The antiplatelet effects of a new nitroderivative of acetylsalicylic acid - An in vitro study of inhibition on the early phase of platelet activation and on TXA2 production

Thrombosis and Haemostasis

Volumn 76, Issue 5, 1996, Pages 791-798

  Lechi, Clara ^a,b   Andrioli, Giuseppe ^a   Gaino, Stefania ^a   Tommasoli, Rosamaria ^a   Zuliani, Valeria ^a   Ortolani, Riccardo ^a   Degan, Maurizio ^a   Benoni, Giuseppina ^a   Bellavite, Paolo ^a   Lechi, Alessandro ^a   Minuz, Pietro ^a  

^a UNIVERSITY OF VERONA   (Italy)

^b POLICLINICO BORGO ROMA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

2 ACETOXYBENZOATE 2 (1 NITROXYMETHYL)PHENYL ESTER; ACETYLSALICYLIC ACID 3 (NITROXYMETHYL)PHENYL ESTER; ACETYLSALICYLIC ACID DERIVATIVE; ANTITHROMBOCYTIC AGENT; ARACHIDONIC ACID; CALCIUM; CYCLIC GMP; FIBRINOGEN RECEPTOR; FURA 2; GUANYLATE CYCLASE; METHYLENE BLUE; NITRIC OXIDE; OXYHEMOGLOBIN; PADGEM PROTEIN; PROSTAGLANDIN SYNTHASE; THROMBOXANE A2; UNCLASSIFIED DRUG;

ARTICLE; CALCIUM CELL LEVEL; CONTROLLED STUDY; DRUG EFFECT; ENZYME ACTIVATION; ENZYME INHIBITION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; NORMAL HUMAN; PRIORITY JOURNAL; THROMBOCYTE ACTIVATION; THROMBOCYTE ADHESION; THROMBOCYTE AGGREGATION;

EID: 10544234611     PISSN: 03406245     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-0038-1650662     Document Type: Article

References (43)

