Comparing a ciliate and a fish cell line for their sensitivity to several classes of toxicants by the novel application of multiwell filter plates to Tetrahymena

Research in Microbiology

Volumn 156, Issue 1, 2005, Pages 93-103

  Dayeh, Vivian R ^a   Grominsky, Sherri ^a   DeWitte Orr, Stephanie J ^a   Sotornik, Dana ^a   Yeung, Christine R ^a   Lee, Lucy E J ^b   Lynn, Denis H ^c   Bols, Niels C ^a  

^a UNIVERSITY OF WATERLOO   (Canada)

^b WILFRID LAURIER UNIVERSITY   (Canada)

^c UNIVERSITY OF GUELPH   (Canada)

Author keywords

Cell viability assay; Ciliate; Copper; Gliotoxin; Multiwell filter plate; Resazurin; RTgill W1; Tetrahymena thermophila; Triton X 100

Indexed keywords

ACETOACETIC ACID DERIVATIVE; CARBOXYFLUORESCEIN; COPPER; ESTER DERIVATIVE; FLUORESCENT DYE; GLIOTOXIN; TRITON X 100;

ANIMAL CELL; ARTICLE; CELL ASSAY; CELL VIABILITY; CENTRIFUGATION; CILIATE; CONTROLLED STUDY; DOSE RESPONSE; ENERGY METABOLISM; NONHUMAN; PRESSURE; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; TETRAHYMENA THERMOPHILA; TROUT; VACUUM;

ANIMALS; CELL LINE; CELL MEMBRANE PERMEABILITY; CELL SURVIVAL; COPPER SULFATE; ENERGY METABOLISM; FLUORESCEINS; GLIOTOXIN; OCTOXYNOL; ONCORHYNCHUS MYKISS; OXAZINES; TETRAHYMENA THERMOPHILA; TOXICITY TESTS; XANTHENES;

CILIOPHORA; MYXOZOA; ONCORHYNCHUS MYKISS; PROTOZOA; SALMONIDAE; TETRAHYMENA; TETRAHYMENA THERMOPHILA;

EID: 11344295650     PISSN: 09232508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.resmic.2004.08.005     Document Type: Article

References (31)

