Activity-dependent internalization of smoothened mediated by β-Arrestin 2 and GRK2

Science

Volumn 306, Issue 5705, 2004, Pages 2257-2260

  Chen, Wei ^a   Ren, Xiu Rong ^a   Nelson, Christopher D ^a   Barak, Larry S ^a   Chen, James K ^b,d   Beachy, Philip A ^b   De Sauvage, Frederic ^c   Lefkowitz, Robert J ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c GENENTECH INC   (United States)

^d STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; GROWTH KINETICS; MOLECULAR DYNAMICS; PROTEINS; SIGNALING;

ENDOCYTOSIS; MOLECULAR INTERACTIONS; PHOSPHORYLATION; VERTEBRATE DEVELOPMENTAL PROCESSES;

CELL MEMBRANES;

BETA ARRESTIN; BETA ARRESTIN 2; CLATHRIN; CYCLOPAMINE; G PROTEIN COUPLED RECEPTOR; G PROTEIN COUPLED RECEPTOR 2; GREEN FLUORESCENT PROTEIN; PROTEIN; PROTEIN INHIBITOR; PROTEIN PATCHED; PROTEIN SMO; SONIC HEDGEHOG PROTEIN; UNCLASSIFIED DRUG;

BIOLOGY;

ARTICLE; CONTROLLED STUDY; EMBRYO; ENDOCYTOSIS; HUMAN; HUMAN CELL; INTERNALIZATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION; VERTEBRATE;

ARRESTINS; BETA-ADRENERGIC RECEPTOR KINASE; CELL LINE; CELL MEMBRANE; CLATHRIN; COATED PITS, CELL-MEMBRANE; CYCLIC AMP-DEPENDENT PROTEIN KINASES; CYCLOHEXYLAMINES; CYTOSOL; DYNAMINS; ENDOCYTOSIS; HEDGEHOG PROTEINS; HUMANS; MEMBRANE PROTEINS; PHOSPHORYLATION; RECEPTORS, CELL SURFACE; RECEPTORS, G-PROTEIN-COUPLED; RECOMBINANT FUSION PROTEINS; SIGNAL TRANSDUCTION; THIOPHENES; TRANS-ACTIVATORS; TRANSFECTION; VERATRUM ALKALOIDS;

ERINACEIDAE; MAMMALIA; VERTEBRATA;

EID: 11144256194     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1104135     Document Type: Article

References (31)

1. M. M. Cohen Jr., Am. J. Med. Genet. 123A, 5 (2003).
2. P. W. Ingham, A. P. McMahon, Genes Dev. 15, 3059 (2001).
3. J. Xie et al., Nature 391, 90 (1998).
4. R. Wechsler-Reya, M. P. Scott, Annu. Rev. Neurosci. 24, 385 (2001).
5. D. M. Berman et al., Nature 425, 846 (2003).
6. D. N. Watkins et al., Nature 422, 313 (2003).
7. S. P. Thayer et al., Nature 425, 851 (2003).
8. L. M. Luttrell, R. J. Lefkowitz, J. Cell Sci. 115, 455 (2002).
9. J. A. Pitcher, N. J. Freedman, R. J. Lefkowitz, Annu. Rev. Biochem. 67, 653 (1998).
10. J. Taipale et al., Nature 406, 1005 (2000).
11. M. Hynes et al., Nature Neurosci. 3, 41 (2000).
12. L. S. Barak, S. S. Ferguson, J. Zhang, M. G. Caron, J. Biol. Chem. 272, 27497 (1997).
13. R. H. Oakley, S. A. Laporte, J. A. Holt, L. S. Barak, M. G. Caron, J. Biol. Chem. 276, 19452 (2001).
14. J. Taipale, M. K. Cooper, T. Maiti, P. A. Beachy, Nature 418, 892 (2002).
15. W. Chen et al., data not shown.
16. R. B. Pepinsky et al., J. Biol. Chem. 273, 14037 (1998).
17. N. Denef, D. Neubuser, L. Perez, S. M. Cohen, Cell 102, 521 (2000).
18. J. K. Chen, J. Taipale, K. E. Young, T. Maiti, P. A. Beachy, Proc. Natl. Acad. Sci. U.S.A. 99, 14071 (2002).
19. Materials and methods are available as supporting material on Science Online.
20. P. H. McDonald et al., Science 290, 1574 (2000).
21. L. M. Luttrell, J. Mol. Endocrinol. 30, 117 (2003).
22. A. M. Wilbanks et al., Science 306, 2264 (2004).
23. F. J. van Eeden et al., Development 123, 153 (1996).
24. M. J. Barresi, H. L. Stickney, S. H. Devoto, Development 127, 2189 (2000).
25. W. Chen, S. Burgess, N. Hopkins, Development 128, 2385 (2001).
26. D. L. DeCamp, T. M. Thompson, F. J. de Sauvage, M. R. Lerner, J. Biol. Chem. 275, 26322 (2000).
27. L. Ruel, R. Rodriguez, A. Gallet, L. Lavenant-Staccini, P. P. Therond, Nature Cell Biol. 5, 907 (2003).
28. S. K. Ogden et al., Curr. Biol. 13, 1998 (2003).
29. J. Jia, C. Tong, J. Jiang, Genes Dev. 17, 2709 (2003).
30. L. Lum et al., Mol. Cell 12, 1261 (2003).
31. We thank M. Caron, R. Wechsler-Reya, and A. Meloni for critical discussion and reading of the manuscript; H. Kendall and L. Langevin for technical assistance; and E. Hall and D. Addison for secretarial assistance. Supported in part by NIH grant HL 16037 (R.J.L.), HL 61365 (L.S.B.). R.J.L. and P.A.B. are Investigators of the Howard Hughes Medical Institute.