1. Ignarro LJ, Ross G, Tillisch J. Pharmacology of endothelium-derived nitric oxide and nitrovasodilators. West J Med 1991; 154: 51-62.
2. Stamler JS, Loscalzo J. The antiplatelet effects of organic nitrates and related compounds in vitro and in vivo and their relevance to cardiovascular disorders. J Am Coll Cardiol 1991; 18: 529-36.
3. Wallace JL, Reuter B, Cicala C, McKnight W, Grisham NB, Cirino G. Novel NSAID derivatives with markedly reduced ulcerogenic properties in the rat. Gastroenterol 1994; 107: 173-9.
4. Lechi C, Minuz P, Gaino S, Tommasoli R, Del Soldato P, Benoni G, Zuliani V, Lechi A. Antiaggregating and vasodilatory effects of a new nitroderivative of acetylsalicylic acid. Eur J Clin Invest 1995; 25: A 234.
5. Minuz P, Lechi C, Tommasoli R, Gaino S, Degan M, Zuliani V, Bonapace S, Benoni G, Adami A, Cuzzolin L, Lechi A. Antiaggregating and vasodilatory effects of a new nitroderivative of acetylsalicylic acid. Thromb Res 1995; 80: 367-76.
6. Shattil SJ, Cunningham M, Hoxie JA. Detection of activated platelets in whole blood using activation dependent monoclonal antibodies and flow cytometry. Blood 1987; 70: 307-15.
7. Hallam TJ, Thompson NT, Scrutton MC, Rink TJ. The role of cytoplasmic free calcium in the responses of Quin 2-loaded human platelets to vasopressin. Biochem J 1984; 221: 897-901.
8. Bellavite P, Andrioli G, Guzzo P, Arigliano P, Chirumbolo S, Manzalo F, Santonastaso C. A colorimetric method for the measurement of platelet adhesion in microtiter plates. Anal Biochem 1994; 216: 444-50.
9. Rajiv J, Engji H, Grunfeld S, Welch KMA. Dimethyl-sulfoxide and stimulation of platelet cytoplasmic calcium. Thromb Haemost 1989; 61: 326.
10. 3H arachidonic acid and changes in cytosolic free calcium in fura-2-loaded human platelets stimulated by ionomycin and collagen. Biochemical J 1986; 235: 869-77.
11. 2+ threshold and Ca-independent activation for shape change and secretion. FEBS Lett 1982; 148: 21-6.
12. Ault KA. Flow cytometric measurement of platelet function and reticulated platelets. Ann N Y Acad Sci 1993; 677: 293-308.
13. Chronos NAF, Wilson DJ, Janes SL, Hutton RA, Buller NP, Goodall AH. Aspirin does not affect the flow cytometryc detection of fibrinogen binding to, or release of α-granules or lysosomes from, human platelets. Clin Sci 1994; 87: 575-80.
14. Salvemini D, Korbut R, Änggard E, Vane J. Immediate release of a nitric oxide-like factor from bovine aortic endothelial cells by Escherichia coli lipopolysaccharide. Proc Natl Acad Sci USA 1990; 87: 2593-7.
15. Sils D, Rodgers SE, Lloyd JV, Wilson KM, Siebert DM, Bochner F. Inhibition of platelet aggregation and thromboxane production by low concentrations of aspirin in vitro. Clin Sci 1988; 74: 491-7.
16. Salvemini D, Pistelli A, Vane J. Conversion of glyceryl trinitrate to nitric oxide in tolerant and non-tolerant smooth muscle and endothelial cells. Br J Pharmacol 1993; 108: 162-9.
17. 2 production in healthy subjects. Thromb Res 1980; 17: 317-27.
18. Anderson TJ, Meredith IT, Ganz P, Selwyn AP, Yeung AC. Nitric oxide and nitrovasodilators: similarities, differences and potential interactions. J Am Coll Cardiol 1994; 24: 555-66.
19. Schafer AI, Alexander RW, Handin RI. Inhibition of platelet function by organic nitrate vasodilators.Blood 1980; 55: 649-54.
20. Mehta J, Mehta P. Comparative effects of nitroprusside and nitroglycerin on platelet aggregation in patients with heart failure. J Cardiovasc Pharmacol 1980; 2: 25-33.
21. Fitzgerald DJ, Roy L, Robertson RM, FitzGerald GA. The effects of organic nitrates on prostacyclin biosynthesis and platelet function in humans. Circulation 1984; 70: 297-302.
22. Stamler J, Cunnigham M, Loscalzo J. Reduced thiols and the effects intravenus nitroglycerin on platelet aggregation. Am J Cardiol 1988; 62: 377-80.
23. Diodati J, Theroux P, Latour JC, Lacoste L, Lam JYT, Waters D. Effects of nitroglycerin at therapeutic doses on platelet aggregation in unstable angina pectoris and acute myocardial infarction. J Am Coll Cardiol 1990; 17: 683-8.
24. Langford EJ, Brown AS, Wainwright RJ, de Belder AJ, Thomas MR, Smith REA, Radomski MW, Martin JF, Moncada S. Inhibition of platelet activity by S-nitroso-glutathione during coronary angioplasty. Lancet 1994; 344: 1458-60.
25. Buechler WA, Ivanova K, Wolfram G, Drummer C, Heim JM, Gerzer R. Soluble guanylyl cyclase and platelet function. Ann N Y Acad Sci 1994; 714: 151-7.
26. Ahlner J, Axelsson KL, Karlsson JOG, Andersson RGG. Glyceryl trinitrate inhibits phosphatidyl inositol hydrolysis and protein kinase C activity in bovine mesenteric artery. Life Sci 1988; 43: 1241-8.
27. Geiger J, Nolte C, Walter U. Regulation of calcium mobilization and entry in human platelets by endothelium-derived factors. Am J Physiol 1994; 267 (Cell Physiol 36): C236-C44.
28. 2+ entry in human platelets is resistant to nitric oxide. Biochem Biophys Res Commun 1995; 212: 90-6.
29. Larsen E, Celi A, Gilbert GE, Furie BC, Erban JK, Bonfanti R, Wagner DD, Furie B. PADGEM protein: a receptor that mediates the interaction of activated platelets with neutrophils and monocytes. Cell 1989; 59: 305-12.
30. Calvete JJ. Clues for understandig the structure and function of a prototypic human integrin: the platelet glycoprotein IIb/IIIa complex. Thromb Haemost 1994; 72: 1-15.
31. Ginsberg MH, Du X, Plow EF. Inside-out integrin signalling. Curr Opin Cell Biol 1992; 4: 766-71.
32. Stenberg PE, McEver RP, Shuman MA, Jacques YV, Bainton DF. A platelet alpha-granule membrane protein (GMP-140) is expressed on the plasma membrane after activation. J Cell Biol 1985; 101: 880-6.
33. Stamler JS, Singel DJ, Loscalzo J. Biochemistry of nitric oxide and its redox-activated forms. Science 1992; 258: 1898-902.
34. Gerzer R, Karrenbrock W, Siess W, Heim GM. Direct comparison of the effects of nitroprusside, SIN 1 and various nitrates on platelet aggregation and soluble guanylate-cyclase activity. Thromb Res 1988; 52: 11-21.
35. Salvemini D, Radzisewski W, Korbut R, Vane J. The use of oxyhaemoglobin to explore the events underlying inhibition of platelet aggregation induced by NO or NO-donors. Br J Pharmacol 1990; 101: 991-5.
36. Martin W, Villani GM, Jothianandan D, Furchgott RF. Selective blockade of endothelium-dependent and glyceryl-trinitrate-induced relaxation by hemoglobin and methylene blue in the rabbit aorta. J Pharmacol and Experim Ther 1985; 232: 708-16.
37. Durante W, Kroll MH, Vanhoutte PM, Sheffer AY. Endothelium-derived relaxing factor inhibits thrombin-induced platelet aggregation by inhibiting platelet phospholipase C. Blood 1992; 79: 110-6.
38. Tsai A. How does NO activate heme proteins? FEBS Lett 1994; 341: 141-5.
39. Salvemini D, Misko TP, Masferrer JR, Seibert K, Currie MG, Needleman P. Nitric oxide activates cyclo-oxygenase enzymes. Proc Natl Acad Sci 1993; 90: 7240-4.
40. Davidge ST, Baker PN, McLauglin MK, Roberts JM. Nitric oxide produced by endothelial cells increases production of eicosanoids through activation of prostaglandin H synthase. Circ Res 1995; 77: 274-83.
41. Stadler J, Harbrecht BG, Di Silvio M, Curran RD, Jordan ML, Simmons RL, Billiar TR. Endogenus nitric oxide inhibits the synthesis of cyclo-oxygenase products and interleukin-6 by rat Kupfer cells. J Leukoc Biol 1993; 53: 165-72.
42. Swierkosz TA, Mitchell JA, Warner TD, Botting RM, Vane JR. Coinduction of nitric oxide synthase and cyclo-oxygenase: interactions between nitric oxide and prostanoids. Br J Pharmacol 1995; 114: 1335-42.
43. 2 production in rats. Life Sci 1996; 58: 207-10.