Design of Ebb-and-Flow Bioreactor (EFBR) for Immobilized "Hairy Root" Cultures: Part I. Preliminary Design Models and Culture Parameters

Transactions of the American Society of Agricultural Engineers

Volumn 46, Issue 5, 2003, Pages 1457-1468

  Cuello, J L ^a   Walker, P N ^b   Curtis, W R ^c  

^a University of Arizona   (United States)

^b University Park   (United States)

^c The Pennsylvania State University   (United States)

Author keywords

Bioreactor; Chemical production; Hairy roots; Secondary metabolites

Indexed keywords

MASS TRANSFER; MATHEMATICAL MODELS; METABOLISM; OXYGEN; SUGAR (SUCROSE);

LIQUID-PHASE DURATION; OXYGEN CONSUMPTION RATE (OCR);

BIOREACTORS;

BIOREACTOR; DESIGN;

HYOSCYAMUS; HYOSCYAMUS MUTICUS;

EID: 1042302521     PISSN: 00012351     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (50)

1. Benowitz, S. 1996. New technologies and approaches spur industry interest in plant-derived drugs. The Scientist 10(3): 1, 6-7. Available at: http://www.the-scientist.libraryupenn.edu/yr1996/feb/plant_96 0205.html.
2. Basu, P., and S. Chand. 1998. Tropane alkaloids from callus cultures, differentiated roots, and shoots of Hyoscyamus muticus L. J. Plant Biochemistry and Biotech. 7: 39-42.
3. Buitelaar, R. M., A. A. M. Langenhoff, R. Heidstra, and J. Tramper. 1991. Growth and thiophene production by "hairy root" cultures of Tagetes patula in various two-liquid-phase bioreactors. Enzyme Microb. Tech. 13: 487-494.
4. Cuello, J. L. 1994. Design and scale up of ebb-and-flow bioreactor (EFBR) for "hairy root" cultures. PhD diss. University Park, Pa.: Pennsylvania State University.
5. Cuello, J. L. 1999. Photosynthetic potential and polyacetylene production of Bidens and Acmella "hairy root" cultures. MS thesis. University Park, Pa.: Pennsylvania State University.
6. Cuello, J. L., P. N. Walker, and W. R. Curtis. 1991. Ebb-and-flow bioreactor for "hairy root" cultures. ASAE Paper No. 917528. St. Joseph, Mich.: ASAE.
7. Dawson, R. F. 1942. Nicotine synthesis in excised tobacco roots. Amer. J. Botany 29: 813-815.
8. DiIorio, A. A., R. D. Cheetham, and P. J. Weathers. 1992. Growth of transformed roots in a nutrient mist bioreactor: Reactor performance and evaluation. Applied Microbiology and Biotech. 37: 457-462.
9. Flores, H. E. 1987. Use of plant cells and organ culture in the production of biological chemicals. In Biotechnology in Agricultural Chemistry, 67-85. Washington, D.C.: ACS Symposium Series.
10. Flores, H. E., and P. Filner. 1985. Metabolic relationships of putrescine, GABA, and alkaloids in cell and root cultures of Solanaceae. In Primary and Secondary Metabolism in Plant Cell Cultures, 174-186. K. H. Neumann and E. Reinhard, eds. Heidelbeig, Germany: Springer-Verlag.
11. Flores, H. E., and W. R. Curtis. 1991. Approaches to understanding and manipulating the biosynthetic potential of plant roots. Proc. N.Y. Acad. Science 655: 188-209.
12. Flores, H. E., M. W. Hoy, and J. J. Pickard. 1987. Secondary metabolites from root cultures. Trends in Biotech. 5: 64-69.
13. Flores, H. E., Y. R. Dai, J. L. Cuello, I. E. Maldonado-Mendoza, and V. M. Loyola-Vargas. 1993. Green roots: Photosynthesis and photoautotrophy in an underground plant organ. Plant Physiology 101: 363-371.
14. Fogler, H. S. 1986. Elements of Chemical Reaction Engineering. Englewood Cliffs, N.J.: Prentice-Hall.
15. Furze, J. M., M. J. C. Rhodes, A. J. Parr, R. J. Robins, I. M. Whitehead, and D. R. Threlfall. 1991. Abiotic factors elicit sesquiterpenoid phytoalexin production but not alkaloid production in transformed root cultures of Datura stramonium. Plant Cell Reports 10: 111-114.
16. Gamborg, O. L., R. A. Miller, and K. Ojima. 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Research 50: 151-158.
17. Hamill, J. D., A. J. Parr, R. J. Robins, and M. J. C. Rhodes. 1986. Secondary product formation by cultures of Beta vulgaris and Nicotiana rustica transformed by Agrobacterium rhizogenes. Plant Cell Reports 5: 111-114.
18. Hamill, J. D., A. J. Parr, M. Rhodes, R. J. Robins, and N. J. Walton. 1987. New routes to plant secondary products. Bio/Technology 5: 800-804.
19. Hilton, M. G., and M. J. C. Rhodes. 1990. Growth and hyoscyamine production of "hairy root" cultures of Datura stramonium in a modified stirred tank reactor. Applied Microbiology and Biotech. 33: 132-138.
20. Jaziri, M. M. Legros, J. Homes and M. VanHaelen. 1988. Tropine alkaloids production by "hairy root" cultures of Datura stramonium and Hyoscyamus niger. Phytochemistry 27: 419-420.
21. Jung, G., and D. Tepfer. 1987. Use of genetic transformation by the Ri T-DNA of Agrobacterium rhizogenes to stimulate biomass and tropane alkaloid production in Atropa belladonna and Calystegia sepium roots grown in vitro. Plant Science 50: 145-151.
22. