Whole cells of Bacillus subtilis AF 1 proved more effective than cell-free and chitinase-based formulations in biological control of citrus fruit rot and groundnut rust

Canadian Journal of Microbiology

Volumn 50, Issue 9, 2004, Pages 737-744

  Manjula, K ^a,b   Kishore, G Krishna ^a   Podile, A R ^a  

^a UNIVERSITY OF HYDERABAD   (India)

^b CENTRE FOR CELLULAR AND MOLECULAR BIOLOGY   (India)

Author keywords

Biocontrol; Chitinase; Lemon fruit rot; Peanut rust

Indexed keywords

AFFINITY CHROMATOGRAPHY; CELLS; CHITIN; ENZYME INHIBITION; ENZYMES; FRUITS; HYDROLYSIS; PATHOLOGY; PROTEINS;

BIOCONTROL AGENTS; HYDROLYTIC ENZYMES; PATHOGENIC FUNGI; POSTHARVEST BIOCONTROL SYSTEMS;

BACTERIA;

ALGINIC ACID; CHITINASE;

ANTAGONISM; BACTERIUM; BIOCONTROL AGENT; BIOLOGICAL CONTROL; FUNGAL DISEASE;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; ASPERGILLUS NIGER; BACILLUS SUBTILIS; BACILLUS SUBTILIS AF1; BACTERIAL CELL WALL; BIOASSAY; BIOLOGICAL PEST CONTROL; CELL FREE SYSTEM; CITRUS FRUIT; CONTROLLED STUDY; CYTOLYSIS; ENZYME MECHANISM; LEMON; MICROORGANISM DETECTION; MOLECULAR WEIGHT; NONHUMAN; PATHOGENESIS; PEANUT; PLANT DAMAGE; PLANT LEAF; PLANT PATHOGEN INTERACTION; POSTHARVEST PERIOD; PRIORITY JOURNAL; PUCCINIA ARACHIDIS; STATISTICAL SIGNIFICANCE;

ACETYLGLUCOSAMINIDASE; ARACHIS HYPOGAEA; ASPERGILLUS NIGER; BACILLUS SUBTILIS; BASIDIOMYCOTA; CHITINASE; CITRUS; PEST CONTROL, BIOLOGICAL; PLANT DISEASES;

ANIMALIA; ARACHIS HYPOGAEA; ASPERGILLUS; ASPERGILLUS NIGER; BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); CITRUS; CITRUS LIMON; FUNGI; NEGIBACTERIA; PUCCINIA;

EID: 11144340288     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/w04-058     Document Type: Article

References (28)

