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Volumn 274, Issue 5291, 1996, Pages 1363-1366

Melanoma cell expression of Fas(Apo-1/CD95) ligand: Implications for tumor immune escape

Author keywords

[No Author keywords available]

Indexed keywords

FAS ANTIGEN;

EID: 10544232277     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.274.5291.1363     Document Type: Article
Times cited : (1209)

References (41)
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    • (1994) Nature , vol.370 , pp. 218
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  • 12
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    • in preparation
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    • Hahne, M.1
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    • O. Beilgrau et al., Nature 377, 530 (1995).
    • (1995) Nature , vol.377 , pp. 530
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    • M. Hahne et al., data not shown
    • M. Hahne et al., data not shown.
  • 32
    • 10544249382 scopus 로고    scopus 로고
    • note
    • 6 melanoma cells were separated by SDS-PAGE (10%) under nonreducing conditions and immunoblotted (ECL system, Amersham) using the affinity-purified rabbit anti-FasL antibody PE62 (33).
  • 34
    • 10544237257 scopus 로고    scopus 로고
    • note
    • 5 labeled cells in a total volume of 200 μ1.
  • 35
    • 10544230136 scopus 로고    scopus 로고
    • note
    • 2 and 0.1% azide. Supernatants (1:1 dilution) of the monoclonal antibody (mAb) to FasL, A11 (33) (Alexis Corp., San Diego, CA) was added to the section for 1 hour, and after rinsing in PBS, peroxidase-conjugated goat antibody to rat Ig (TAGO, Burlingame, CA) was added for 0.5 hour. After rinsing, antibody location was revealed by the AEC chromogen. For the detection of melanoma cells, a mouse mAb to MAGE-1, detecting a subpopulation of melanoma cells (40), was used (20 μg/ml), and detected with a biotinylated goat anti-mouse Ig and avidin-peroxidase-conjugate (TAGO, Burlingame, CA). Fas antigen was detected with a mouse mAb to human Fas (10 μg/ml). In the negative control, the FasL antibody was replaced with rat Ig. The mAb to human melanoma (HMB45) was from ENZO Diagnostics Inc., New York, USA.
  • 37
    • 10544250993 scopus 로고    scopus 로고
    • note
    • Frozen sections were stained as in Fig. 2. T cells were detected using the anti-CD3 specific antibody leu-4 (Becton-Dickinson, Oxnard, CA). TUNEL staining was done according to kit instructions from Boehringer, Mannheim, Germany.
  • 38
    • 10544252202 scopus 로고    scopus 로고
    • note
    • 5, American Type Tissue Culture) were injected subcutaneously into 4-week old C57BL/6, CSTBL/6-lpr, C57Bl/6-gld (Jackson Laboratory, Bar Harbor, ME), MRL and MRL-lpr mice (Harian, Zeist, Nederland).
  • 41
    • 10544225690 scopus 로고    scopus 로고
    • note
    • We thank C. Kamel, F. Beerman, and K. Burns for helpful discussions; S. Hertig and S. Aslan for technical and secretarial assistance; and P. H. Krammer and F. Lejeune for reagents. Supported by grants of the Swiss National Science Foundation (J.T.) and of Human Frontier Science (M.H.). This study is dedicated to Dr. Stephan Carrel (Ludwig Institut of Cancer Research, Lausanne branch). With his death, melanoma research has lost a highly noted authority.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.