Development of a psilocin immunoassay for serum and blood samples

International Journal of Legal Medicine

Volumn 118, Issue 6, 2004, Pages 326-331

  Albers, C ^a   Kohler H ^b   Lehr, M ^a   Brinkmann, B ^b   Beike, J ^b  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b UNIVERSITY HOSPITAL MÜNSTER   (Germany)

Author keywords

GC MS; Psilocin; Radioimmunoassay

Indexed keywords

AMPHETAMINE; ANTIGEN; COCAINE; DRONABINOL; MORPHINE; POLYCLONAL ANTISERUM; PSILOCIN; SEROTONIN; TRICYCLIC ANTIDEPRESSANT AGENT; TRITIUM; TRYPTOPHAN; TYROSINE;

ANIMAL EXPERIMENT; ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; CROSS REACTION; GAS CHROMATOGRAPHY; INTERMETHOD COMPARISON; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; RABBIT; RADIOIMMUNOASSAY; SERUM;

ANIMALS; HALLUCINOGENS; HUMANS; PSILOCYBINE; RABBITS; RADIOIMMUNOASSAY; SENSITIVITY AND SPECIFICITY; SUBSTANCE ABUSE DETECTION;

EID: 11144325019     PISSN: 09379827     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00414-004-0469-9     Document Type: Article

References (19)

1. Passie T, Seifert J, Schneider U, Emrich HM (2002) The pharmacology of psilocybin. Addict Biol 7:357-364
2. Horita A, Weber LJ (1961) The enzymic dephosphorylation and oxidation of psilocybin and psilocin by mammalian tissue homogenates. Biochem Pharmacol 7:47-54
3. Horita A, Weber LJ (1962) Dephosphorylation of psilocybin in the intact mouse. Toxicol Appl Pharmacol 4:730-737
4. Kalberer F, Kreis W, Rutschmann J (1962) The fate of psilocin in the rat. Biochem Pharmacol 11:261-269
5. Hasler F, Bourquin D, Brenneisen R, Baer T, Vollenweider FX (1997) Determination of psilocin and 4-hydroxyindole-3-acetic acid in plasma by HPLC-ECD and pharmacokinetic profiles of oral and intravenous psilocybin in man. Pharma Acta Helv 72:175-184
6. Sticht G, Käferstein H (2000) Detection of psilocin in body fluids. Forensic Sci Int 113:403-407
7. Kysilka R (1990) Determination of psilocin in rat urine by high-performance liquid chromatography with electrochemical detection. J Chromatogr 534:287-290
8. Lindenblatt H, Kramer E, Holzmann-Erens P, Gouzoulis-Mayfrank E, Kovar K-A (1998) Quantitation of psilocin in human plasma by high-performance liquid chromatography and electrochemical detection: comparison of liquid-liquid extraction with automated online solid-phase extraction. J Chromatogr B Biomed Sci Appl 709:255-263
9. Grieshaber AF, Moore KA, Levine B (2001) The detection of psilocin in human urine. J Forensic Sci 46:627-630
10. Bogusz MJ (2000) Liquid chromatography-mass spectrometry as a routine method in forensic sciences: a proof of maturity. J Chromatogr B Biomed Sci Appl 748:3-19
11. Kamata T, Nishikawa M, Katagi M, Tsuchihashi H (2003) Optimized glucuronide hydrolysis for the detection of psilocin in human urine samples. J Chromatogr B Analyt Technol Biomed Life Sci 792:421-427
12. Sagawa K, Kimura A, Saito Y (2003) Production and characterization of a monoclonal antibody for sweat-specific protein and its application for sweat identification. Int J Legal Med 117:90-95
13. Pavlic M, Haidekker A, Grubwieser P (2002) Fatal accident caused by isoflurane abuse. Int J Legal Med 116:357-360
14. Albers C, Lehr M, Beike J, Kohler H, Brinkmann B (2002) Synthesis of a psilocin hapten and a protein-hapten conjugate. J Pharm Pharmacol 54:1265-1270
15. Nichols DE, Frescas S (1999) Improvements to the synthesis of psilocybin and a facile method for preparing the O-acetal prodrug of psilocin. Synthesis 6:935-938
16. Yamada F, Tamura M, Somei M (1998) A facile synthesis of psilocin from indole-3-carbaldehyde. Heterocycles 49:451-457
17. Abraham GE (1969) Solid phase radioimmunoassay of estradiol-17 beta. J Clin Endocrinol Metab 29:866-870
18. Troxler F, Seemann F, Hofmann A (1959) Synthetic indole compounds. II. Psilocybin and psilocin modifications. Helv Chim Acta 42:2073-2103
19. U.S. Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research, Medicine CfV (2001) Guidance for industry. Bioanalytical validation