Transfection efficiency and toxicity following delivery of naked plasmid DNA and cationic lipid-DNA complexes to ovine lung segments

Molecular Therapy

Volumn 8, Issue 4, 2003, Pages 646-653

  Emerson, Michael ^a,b,c,h   Renwick, Louise ^a,b   Tate, Stephen ^a,b   Rhind, Susan ^c   Milne, Elspeth ^c   Painter, Hazel ^b,d   Boyd, A Christopher ^a,b   McLachlan, Gerry ^a,b   Griesenbach, Uta ^b,e   Cheng, Seng H ^f   Gill, Deborah R ^b,d   Hyde, Stephen C ^b,d   Baker, Alison ^b,g   Alton, Eric W ^b,e   Porteous, David J ^a,b   Collie, D David S ^b,g,i  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c UNIVERSITY OF EDINBURGH   (United Kingdom)

^d UNIVERSITY OF OXFORD   (United Kingdom)

^e IMPERIAL COLLEGE LONDON   (United Kingdom)

^f GENZYME CORPORATION   (United States)

^g UNIVERSITY OF EDINBURGH   (United Kingdom)

^h UNIVERSITY OF MANCHESTER   (United Kingdom)

ⁱ UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Cationic lipid; Chloramphenicol acetyltransferase; Cystic fibrosis; Domestic; Gene transfer; Genetic vectors; Lung; Messenger; RNA; Sheep; Toxicology

Indexed keywords

ANTHELMINTIC AGENT; CATION; CHLORAMPHENICOL ACETYLTRANSFERASE; LIPID; LIPOSOME; MACROGOL DERIVATIVE; MESSENGER RNA; NAKED DNA; PLASMID DNA;

AIRWAY; ANESTHESIA; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; AUTOPSY; COMPLEX FORMATION; CONTROLLED STUDY; CORRELATION FUNCTION; DISEASE SEVERITY; DNA TRANSFECTION; DOSE RESPONSE; DRUG EFFICACY; DRUG INSTILLATION; EUTHANASIA; HISTOPATHOLOGY; LUNG PARENCHYMA; NEUTROPHIL; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PROTEIN EXPRESSION; RESPIRATORY TRACT INFLAMMATION; SAMPLING; SHEEP; TOXICITY TESTING;

ANIMALIA; FELIS CATUS; OVIS; OVIS ARIES;

EID: 10744228492     PISSN: 15250016     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1525-0016(03)00233-8     Document Type: Article

References (28)

