Intracellular cytokines in blood T cells in lung transplant patients - A more relevant indicator of immunosuppression than drug levels

Clinical and Experimental Immunology

Volumn 139, Issue 1, 2005, Pages 159-164

  Hodge, Greg ^a,b   Hodge, S ^a   Reynolds, P ^a   Holmes, M ^a  

^a ROYAL ADELAIDE HOSPITAL   (Australia)

^b WOMEN S AND CHILDREN S HOSPITAL   (Australia)

Author keywords

Flow cytometry; Immunosuppression; Intracellular cytokines; Lung transplant; T cells

Indexed keywords

AZATHIOPRINE; CD4 ANTIGEN; CD8 ANTIGEN; CYCLOSPORIN A; CYTOKINE; IMMUNOSUPPRESSIVE AGENT; INTERLEUKIN 2; PREDNISOLONE; TACROLIMUS; TUMOR NECROSIS FACTOR ALPHA;

ADULT; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; CYTOKINE PRODUCTION; FLOW CYTOMETRY; GENE EXPRESSION; GRAFT REJECTION; HUMAN; HUMAN CELL; IMMUNE DEFICIENCY; IMMUNOMODULATION; IMMUNOSUPPRESSIVE TREATMENT; IN VITRO STUDY; LUNG TRANSPLANTATION; MORBIDITY; MORTALITY; PRIORITY JOURNAL; T LYMPHOCYTE; T LYMPHOCYTE SUBPOPULATION; TECHNIQUE;

ADULT; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; CYTOKINES; FLOW CYTOMETRY; HUMANS; IMMUNOSUPPRESSION; INTERFERON TYPE II; INTERLEUKIN-2; LUNG TRANSPLANTATION; LYMPHOCYTE COUNT; T-LYMPHOCYTES; TRANSFORMING GROWTH FACTOR BETA; TUMOR NECROSIS FACTOR-ALPHA;

EID: 11244351772     PISSN: 00099104     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2249.2005.02671.x     Document Type: Article

References (27)

