On-chip PCR amplification of very long templates using immobilized primers on glassy surfaces

Biosensors and Bioelectronics

Volumn 20, Issue 8 SPEC. ISS., 2005, Pages 1491-1498

  Nickisch Rosenegk, Markus Von ^a   Marschan, Xenia ^a   Andresen, Dennie ^a   Abraham, Alexandra ^a   Heise, Christian ^a   Bier, Frank F ^a  

^a FRAUNHOFER INSTITUTE FOR BIOMEDICAL ENGINEERING   (Germany)

Author keywords

DNA templates; Immobilized primers; PCR amplification

Indexed keywords

AMPLIFICATION; DNA; ENZYMES; FLUORESCENCE; MICROPROCESSOR CHIPS; OPTICAL MICROSCOPY; SCANNING; SYNTHESIS (CHEMICAL);

CONFOCAL MICROARRAY SCANNING; FLUORESCENCE MICROSCOPY; MITOCHONDRION; POLYMERASE CHAIN REACTIONS (PCR);

GENETIC ENGINEERING;

DNA FRAGMENT; DOUBLE STRANDED DNA; DYE; GLASS; INTERCALATING AGENT; PRIMER DNA;

BACTERIOPHAGE; BLOOD SAMPLING; BLOOD VOLUME; CAPILLARY; CHEMICAL MODIFICATION; CONFERENCE PAPER; CONFOCAL MICROSCOPY; DNA BINDING; DNA MICROARRAY; DNA TEMPLATE; FLUORESCENCE MICROSCOPY; GENE AMPLIFICATION; GENOME; MITOCHONDRION; NUCLEIC ACID IMMOBILIZATION; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SURFACE PROPERTY; SUSPENSION; SYNTHESIS;

EID: 11144226239     PISSN: 09565663     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bios.2004.07.007     Document Type: Conference Paper

References (18)

1. C. Adessi, G. Matton, G. Ayala, G. Turcatti, J.J. Mermod, P. Mayer, and E. Kawashima Solid phase DNA amplification: characterization of primer attachment and amplification mechanisms Nucl. Acid Res. 28 20 2000 87
2. F.F. Bier, F. Kleinjung, P.M. Schmidt, and F.W. Scheller Determination of the turnover number of the restriction endonuclease EcoRI using evanescent wave technology Anal. Bioanal. Chem. 372 2 2002 308 313
3. Bier, F.F., Gajovic-Eichelmann, N., Hölzel, R., 2002b. Oriented immobilization of single DNA molecules as a nanostructuring tool. In: Fritzsche, W. (Ed.), DNA-based Molecular Construction, AIP Conference Proceedings 640 (2002) 51-59.
4. F.F. Bier, E. Ehrentreich-Förster, and P.M. Schmidt Real-time analysis on microarrays Anal. Bioanal. Chem. 378 1 2004 52 53
5. M. Buckle, R.M. Williams, M. Negroni, and H. Buc Real time measurements of elongation by a reverse transcriptase using surface plasmon resonance 1996
6. F. Erdogan, R. Kirchner, W. Mann, H. Ropers, and U.A. Nuber Detection of mitochondrial single nucleotide polymorphism using a primer elongation reaction on oligonucleotide microarrays Nucl. Acid Res. 29 7 2001 36
7. D.G. Higgins, and P.M. Sharp CLUSTAL: a package for performing multiple sequence alignment on a microcomputer Gene 73 1 1988 237 244
8. R. Hölzel, N. Gajovic-Eichelmann, and F.F. Bier Oriented and vectorial immobilization of linear M13 dsDNA between interdigitated electrodes - towards single molecule DNA nanostructures Biosens. Bioelectron. 18 2003 555 564
9. M. Huber, D. Losert, R. Hiller, C. Harwanegg, M.W. Mueller, and W.M. Schmidt Detection of single base alterations in genomic DNA by solid phase polymerase chain reaction on oligonucleotide microarrays Anal. Biochem. 299 2001 24 30
10. M. Huber, A. Mündlein, E. Dornstauder, C. Schneeberger, C.B. Tempfer, M.W. Mueller, and W.M. Schmidt Accessing single nucleotide polymorphisms in genomic DNA by direct multiplex polymerase chain reaction. Amplification on oligonucleotide microarrays Anal. Biochem. 303 2002 25 33
11. M. Kakuta, F.G. Bessoth, and A. Manz Microfabricated devices for fluid mixing and their application for chemical synthesis Chem. Rec. 1 5 2001 395 405
12. Kleinjung, F. 1998. Sensorik und Kinetik biochemischer Nukleinsäure-Wechselwirkungen an Oberflächen. Dissertation. Math.-Nat. Fakultät., Univ. Potsdam, p. 58
13. D.M. Mitra, and G.M. Church In situ localized amplification and contact replication of many individual DNA molecules Nucl. Acid Res. 27 1999 e34
14. P.M. Schmidt, C. Lehmann, E. Mattes, and F.F. Bier Detection of activity of telomerase in tumor cells using fibre optical biosensors Biosens. Bioelectron. 17 2002 1081 1087
15. E. Southern, K. Mir, and M. Shchepinov Molecular interactions on microarrays Nature Genet. Suppl. 21 1999 5 9
16. Strizhkov, B.N., Drobyshev, A.L., Mikhailovic, V.M., Mirzabekov, A.D., 2000. PCR amplification on a microarray of gel-immobilized oligonucleotides; detection of bacterial toxin- and drug-resistant genes and their mutations. Biotechniques 29 (4), 844-848, 850-852, 854.
17. G.J. Violeta, and H.E. Swaisgood Analysis and optimization of methods using water-soluble carbodiimide for immobilization of biochemicals to porous glass Bioengeneering 24 1982 1069 1080
18. A.T. Woolley, D. Hadley, P. Landre, A.J. deMello, R.A. Mathies, and M.A. Northrup Functional integration of PCR amplification and capillary electrophoresis in a microfabricated DNA analysis device Anal. Chem. 68 23 1996 4081 4086