Standardization and comparison of endogenous erythroid colony assays performed with bone marrow or blood progenitors for the diagnosis of polycythemia vera

Hematology Journal

Volumn 5, Issue 2, 2004, Pages 161-167

  Dobo, Irène ^a   Donnard, Magali ^b   Girodon, François ^c   Mossuz, Pascal ^d   Boiret, Nathalie ^e   Boukhari, Rachida ^c   Allégraud, Annie ^b   Bascans, Elisabeth ^f   Campos, Lydia ^g   Pineau, Danielle ^h   Turlure, Pascal ^b   Praloran, Vincent ^f   Hermouet, Sylvie ^h  

^a Centre Hospitalier Universitaire   (France)

^b LIMOGES UNIVERSITY HOSPITAL   (France)

^c DIJON UNIVERSITY HOSPITAL   (France)

^d GRENOBLE UNIVERSITY HOSPITAL   (France)

^e Laboratoire d'Hematologie   (France)

^f BORDEAUX UNIVERSITY HOSPITAL   (France)

^g UNIVERSITY HOSPITAL OF SAINT ETIENNE   (France)

^h NANTES UNIVERSITY HOSPITAL   (France)

Author keywords

Collagen; Endogeneous erythroid colony formation; Idiopathic erythrocytosis; Polycythemia vera; Serum free cultures; Standardization

Indexed keywords

COLLAGEN; CYTOKINE;

ARTICLE; BURST FORMING UNIT E; CELL GROWTH; CLONOGENIC ASSAY; CONTROLLED STUDY; CULTURE MEDIUM; ERYTHROCYTOSIS; ERYTHROID PRECURSOR CELL; ERYTHROPOIESIS; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; LIVING DONOR; MAJOR CLINICAL STUDY; PERIPHERAL BLOOD MONONUCLEAR CELL; PERIPHERAL BLOOD STEM CELL; POLYCYTHEMIA VERA; RELIABILITY; STANDARDIZATION; STATISTICAL SIGNIFICANCE;

BONE MARROW; CELL CULTURE TECHNIQUES; CELLS, CULTURED; COLONY-FORMING UNITS ASSAY; CULTURE MEDIA, SERUM-FREE; ERYTHROID PROGENITOR CELLS; FEMALE; HUMANS; MALE; POLYCYTHEMIA; POLYCYTHEMIA VERA;

EID: 11144354826     PISSN: 14664860     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.thj.6200344     Document Type: Article

References (16)

1. Berlin NI. Diagnosis and classification of the polycythemias. Semin Hematol 1975; 12: 339-351.
2. Pearson TC, Messinezy M. The diagnostic criteria of polycythaemia rubra vera. Leuk Lymphoma 1996; 22 (Suppl 1): 87-93.
3. Westwood NB, Pearson TC. Diagnostic applications of haematopoietic progenitor culture techniques in polycythemias and thrombocythaemias. Leuk Lymphoma 1996; 22 (Suppl 1): 95-103.
4. Pearson TC. Diagnosis and classification of erythrocytoses and thrombocytoses. Baillieres Clin Haematol 1998; 11: 695-720.
5. Michiels JJ, Barbui T, Finazzi G, Fuchtman SM, Kutti J, Rain JD et al. Diagnosis and treatment of polycythemia vera and possible future study designs of PVSG. Leuk Lymphoma 2000; 36: 239-253.
6. Zanjani ED, Lutton JD, Hoffman R, Wasserman LR. Erythroid colony formation by polycythemia vera bone marrow in vitro. Dependence on erythropoietin. J Clin Invest 1977; 59: 841-848.
7. Cashman J, Henkelman D, Humphries K, Eaves C, Eaves A. Individual BFU-E in polycythemia vera produce both erythropoietin dependent and independent progeny. Blood 1983; 61: 876-884.
8. Dobo I, Pincau D, Zandecki M, Hunault M, Hermouet S. Endogenous erythroid and megakaryocytic colony formation in serum-free, cytokine-free collagen gels. J Hematother Stem Cell Res 1999; 8: 601-607.
9. Dobo I, Mossuz P, Campos L, Girodon F, Allégraud A, Latger-Cannard V et al. Comparison of four serum-free, cytokine-free media for analysis of endogenous erythroid colony growth in polycythemia vera and essential thrombocythemia. Hematol J 2001; 2: 396-403.
10. Lanotte M. Terminal differentiation of hemopoietic cell clones cultured in tridimentional collagen matrix: in situ cell morphology and enzyme histochemistry study. Biol Cell 1984; 50: 107-120.
11. Lutton JD, Levere RD. Endogenous erythroid colony formation by peripheral blood mononuclear cells from patients with myelofibrosis and polycythemia vera. Acta Haematol 1979; 62: 94-99.
12. Biljanovic-Paunovic L, Ruvidic R, Pavlovic-Kentera V. Endogenous BFU-E in peripheral blood in diagnosis of polycythemia vera. Eur J Hematol 1990; 45: 262-266.
13. Masters GS, Baines P, Jacobs A. Erythroid colony growth from peripheral blood and bone marrow in polycythemia. J Clin Pathol 1990; 43: 937-941.
14. Shih LY, Lee CT, See LC, On YC, Dunn P, Wang PN et al. In vitro culture growth of erythroid progenitors and serum erythropoietin assay in the differential diagnosis of polycythemia. Eur J Clin Invest 1998; 28: 569-576.
15. Dobo I, Allegraud A, Navenot JM, Boasson M, Bidet J-M, Praloran V. Collagen matrix: an attractive alternative to agar and methylcellulose for the culture of haematopoietic progenitors in autologous transplantation products. J Hematother 1995; 4: 281-287.
16. Dobo I, Bidet J-M, Acquart S, Allegraud A, Amiot L, Boccaccio C et al. Reproducible scoring of CFU-GM and BFU-E grown in collagen-based semi-solid medium after a short (3 h) training. J Hematother 1999; 8: 45-51.