1. Andrews, J.H. 1992. Biological control in the phyllosphere. Annu. Rev. Phytopathol. 12: 603-635.
2. Baker, C.J., Staveley, J.R., and Mock, N. 1985. Biocontrol of bean rust by Bacillus subtilis under field conditions. Plant Dis. 69: 770-772.
3. di Pietro, A., Lorito, M., Hayes, C.K., Broadway, R.M., and Harman, G.E. 1993. Endochitinase from Gliocladium virens: isolation, characterization and synergistic antifungal activity in combination with gliotoxin. Phytopathology, 83: 308-313.
4. Downing, K.J., and Thomson, J.A. 2000. Introduction of the Serratia marcescens chiA gene into an endophytic Pseudomonas fluorescens for the biocontrol of phytopathogenic fungi. Can. J. Microbiol. 46: 363-369.
5. Drapeau, G. 1974. Protease from Staphylococcus aureus. In Methods in enzymology 45B. Edited by L. Lorand. Academic Press, New York.
6. El Ghaouth, A., Wilson, C.L., Wisniewski, M., Droby, S., Smilanick, J.L., and Korsten, L. 2002. Biological control of postharvest diseases of citrus fruits. In Biological control of crop diseases. Edited by S.S. Gnanamanickam. Marcel Dekker, Inc., New York. pp. 289-312.
7. El-Katatny, M.H., Gudelj, M., Robra, K.H., Elnaghy, M.A., and Gubitz, G.M. 2001. Characterization of a chitinase and an endo-β-1,3-glucanase from Trichoderma harzianum Rifai T24 involved in control of the phytopathogen Sclerotium rolfsii. Appl. Microbiol. Biotechnol. 56: 137-143.
8. Frankowski, J., Lorito, M., Scala, F., Schmid, R., Berg, G., and Bahl, H. 2001. Purification and properties of two chitinolytic enzymes of Serratia plymuthica HRO-C48. Arch. Microbiol. 176: 421-426.
9. Gomes, R.C., Semedo, L.T.A.S., Soares, R.M.A., Linhares, L.F., Ulhoa, C.J., Alviano, C.S., and Coelho, R.R.R. 2001. Purification of a thermostable endochitinase from Streptomyces RC1071 isolated from a cerrado soil and its antagonism against phyto-pathogenic fungi. J. Appl. Microbiol. 90: 653-661.
10. Gunaratna, K.R., and Balasubramanian, R. 1994. Partial purification and properties of extracellular chitinase produced by Acremonium obclavatum, an antagonist to the groundnut rust, Puccinia arachidis. World J. Microbiol. Biotechnol. 10: 342-345.
11. Kokalis-Burelle, N., Backinan, P.A., Rodriguez-Kabana, R., and Ploper, L.D. 1992. Potential for biological control of early leafspot of peanut using Bacillus cereus and chitin as foliar amendments. Biol. Control, 2: 321-328.
12. Kumar, B.S.D., Podile, A.R., and Dube, H.C. 1988. Antagonistic acitivity of Bacillus subtilis towards Rhizopus nigricans. J. Biocontrol, 2: 4-8.
13. 4. Nature (London), 277: 680-685.
14. Limon, M.C., Pintor-Toro, J.A., and Benitez, T. 1999. Increased antifungal activity of Trichoderma harzianum transformants that overexpress a 33-kDa chitinase. Phytopathology, 89: 254-261.
15. Manjula, K., and Podile, A.R. 2001. Chitin-supplemented formulations improve biocontrol and plant growth promoting efficiency of Bacillus subtilis AF 1. Can. J. Microbiol. 47: 618-625.
16. Mathivanan, N., Kabilan, V., and Murugesan, K. 1998. Purification, characterization, and antifungal activity of chitinase from Fusarium chlamydosporum, a mycoparasite to groundnut rust, Puccinia arachidis. Can. J. Microbiol. 44: 646-651.
17. Meena, B., Radhajeyalakshmi, R., Marimuthu, T., Vidhyasekaran, P., and Velazhahan, R. 2002. Biological control of groundnut late leaf spot and rust by seed and foliar applications of a powder formulation of Pseudomonas fluorescens. Biocontrol Sci. Technol. 12: 195-204.
18. Ordentlich, A., Elad, Y., and Chet, I. 1988. The role of chitinase of Serratia marcescens in biocontrol of Sclerotium rolfsii. Phytopathology, 78: 84-88.
19. Pan, S.Q., Ye, X.S., and Kuc, J. 1991. Association of β-1,3-glucanase activity and isoform pattern with systemic resistance to blue mould in tobacco induced by stem injection with Peronospora tabacina or leaf inoculation with tobacco mosaic virus. Physiol. Mol. Plant Pathol. 39: 25-39.
20. Podile, A.R., and Kishore, O.K. 2002. Biological control of peanut diseases. In Biological control of crop diseases. Edited by S.S. Gnanamanickam. Marcel Dekker, Inc., New York. pp. 131-160.
21. Podile, A.R., and Prakash, A.P. 1996. Lysis and biological control of Aspergillus niger by Bacillus subtilis AF 1. Can. J. Microbiol. 42: 533-538.
22. Podile, A.R., Prasad, G.S., and Dube, H.C. 1985. Bacillus subtilis AF 1 is an antagonist of vascular wilt pathogens. Curr. Sci. 54: 864-865.
23. Reissig, J.L., Strominger, J.L., and Leloir, L.F. 1955. A modified colorimetric method for the estimation of N-acetylamino sugars. J. Biol. Chem. 217: 959-966.
24. Sakai, K., Yokota, A., Kurokawa, H., Wakayama, M., and Moriguchi, M. 1998. Purification and characterization of three thermostable endochitinases of a noble Bacillus strain, MH-1, isolated from chitin-containing compost. Appl. Environ. Microbiol. 64: 3397-3402.
25. Shapira, R., Ordentlich, A., Chet, I., and Oppenheim, A.B. 1989. Control of plant diseases by chitinases expressed from cloned DNA in Escherichia coli. Phytopathology, 79: 1246-1249.
26. Shternshis, M.V., Beljaev, A.A., Shpatova, T.V., Bokova, J.V., and Duzhak, A.B. 2002. Field testing of BACTICIDE®, PHYTOVERM® and CHITINASE for control of the raspberry midge blight in Siberia. Biocontrol, 47: 697-706.
27. Subrahmanyam, P., Moss, J.P., and Rao, V.R. 1983. Resistance to peanut rust in wild Arachis species. Plant Dis. 67: 209-212.
28. Zhang, Z., and Yuen, G.Y. 2000. The role of chitinase production by Stenotrophomonas maltophilia strain C3 in biological control of Bipolaris sorokiniana. Phytopathology, 90: 384-389.