 Production of Kepi grains using pure cultures as starters. M.Sc. Thesis, Stellenbosch University, Stellenbosch, South Africa.
8. Duitschaever, C.L., Kemp, N. & Emmons, D. 1987 Pure culture formation and procedure for the production of kefir. Milchwissenschaft 42, 80-82.
9. du Plessis, E. & Dicks, L.M.T. 1995 Evaluation of random amplified polymorphic DNA (RAPD)-PCR as a method to differentiate Lactobacillus acidophilus, Lactobacillus crispatus, Lactobacillus amylovorus, Lactobacillus gallinarum, Lactobacillus gasseri and Lactobacillus johnsonii. Current Microbiology 31, 114-118.
10. Felske, A., Rheims, H., Wolterink, A., Stackebrandt, E. & Akkermans, A.D.L. 1997 Ribosome analysis reveals prominent activity of an uncultured member of the class Acinetobacteria in grassland soils. Microbiology 143, 2983-2989.
11. Giraffa, G., De Vecchi, P. & Rossetti, L. 1998 Identification of Lactobacillus delbrueckii subspecies bulgaricus and subspecies lactis dairy isolates by amplified rDNA restriction analysis. Journal of Applied Microbiology 85, 918-924.
12. Gurtner, C., Heyrman, J., Pinar, G., Lubitz, W., Swings, J. & Rölleke, S. 2000 Comparative analysis of the bacterial diversity on two different biodeteriorated wall paintings by DGGE and 16S rDNA sequence analysis. International Biodeterioration and Biodegradation 46, 229-239.
13. Guzel-Seydim, Z., Seydim, A.C. & Greene, A.K. 2000 Organic acids and volatile flavor components evolved during refrigerated storage of kefir. Journal of Dairy Science 83, 275-277.
14. Hillis, D.M. & Bull, J.J. 1993 An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology 42, 182-192.
15. Kawai, M., Matsutera, E., Kanda, H., Yamaguchi, N., Tani, K. & Nasu, M. 2002 16S Ribosomal DNA-based analysis of bacterial diversity in purified water used in pharmaceutical manufacturing processes by PCR and denaturing gradient gel electrophoresis. Applied and Environmental Microbiology 68, 699-704.
16. Kemp, N. 1984 Kefir, the champagne of cultured dairy products. Cultured Dairy Products Journal 19, 29-30.
17. Kuo, C. & Lin, C. 1999 Taiwanese kefir grains: their growth, microbial and chemical composition of fermented milk. Australian Journal of Dairy Science 54, 19-23.
18. Kwak, H.S., Park, S.K. & Kim, D.S. 1996 Biostabilization of kefir with a nonlactose-fermenting yeast. Journal of Dairy Science 79, 937-942.
19. Muyzer, G., de Waal, E.C. & Uitterlinden, A.G. 1993 Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Applied and Environmental Microbiology 59, 695-700.
20. Page, R.D.M. 1996 TREEVIEW: An application to display phylogenetic trees on personal computers. Computerized Applied Bioscience 12, 357-358.
21. Pintado, M.E., Lopes Da Silva, J.A., Fernandes, P.B., Malcata, F.X. & Hogg, T.A. 1996 Microbiological and rheological studies on Portuguese kefir grains. International Journal of Food Science and Technology 31, 15-26.
22. Rambaut, A. 2002 Sequence Alignment Editor v2.0 (programme distributed by the author). Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK.
23. Schoevers, A. & Britz, T.J. 2003 Influence of different culturing conditions on kefir grain increase. International Journal of Dairy Technology 56, 183-187.
24. Swofford, D.L. 2000 PAUP 4.0: Phylogenetic Analysis Using Parsimony. Sinauer Associates, Sunderland, MA, USA.
25. Takizawa, S., Kojima, S., Tamura, S., Fujinaga, S., Benno, Y. & Nakase, T. 1998 The composition of the Lactobacillus flora in kefir grains. Systematic and Applied Microbiology 21, 121-127.
26. Van Elsas, J.D., Mäntynen, V. & Wolters, A.C. 1997 Soil DNA extraction and assessment of the fate of Mycobacterium chlorophenolicum strain PCP-1 in different soils by 16S ribosomal RNA gene sequence based on most probable number PCR and immunofluorescence. Biology and Fertility of Soils 24, 186-188.
27. Walter, J., Tannock, G.W., Tilsala-Timisjarvi, A., Rodtong, S., Loach, D.M., Munro, K. & Alatossava, T. 2000 Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers. Applied and Environmental Microbiology 66, 297-303.
28. Witthuhn, R.C., Schoeman, T. & Britz, T.J. Isolation and characterisation of the microbial population of different South African Kefir grains. International Journal of Dairy Technology 57, 33-37.
29. Wouters, J.T.M., Ayad, E.H.E., Hugenholtz, J. & Smit, G. 2002 Microbes from raw milk for fermented dairy products. International Dairy Journal 12, 91-109.
30. Wyder, M., Keile, L. & Teuber, M. 1999 Description of Saccharomyces turicensis sp. nov., a new species from kephyr. Systematic and Applied Microbiology 22, 420-425.