메뉴 건너뛰기




Volumn 39, Issue 2, 2005, Pages 247-253

Expression and purification of histidine-tagged bacteriophage T7 DNA polymerase

Author keywords

Bacteriophage T7; Metal affinity purification; Polynucleotide polymerase

Indexed keywords

DNA DIRECTED DNA POLYMERASE; HISTIDINE; RECOMBINANT PROTEIN;

EID: 11844256960     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.10.022     Document Type: Article
Times cited : (12)

References (25)
  • 1
    • 0015240411 scopus 로고
    • Deoxyribonucleic acid polymerase of bacteriophage T7
    • P. Grippo, and C.C. Richardson Deoxyribonucleic acid polymerase of bacteriophage T7 J. Biol. Chem. 246 1971 6867 6873
    • (1971) J. Biol. Chem. , vol.246 , pp. 6867-6873
    • Grippo, P.1    Richardson, C.C.2
  • 2
    • 0018801557 scopus 로고
    • T7-induced DNA polymerase. Characterization of associated exonuclease activities and resolution into biologically active subunits
    • S. Adler, and P. Modrich T7-induced DNA polymerase. Characterization of associated exonuclease activities and resolution into biologically active subunits J. Biol. Chem. 254 1979 11605 11614
    • (1979) J. Biol. Chem. , vol.254 , pp. 11605-11614
    • Adler, S.1    Modrich, P.2
  • 3
    • 0023665251 scopus 로고
    • Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7
    • S. Tabor, H.E. Huber, and C.C. Richardson Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7 J. Biol. Chem. 262 1987 16212 16223
    • (1987) J. Biol. Chem. , vol.262 , pp. 16212-16223
    • Tabor, S.1    Huber, H.E.2    Richardson, C.C.3
  • 4
    • 0030889914 scopus 로고    scopus 로고
    • Amino acid changes in a unique sequence of bacteriophage T7 DNA polymerase alter the processivity of nucleotide polymerization
    • X.M. Yang, and C. Richardson Amino acid changes in a unique sequence of bacteriophage T7 DNA polymerase alter the processivity of nucleotide polymerization J. Biol. Chem. 272 1997 6599 6606
    • (1997) J. Biol. Chem. , vol.272 , pp. 6599-6606
    • Yang, X.M.1    Richardson, C.2
  • 5
    • 0028277915 scopus 로고
    • Error-prone replication of repeated DNA sequences by T7 DNA polymerase in the absence of its processivity subunit
    • T.A. Kunkel, S.S. Patel, and K.A. Johnson Error-prone replication of repeated DNA sequences by T7 DNA polymerase in the absence of its processivity subunit Proc. Natl. Acad. Sci. USA 91 1994 6830 6834
    • (1994) Proc. Natl. Acad. Sci. USA , vol.91 , pp. 6830-6834
    • Kunkel, T.A.1    Patel, S.S.2    Johnson, K.A.3
  • 6
    • 0018610971 scopus 로고
    • Deoxyribonucleic acid polymerase of bacteriophage T7. Purification and properties of the phage-encoded subunit, the gene 5 protein
    • K. Hori, D.F. Mark, and C.C. Richardson Deoxyribonucleic acid polymerase of bacteriophage T7. Purification and properties of the phage-encoded subunit, the gene 5 protein J. Biol. Chem. 254 1979 11591 11597
    • (1979) J. Biol. Chem. , vol.254 , pp. 11591-11597
    • Hori, K.1    Mark, D.F.2    Richardson, C.C.3
  • 7
    • 0019463814 scopus 로고
    • Characterization of bacteriophage T7 DNA polymerase purified to homogeneity by antithioredoxin immunoadsorbent chromatography
    • B. Nordström, H. Randahl, I. Slaby, and A. Holmgren Characterization of bacteriophage T7 DNA polymerase purified to homogeneity by antithioredoxin immunoadsorbent chromatography J. Biol. Chem. 256 1981 3112 3117
    • (1981) J. Biol. Chem. , vol.256 , pp. 3112-3117
    • Nordström, B.1    Randahl, H.2    Slaby, I.3    Holmgren, A.4
  • 8
    • 0038333906 scopus 로고
    • Bacteriophage T7 DNA polymerase: Cloning and high-level expression
    • H. Reutiman, B.M. Sjöberg, and A. Holmgren Bacteriophage T7 DNA polymerase: cloning and high-level expression Proc. Natl. Acad. Sci. USA 82 1985 6783 6787
    • (1985) Proc. Natl. Acad. Sci. USA , vol.82 , pp. 6783-6787
    • Reutiman, H.1    Sjöberg, B.M.2    Holmgren, A.3
  • 9
    • 0021919826 scopus 로고
    • A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes
    • S. Tabor, and C.C. Richardson A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes Proc. Natl. Acad. Sci. USA 83 1985 1074 1078
    • (1985) Proc. Natl. Acad. Sci. USA , vol.83 , pp. 1074-1078
    • Tabor, S.1    Richardson, C.C.2
  • 10
    • 0026033193 scopus 로고
    • Pre-steady state kinetic analysis of processive DNA replication including complete characterization of exonuclease-deficient mutants
    • I. Wong, and K.A. Johnson Pre-steady state kinetic analysis of processive DNA replication including complete characterization of exonuclease-deficient mutants Biochemistry 30 1991 511 525
    • (1991) Biochemistry , vol.30 , pp. 511-525
