Human recombinant thiamine triphosphatase: Purification, secondary structure and catalytic properties

International Journal of Biochemistry and Cell Biology

Volumn 36, Issue 7, 2004, Pages 1348-1364

  Lakaye, Bernard ^a   Makarchikov, Alexander F ^a,b   Wins, Pierre ^a   Margineanu, Ilca ^a   Roland, Séverine ^a   Lins, Laurence ^a   Aichour, Ridha ^a   Lebeau, Luc ^c   El Moualij, Benaïssa ^a   Zorzi, Willy ^a   Coumans, Bernard ^a   Grisar, Thierry ^a   Bettendorff, Lucien ^a  

^a UNIVERSITY OF LIÈGE   (Belgium)

^b INSTITUTE OF BIOCHEMISTRY   (Belarus)

^c UNIVERSITÉ DE STRASBOURG   (France)

Author keywords

4,4 diisothiocyanatostilbene 2,2 disulfonate; Bovine serum albumin; BSA; DEPC; DIDS; Diethylpyrocarbonate; Fourier transform infrared spectroscopy; FTIR; Glutathione S transferase; GST; hThTPase; Human recombinant thiamine triphosphatase; IPTG

Indexed keywords

CALCIUM ION; CARBOXYL GROUP; DIETHYL PYROCARBONATE; MAGNESIUM; RECOMBINANT ENZYME; THIAMINE TRIPHOSPHATASE; ZINC;

ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; ESCHERICHIA COLI; HUMAN; PH MEASUREMENT; PROTEIN EXPRESSION;

ADENOSINE TRIPHOSPHATE; BINDING SITES; CATALYSIS; CATIONS, DIVALENT; CEREBELLUM; CLONING, MOLECULAR; DIETHYL PYROCARBONATE; DNA, COMPLEMENTARY; ENZYME ACTIVATION; ENZYME STABILITY; ESCHERICHIA COLI; HUMANS; MOLECULAR STRUCTURE; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; THIAMIN-TRIPHOSPHATASE; THIAMINE TRIPHOSPHATE;

BOVINAE; MAMMALIA;

EID: 11144354607     PISSN: 13572725     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biocel.2003.11.013     Document Type: Article

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