1. Sundaresan S, Alevy YG, Steward N et al. Cytokine gene transcripts for tumor necrosis factor-alpha, interleukin-2, and interferon-gamma in human pulmonary allografts. J Heart Lung Transplant 1995; 14:512-8.
2. Neuringer IP, Walsh SP, Mannon RB, Gabriel S, Aris RM. Enhanced T cell cytokine gene expression in mouse airway obliterative bronchiolitis. Transplantation 2000; 69:399-405.
3. El-Gamel A, Sim E, Hasleton P et al. Transforming growth factor beta (TGF-beta) and obliterative bronchiolitis following pulmonary transplantation. J Heart Lung Transplant 1999; 18:828-37.
4. Scott LJ, McKeage K, Keam SJ, Plosker G. Tacrolimus: a further update of its use in the management of organ tranplantation. Drugs 2003; 63:1247-97.
5. Lehman C, Wilkening A, Lieber N, Markus R, Krug N, Pabst R, Tschernig T. Lymphocytes in the bronchiolar space reenter the lung tissue by means of the alveolar epithelium, migrate to regional lymph nodes, and subsequently rejoin the systemic immune system. Anat Rec 2001; 264:229-36.
6. Jordan SC, Marchevski A, Ross D, Toyoda M, Waters PF. Serum interleukin-2 levels in lung transplant recipients: correlation with findings on transbronchial biopsy. J Heart Lung Transplant 1992; 11:1001-4.
7. Rondeau E, Cerrina J, Delarue F et al. Tumor necrosis alpha (TNF-alpha) production by cells of bronchiolar lavage (BAL) and peripheral blood mononuclear cells (PBMC) in cardiopulmonary transplant patients. Transplant Proc 1990; 22:1855-6.
8. Hodge G, Hodge S, Han P. Increased levels of apoptosis of leucocyte subsets in PBMCs compared to whole blood as shown by Annexin V binding-relevance to cytokine production. Cytokine 2000; 12:1763-8.
9. Hodge G, Flower R, Han P. Australian plasma-derived factor VIII concentrate inhibits Th1 type cytokines but increases Th2 type cytokine, IL-10. Br J Haematol 2000; 109:902-3.
10. Hodge G, Han P. Factor VIII concentrate inhibits T-helper-type 2 (Th2) cytokine production in vitro: relevance to inhibitor antibody formation. Haemophilia 2001; 7:490-6.
11. Ahmed M, Venkataraman R, Logr A et al. Quantitation of immunosuppression by Tacrolimus using flow cytometricanalysis of IL-2 and IFNγ inhibition in CD8- and CD8+ peripheral blood T cells. Therap Drug Monit 2001; 23:354-62.
12. Stadler M, Birsan T, Holme B, Haririfar M, Scandling J, Morris R. Therap Drug Monit 2003; 22:22-7.
13. Romagnani S. The Th1/Th2 paradigm. Immunol Today 1997; 18:263-6.
14. Kehrl JH. Transforming growth factor-β: An important mediator of immunoregulation. Int J Cell Clon 1991; 9:438-50.
15. Chan SY, Goodman RE, Szmuszkovicz JR, Roessler B, Eichwald EJ, Bishop DK. DNA-liposome versus adenoviral mediated gene transfer of TGF beta1 in vascularised cardiac allografts: differential sensitivity of CD4+ and CD8+ T cells to TGF beta1. Transplantation 2000; 70:1292-301.
16. Hodge S, Hodge G, Flower R, Han P. Methyl prednisolone upregulates monocyte IL-10 production in stimulated whole blood. Scand J Immunol 1999; 49:548-53.
17. Hodge G, Hodge S, Han P. Allium sativum (Garlic) suppresses leucocyte inflammatory cytokine production in vitro: potential therapeutic use in the treatment of inflammatory bowel disease. Cytometry 2002; 48:209-15.
18. Andersson J, Nagy S, Groth CG, Andersson U. Effects of FK506 and cyclospotin A on cytokine production studied in vitro at a single-cell level. Immunol 1992; 75:136-42.
19. Snijdewint FG, Kapsenberg ML, Warben-Penris PJ, Bos ID. Corticosteroids class-dependently inhibited in vitro Th1 and Th2-type cytokine production. Immunopharm 1995; 29:93-101.
20. Berthou C, Legros-Maida S, Soulie A, Wargnier A, Guillet J, Lafaurie C, Stern M, Sasportes M, Israel-Biet D. Expansion of peripheral blood perforin+ CD8+ T-cell subset in long term survival lung transplant patients. Transplant Proc 1996; 28:1964-7.
21. + T-cells by transforming-β. J Immunol 1993; 151:668-77.
22. Widmer MB, Grabstein KH. Regulation of cytolytic T-lymphocyte generation by B-cell stimulatory factor. Nature 1987; 326:795-8.
23. Hu H, Dong Y, Feng P, Fechner J, Hamawy M, Knechtle SJ. Effect of immunosuppressants on T-cell subsets observed in vivo using carboxy-fluorescein diacetate succinimidyl ester labelling. Transplant 2003; 75:1075-7.
24. Sempowski M, Sakiyama S, Tanida N, Fukumoto T, Monden Y, Uyama T. Interleukin-4 and interferon-gamma discordantly regulate collagen biosynthesis by functionally distinct lung fibroblast subsets. J Cell Physiol 1996; 167:290-6.
25. El-Gamel A, Awad M, Sim E, Hasleton P, Yonan N, Egan J et al. Transforming growth factor beta (TGF-beta) and obliterative bronchiolitis following pulmonary transplantation. Eur J Cardiothorac Surg 1998; 13:424-30.
26. Kuypers DR, Claes K, Evenpoel P, Maes B, Vanrenterghem Y. Clinical efficacy and toxicity profile of tacrolimus and mycophenolic acid in relation to combined long-term phermacokinetics in de novo renal allograft recipients. Clin Pharmacol Ther 2004; 75:434-47.
27. Shapiro R. Low toxicity immunosuppressive protocols in renal transplantation. Keio J Med 2004; 53:18-22.