    • Wong, I.1    Johnson, K.A.2
  • 11
    • 0026201479 scopus 로고
    • Rapid isolation of homogeneous cloned T7 gene 5 protein and T7 DNA polymerase by affinity chromatography on immobilized thioredoxin
    • I. Slaby, and A. Holmgren Rapid isolation of homogeneous cloned T7 gene 5 protein and T7 DNA polymerase by affinity chromatography on immobilized thioredoxin Protein Expr. Purif. 2 1991 270 277
    • (1991) Protein Expr. Purif. , vol.2 , pp. 270-277
    • Slaby, I.1    Holmgren, A.2
  • 12
    • 0026327753 scopus 로고
    • A novel strategy for production of a highly expressed recombinant protein in an active form
    • J.R. Blackwell, and R. Horgan A novel strategy for production of a highly expressed recombinant protein in an active form FEBS Lett. 295 1991 10 12
    • (1991) FEBS Lett. , vol.295 , pp. 10-12
    • Blackwell, J.R.1    Horgan, R.2
  • 13
    • 0034082797 scopus 로고    scopus 로고
    • Compatible-solute-supported periplasmatic expression of functional recombinant proteins under stress conditions
    • S. Barth, M. Huhn, B. Matthey, A. Klimka, E.A. Galinski, and A. Engert Compatible-solute-supported periplasmatic expression of functional recombinant proteins under stress conditions Appl. Environ. Microbiol. 66 2000 1572 1579
    • (2000) Appl. Environ. Microbiol. , vol.66 , pp. 1572-1579
    • Barth, S.1    Huhn, M.2    Matthey, B.3    Klimka, A.4    Galinski, E.A.5    Engert, A.6
  • 14
  • 18
    • 0024448151 scopus 로고
    • Calculation of protein extinction coefficient from amino acid sequence data
    • S.C. Gill, and P.H. von Hippel Calculation of protein extinction coefficient from amino acid sequence data Anal. Biochem. 182 1989 319 326
    • (1989) Anal. Biochem. , vol.182 , pp. 319-326
    • Gill, S.C.1    Von Hippel, P.H.2
  • 19
    • 0021099586 scopus 로고
    • T7-induced DNA polymerase. Requirement for thioredoxin sulfhydryl groups
    • S. Adler, and P. Modrich T7-induced DNA polymerase. requirement for thioredoxin sulfhydryl groups J. Biol. Chem. 258 1983 6956 6962
    • (1983) J. Biol. Chem. , vol.258 , pp. 6956-6962
    • Adler, S.1    Modrich, P.2
  • 20
    • 0031441411 scopus 로고    scopus 로고
    • Divergent effects of chaperone overexpression and ethanol supplementation on inclusion body formation in recombinant Escherichia coli
    • J.G. Thomas, and F. Baneyx Divergent effects of chaperone overexpression and ethanol supplementation on inclusion body formation in recombinant Escherichia coli Protein Expr. Purif. 11 1997 289 296
    • (1997) Protein Expr. Purif. , vol.11 , pp. 289-296
    • Thomas, J.G.1    Baneyx, F.2
  • 21
    • 0035955743 scopus 로고    scopus 로고
    • Chemical chaperones regulate molecular chaperones in vitro and in cells under combined salt and heat stresses
    • S. Diamant, N. Eliahu, D. Rosenthal, and P. Goloubinoff Chemical chaperones regulate molecular chaperones in vitro and in cells under combined salt and heat stresses J. Biol. Chem. 276 2001 39586 39591
    • (2001) J. Biol. Chem. , vol.276 , pp. 39586-39591
    • Diamant, S.1    Eliahu, N.2    Rosenthal, D.3    Goloubinoff, P.4
  • 22
    • 0028865891 scopus 로고
    • Production of hydroxyectoine: Cultivation and osmotic downshock of marinococcus strain M52
    • E. Frings, T. Sauer, and E.A. Galinski Production of hydroxyectoine: cultivation and osmotic downshock of marinococcus strain M52 J. Biotechnol. 43 1995 53 61
    • (1995) J. Biotechnol. , vol.43 , pp. 53-61
    • Frings, E.1    Sauer, T.2    Galinski, E.A.3
  • 23
    • 0032518398 scopus 로고    scopus 로고
    • Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 Å resolution
    • S. Doublie, S. Tabor, A.M. Long, C.C. Richardson, and T. Ellenberger Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 Å resolution Nature 391 1998 251 258
    • (1998) Nature , vol.391 , pp. 251-258
    • Doublie, S.1    Tabor, S.2    Long, A.M.3    Richardson, C.C.4    Ellenberger, T.5
  • 24
    • 0035253390 scopus 로고    scopus 로고
    • Fluorescence-based DNA polymerase assay
    • H. Tveit, and T. Kristensen Fluorescence-based DNA polymerase assay Anal. Biochem. 289 2000 96 98
    • (2000) Anal. Biochem. , vol.289 , pp. 96-98
    • Tveit, H.1    Kristensen, T.2
  • 25
    • 0021112553 scopus 로고
    • Two forms of the DNA polymerase of bacteriophage T7
    • M.J. Engler, R.L. Lechner, and C.C. Richardson Two forms of the DNA polymerase of bacteriophage T7 J. Biol. Chem. 258 1983 11165 11173
    • (1983) J. Biol. Chem. , vol.258 , pp. 11165-11173
    • Engler, M.J.1    Lechner, R.L.2    Richardson, C.C.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.