1. Bouljihad, M., and Leipold, H. W. (1994). An ultrastructural study of pulmonary bronchiolar and alveolar epithelium in sheep. Zentralbl. Veterinarmed. A 41: 573-586.
2. Olver, R. E., and Robinson, E. J. (1986). Sodium and chloride transport by the tracheal epithelium of fetal, new-born and adult sheep. J. Physiol. 375: 377-390.
3. Tebbutt, S. J., Wardle, C. J., Hill, D. F., and Harris, A. (1995). Molecular analysis of the ovine cystic fibrosis transmembrane conductance regulator gene. Proc. Natl. Acad. Sci. USA 92: 2293-2297.
4. Wilmut, I., Schnieke, A. E., McWhir, J., Kind, A. J., and Campbell, K. H. (1997). Viable offspring derived from fetal and adult mammalian cells. Nature 385: 810-813.
5. Campbell, K. H., McWhir, J., Ritchie, W. A., and Wilmut, I. (1996). Sheep cloned by nuclear transfer from a cultured cell line. Nature 380: 64-66.
6. McWhir, J., et al. (1996). Selective ablation of differentiated cells permits isolation of embryonic stem cell lines from marine embryos with a non-permissive genetic background. Nat. Genet. 14: 223-226.
7. Collie, D. D., et al. (2001). Local lung responses following local lung challenge with recombinant lungworm antigen in systemically sensitized sheep. Clin. Exp. Allergy 31: 1636-1647.
8. Ferrari, S., et al. (2001). Mucus altering agents as adjuncts for nonviral gene transfer to airway epithelium. Gene Ther. 8: 1380-1386.
9. Bice, D. E., King-Herbert, A. P., Morris, M. J., Hanna, N., and Haley, P. J. (1989). inflammation and recruitment of immune cells into the lung. Region. Immunol. 2: 376-384.
10. Gratziou, C., Carroll, M., Montefort, S., Teran, L., Howarth, P. H., and Holgate, S. T. (1996). Inflammatory and T-cell profile of asthmatic airways 6 hours after local allergen provocation. Am. J. Respir. Crit. Care Med. 153: 515-520.
11. Hohlfeld, J. M., et al. (1999). Dysfunction of pulmonary surfactant in asthmatics after segmental allergen challenge. Am. J. Respir. Crit. Care Med. 159: 1803-1809.
12. Kane, G. C., et al. (1996). A controlled trial of the effect of the 5-lipoxygenase inhibitor, zileuton, on lung inflammation produced by segmental antigen challenge in human beings. J. Allergy Clin. Immunol. 97: 646-654.
13. Shaver, J. R., et al. (1995). Pulmonary inflammation after segmental ragweed challenge in allergic asthmatic and nonasthmatic subjects, Am. J. Respir. Crit. Care Med. 152: 1189-1197.
14. + T cells. J. Immunol. 157: 2160-2165.
15. Sur, S., Kita, H., Gleich, G. J., Chenier, T. C., and Hunt, L. W. (1996). Eosinophil recruitment is associated with IL-5, but not with RANTES, twenty-four hours after allergen challenge, J. Allergy Clin. Immunol. 97: 1272-1278.
16. Weissman, D. N., Bice, D. E., Crowell, R. E., and Schuyler, M. R. (1994). Intrapulmonary antigen deposition in the human lung: Local responses. Am. J. Respir. Cell. Mol. Biol. 11: 607-614.
17. Georas, S. N., Liu, M. C., Newman, W., Beall, L. D., Stealey, B. A., and Bochner, B. S. (1992). Altered adhesion molecule expression and endothelial cell activation accompany the recruitment of human granulocytes to the lung after segmental antigen challenge. Am. J. Respir. Cell. Mol. Biol. 7: 261-269.
18. Julius, P., Luttmann, W., Knoechel, B., Kroegel, C., Matthys, H., and Virchow, J. C. Jr (1999). CD69 surface expression on human lung eosinophils after segmental allergen provocation. Eur. Respir. J. 13: 1253-1259.
19. Hunt, L. W., et al. (2002). Removal of bronchoalveolar cells augments the late eosinophilic response to segmental allergen challenge. Clin. Exp. Allergy 32: 210-216.
20. Out, T. A., Wang, S. Z., Rudolph, K., and Bice, D. E. (2002). Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs. Immunology 105: 499-508.
21. Erpenbeck, V. J., et al. (2003). Local release of eosinophil peroxidase following segmental allergen provocation in asthma. Clin. Exp. Allergy 33: 331-336.
22. Rose, A. C., Goddard, C. A., Colledge, W. H., Cheng, S. H., Gill, D. R., and Hyde, S. C. (2002). Optimisation of real-time quantitative RT-PCR for the evaluation of non-viral mediated gene transfer to the airways. Gene Ther. 9: 1312-1320.
23. Scheule, R. K., et al. (1997). Basis of pulmonary toxicity associated with cationic lipid-mediated gene transfer to the mammalian lung. Hum. Gene Ther. 8: 689-707.
24. Eastman, S. J., et al. (1997). A concentrated and stable aerosol formulation of cationic lipid: DNA complexes giving high-level gene expression in mouse lung. Hum. Gene Ther. 8: 765-771
25. Alton, E. W., et al. (1999). Cationic lipid-mediated CFTR gene transfer to the lungs and nose of patients with cystic fibrosis: a double-blind placebo-controlled trial. Lancet 353: 947-954.
26. Yew, N. S., et al. (1999). Contribution of plasmid DNA to inflammation in the lung after administration of cationic lipid: pDNA complexes. Hum. Gene Ther. 10: 223-234.
27. Cunningham, S., Meng, Q. H., Klein, N., McAnulty, R. J., and Hart, S. L. (2002). Evaluation of a porcine model for pulmonary gene transfer using a novel synthetic vector. J. Gene Med. 4: 438-446.
28. Zabner, J., et al. (1997). Comparison of DNA-lipid complexes and DNA alone for gene transfer to cystic fibrosis airway epithelia in vivo. J. Clin. Invest. 100: 1529-1537.