Individual patient-dependent influence of erythrocyte lysing procedures on flow-cytometric analysis of leukocyte subpopulations

Transfusion Medicine and Hemotherapy

Volumn 30, Issue 4, 2003, Pages 165-170

  Gohde W ^a   Cassens, U ^b   Lehman, L G ^c   Traore Y ^d   Gohde Jr W ^e   Berkes, P ^a   Westerberg, C ^b   Greve, B ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b UNIVERSITY HOSPITAL MÜNSTER   (Germany)

^c UNIVERSITY OF DOUALA   (Cameroon)

^d UNIVERSITÉ DE OUAGADOUGOU   (Burkina Faso)

^e Partec GmbH   (Germany)

Author keywords

CD4+ T cells; Flow cytometry; Individual lysing loss

Indexed keywords

CD4 ANTIGEN;

ACCURACY; ANALYTIC METHOD; ARTICLE; BLOOD SAMPLING; CELL LOSS; CELL SUBPOPULATION; CONTROLLED STUDY; ERYTHROCYTE; FLOW CYTOMETRY; HEMOLYSIS; HUMAN; INDIVIDUALITY; LEUKOCYTE; OBSERVER VARIATION; REPRODUCIBILITY; T LYMPHOCYTE;

EID: 10744223281     PISSN: 16603796     EISSN: None     Source Type: Journal    
DOI: 10.1159/000073325     Document Type: Article

References (22)

1. Helbert M, Breuer J: Monitoring patients with HIV disease. J Clin Pathol 2000;53:266-272.
2. Brando B, Barnett D, Janossy G, Mandy F, Autran B, Rothe G, Scarpati B, D'Avanzo G, D'Hautcourt J-L, Lenkei R, Schmitz G, Kunkl A, Chianese R, Papa S, Gratama J-W, for the European Working Party on Clinical Cell Analysis (EWGCCA): Cytofluorometric methods for assessing absolute numbers of cell subsets in blood. Cytometry 2000;42:327-346.
3. Barnett D, Granger V, Whitby L, Storie I, Reilly JT: Absolute CD4+ T-lymphocyte and CD34+ stem cell counts by single-platform flow cytometry: The way forward. Br J Haematol 1999;106:1059-1062.
4. Connelly MC, Knight M, Giorgi JV: Standardization of absolute CD4+ lymphocyte counts across laboratories: An evaluation of the Ortho Cytoron-Absolute flow cytometry system on normal donors. Cytometry 1995;22:200-210.
5. Cassens U, Gutensohn K, Garritsen H, Kelsch R, Kuehnl P, Sibrowski W: The influence of different erythrocyte lysing procedures on flow cytometric determination of CD34+ cells in umbilical cord blood transplants. Transfus Med 1998;8:111-118.
6. Greve B, Beller C, Cassens U, Sibrowski W, Severin E, Göhde W: High grade loss of leukocytes and hematopoietic progenitor cells caused by erythrocyte lysing procedures for flow cytometric analyses. J Hematother Stem Cell Res 2003;12:321-330.
7. Cassens U, Greve B, Tapanon K, Nave B, Severin E, Sibrowski W, Göhde W: A novel true volumetric method for the determination of residual leucocytes in blood components. Vox Sang 2002;82:198-206.
8. Greve B, Cassens U, Westerberg C, Göhde W jun, Sibrowski W, Reichelt D, Göhde W: A new no-lyse, no-wash flow-cytometric method for the determination of CD4 T cells in blood samples. Transfus Med Hemother 2003;30:8-13 .
9. Centers for Disease Control: Guidelines for the use of antiretroviral agents in HIV-infected adults and adolescents. Morbid Mortal Weekly Rep 1992;47:1-91.
10. Fahey JL, Taylor TMG, Detels R, Hofmann B, Melmed R, Nishanian P, Giorgi JV: The prognostic value of cellular and serologic markers in infection with human immunodeficiency virus type 1. N Engl J Med 1990;322:166-172.
11. Landay A, Ho JL, Hom D, Russell T, Zwerner R, Minuty JG, Kataaha P, Mmiro F, Jackson B: A rapid manual method for CD4+ T-cell quantitation for use in developing countries. AIDS 1993;7:1565-1568.
12. Johnson D, Hirschkorn D, Busch MP: National Heart, Lung, and Blood Institute Retrovirus Epidemiology Donor Study: Evaluation of four alternative methodologies for determination of absolute CD4+ lymphocyte counts. J Acquir Immune Defic Syndr Hum Retrovirol 1995;10:522-530.
13. McCoy JP, Blumstein L, Donaldson MH, Baxter JD, Byrne BC, Gekowski KM: Accuracy and cost-effectiveness of a one-tube, three color method for obtaining absolute CD4 counts and CD4:CD8 ratios. Hematopathology 1993;103:279-282.
14. Janossy G, Jani IV, Goehde W: Affordable CD4+ T cell counts on 'single-platform' flow cytometers. I. Primary CD4 gating. Br J Haematol 2000;111:1198-1208.
15. Kreissig C, Kirsch A, Serke S: Characterization and measurement of CD34-expressing hematopoietic cells. J Hematother 1994;3:263-290.
16. Bender JG, Unverzagt K, Walker D: Guidelines for determination of CD34+ cells by flow cytometry: Application to the harvesting and transplantation of peripheral blood stem cells; in Wunder E, Sovalat H, Henon P, Serke S (eds): Hematopoietic Stem Cells. Dayton, AlphaMed Press, 1994, pp 31-43.
17. Gratama JW, Menéndez P, Kraan J, Orfao A: Loss of CD34+ hematopoietic progenitor cells due to washing can be reduced by the use of fixative-free erythrocyte lysing reagents. J Immunol Methods 2000;239:13-23.
18. Alvarez-Larran A, Jover L, Marin P, Petriz J: A multicolor, no-lyse no-wash assay for the absolute counting of CD34+ cells by flow cytometry. Cytometry 2002,5:249-253.
19. Dhabhar FS, Miller AH, McEwen BS, Spencer RL: Effects of stress on immune cell distribution. Dynamics and hormonal mechanisms. J Immunol 1995;154:5511-5527.
20. Infante JR, Peran F, Martinez M, Poyatos R, Roldan A, Ruiz C, Garrido F: Lymphocyte subpopulations and catecholamines: Daytime varyations and relationships. Rev Esp Fisiol 1996;52:143-148.
21. Kaplan G, Britton WJ, Hancock GE, Theuvenet WJ, Smith KA, Job CK, Roche PW, Molloy A, Burkhardt R, Barker J, Pradhan HM, Cohn ZA: The systemic influence of recombinant interleukin 2 on the manifestations of lepromatous leprosy. J Exp Med 1991;173:993-1006.
22. Giorgi JV: Characterization of T lymphocyte subset alterations by flow cytometry in HIV disease. Ann N Y Acad Sci 1993;677:126-137.