1. L. Benitez, A. Martin-Gonzales, P. Gilardi, T. Soto, J.R. Declecea, and J.C. Gutierrez The ciliated protozoa Tetrahymena thermophila as a biosensor to detect mycotoxins Letts. Appl. Microbiol. 19 1994 489 491
2. J.P. Bijl, D.M. Rousseau, D.G. Dive, and C.H. van Peteghem Potentials of a synchronized culture of Tetrahymena pyriformis for toxicity studies of mycotoxins J. Assoc. Off. Anal. Chem. 71 1988 282 285
3. P. Bogaerts, J. Senaud, and J. Bohatier Bioassay technique using nonspecific esterase activities of Tetrahymena pyriformis for screening and assessing cytotoxicity of xenobiotics Environ. Tox. Chem. 17 1998 1600 1605
4. N.C. Bols, A. Barlian, M. Chirino-Trejo, S.J. Caldwell, P. Goegan, and L.E.J. Lee Development of a cell line from primary cultures of rainbow trout, Oncorhynchus mykiss (Walbaum), gills J. Fish Dis. 17 1994 601 611
5. J.L. Bonnet, M. Dusser, J. Bohatier, and J. Laffosse Cytotoxicity assessment of three therapeutic agents, cyclosporin-A, cisplatin and doxorubicin, with the ciliated protozoan Tetrahymena pyriformis Res. Microbiol. 154 2003 375 385
6. J.W. Carter, and I.L. Cameron Toxicity bioassay of heavy metals in water using Tetrahymena pyriformis Water Res. 7 1973 951 961
7. V.R. Dayeh, K. Schirmer, and N.C. Bols Applying whole-water samples directly to fish cell cultures in order to evaluate the toxicity of industrial effluent Water Res. 36 2002 3727 3738
8. V.R. Dayeh, K. Schirmer, L.E.J. Lee, and N.C. Bols The use of fish-derived cell lines for investigation of environmental contaminants Current Protocols in Toxicology 2003 Wiley New York 1.5.1 1.5.17
9. V.R. Dayeh, S.L. Chow, K. Schirmer, D.H. Lynn, and N.C. Bols Evaluating the toxicity of Triton X-100 to protozoan, fish and mammalian cells using fluorescent dyes as indicators of cell viability Ecotoxicol. Environ. Saf. 57 2004 375 382
10. N. Dias, A. Nicolau, G.S. Carvalho, M. Mota, and N. Lima Miniaturization and application of the MTT assay to evaluate metabolic activity of protozoa in the presence of toxicants J. Basic Microbiol. 39 1999 103 108
11. N. Dias, and N. Lima A comparative study using a fluorescence-based and a direct-count assay to determine cytotoxicity in Tetrahymena pyriformis Res. Microbiol. 153 2002 313 322
12. N. Dias, R.A. Mortara, and N. Lima Morphological and physiological changes in Tetrahymena pyriformis for the in vitro cytotoxicity assessment of Triton X-100 Toxicol. in Vitro 17 2003 357 366
13. R.C. Ganassin, K. Schirmer, and N.C. Bols Methods for the use of fish cell and tissue cultures as model systems in basic and toxicology research G.K. Ostrander The Laboratory Fish 1999 Academic Press San Diego 631 651 chap. 38
14. G. Gilron, S.G. Gransden, D.H. Lynn, J. Broadfoot, and R. Scroggins A behavioral toxicity test using the ciliated protozoan Tetrahymena thermophila: I. Method description Environ. Tox. Chem. 18 1999 1813 1816
15. A.M. Gonzalez, L. Benitez, T. Soto, J.R. De Lecea, and J.C. Gutierrez A rapid bioassay to detect mycotoxins using a melanin precursor overproducer mutant of the ciliate Tetrahymena thermophila Cell Biol. Int. 21 1997 213 216
16. M. Gulden, S. Morchel, and H. Seibert Factors influencing nominal effective concentrations of chemical compounds in vitro: Cell concentration Water Res. 7 2001 233 243
17. H.C. Huber, W. Huber, and U. Ritter Simple bioassays for evaluating toxicity of environmental chemicals using microcultures of human peripheral blood lymphocytes and monoxenic cultures of the ciliate Tetrahymena pyriformis ZB Hyg. Umweltmed. 189 1990 511 526
18. D.H. Lynn, and J.O. Corliss Ciliophora F.W. Harrison J.O. Corliss Microscopic Anatomy of Invertebrates, Protozoa, vol. 1 1991 Wiley-A. R. Liss New York 333 467
19. A. Nicolau, M. Mota, and N. Lima Physiological responses of Tetrahymena pyriformis to copper, zinc, cycloheximide and Triton X-100 FEMS Microbiol. Ecol. 30 1999 209 216
20. A. Nicolau, N. Dias, M. Mota, and N. Lima Trends in the use of protozoa in the assessment of wastewater treatment Res. Microbiol. 152 2001 621 630
21. J.R. Nilsson Tetrahymena in cytotoxicology: With special reference to effects of heavy metals and selected drugs Eur. J. Protist. 25 1989 2 25
22. F. Nistiar, J. Hrusovsky, J. Mojzis, P. Mizik, and A. Nistiarova A biological method of the determination of mycotoxins - Tetrahymena pyriformis Vet. Med. 31 1986 245 250
23. W.J.V. Osterhout On the importance of physiologically balanced solutions for plants: I. Marine plants Bot. Gaz. 42 1906 127 134
24. M.P. Sauvant, D. Pepin, J. Bohatier, C.A. Goliere, and A. Veyre Comparative study of two in vitro models (L-929 fibroblasts and Tetrahymena pyriformis GL) for the cytological evaluation of packaged water Sci. Total Environ. 156 1994 159 167
25. M.P. Sauvant, D. Pepin, J. Bohatier, C.A. Groliere, and J. Guillo Toxicity assessment of 16 inorganic environmental pollutants by six bioassays Ecotox. Environ. Saf. 37 1997 131 140
26. M.P. Sauvant, D. Pepin, and E. Piccinni Tetrahymena pyriformis: A tool for toxicological studies - A review Chemosphere. 38 1999 1631 1669
27. D. Schlenk, and C.T. Moore Effect of pH and time on the acute toxicity of copper sulfate to the ciliate protozoan Tetrahymena thermophila Bull. Environ. Contam. Toxicol. 53 1994 800 804
28. T.W. Schultz Tetratox: Tetrahymena pyriformis population growth impairment endpoint - A surrogate for fish lethality Toxicol. Method. 7 1997 289 309
29. K. Schirmer, A.G.J. Chan, B.M. Greenberg, D.G. Dixon, and N.C. Bols Methodology for demonstrating and measuring the photocytotoxicity of fluoranthene to fish cells in culture Toxicol. in Vitro. 11 1997 107 119
30. Society of Protozoologists A catalogue of laboratory strains of free-living and parasitic protozoa J. Protozool. 5 1958 1 38
31. P. Waring, and J. Beaver Gliotoxin and related epipolythiodioxopiperazines Gen. Pharmacol. 27 1996 1311 1316