Characterization of tylM3/tylM2 and mydC/mycB pairs required for efficient glycosyltransfer in macrolide antibiotic biosynthesis

Journal of the American Chemical Society

Volumn 126, Issue 51, 2004, Pages 16726-16727

  Melançon III, Charles E ^a   Takahashi, Haruko ^a   Liu, Hung Wen ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MACROLIDE;

AMINO ACID SUBSTITUTION; ARTICLE; BIOSYNTHESIS; BIOTECHNOLOGY; CONTROLLED STUDY; DRUG ACTIVATION; DRUG SYNTHESIS; FUNGAL GENE; GENE CONSTRUCT; GENE EXPRESSION; GENE MUTATION; GENETIC CODE; INTERMETHOD COMPARISON; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; SACCHAROMYCES; SACCHAROPOLYSPORA VENEZUELAE; SEQUENCE HOMOLOGY; STRUCTURE ANALYSIS; WILD TYPE;

ANTI-BACTERIAL AGENTS; GLYCOSYLTRANSFERASES; MACROLIDES; MICROMONOSPORA; SACCHAROPOLYSPORA; STREPTOMYCES;

EID: 11244310524     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja043900e     Document Type: Article

References (17)

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5. Not all glycosyltransferase genes in macrolide and anthracycline antibiotic clusters have desVIII homologues. Examples of such glycosyltransferases that probably act alone are EryBV from the erythromycin pathway, OleG2 from the oleandomyctn pathway, and TylN from the tylosin pathway.
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13. See Supporting Information for experimental details.
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15. While growth under conditions favoring the production of narbonolide (4) was not attempted, it is expected that this 14-membered ring aglycone is also a substrate for TylM2/TylM3 and MycB/MydC pairs.
16. The mydC gene was originally proposed to encode a TDP-sugar 3,4-isomerase (ref 6).
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