Simplified desalting of ligation reactions immediately prior to electroporation into E. coli

BioTechniques

Volumn 21, Issue 6, 1996, Pages 1024-

  Atrazhev, Alexey M ^a   Elliott, John F ^a,b  

^a UNIVERSITY OF ALBERTA   (Canada)

^b ALBERTA ENVIRONMENT AND PARKS   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; PLASMID DNA;

ARTICLE; BACTERIUM TRANSFORMATION; CHEMICAL REACTION; ELECTROPORATION; ESCHERICHIA COLI; GENETIC TRANSFORMATION; LIGATION; MOLECULAR CLONING; NONHUMAN;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 10544253076     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96216bm12     Document Type: Article

References (5)

1. Dower, W.J., J.F. Miller and C.W. Ragsdale. 1988. High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res. 16:6127-6145.
2. Miller, J.F., W.J. Dower and C.W. Ragsdale. 1988. High voltage electroporation of bacteria: genetic transformation of Campylobacter jejuni with plasmid DNA. Proc. Natl. Acad. Sci. USA 85:856-860.
3. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
4. Shizuya, H., B. Birren, U.-J. Kim, V. Mancino, T. Slepak, Y. Tachiiri and M. Simon. 1992. Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc. Natl. Acad. Sci. USA 89:8794-8797.
5. Silhavy, T.J., M.L. Berman and L.W. Enquist. 1984. Experiments with Gene Fusion. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.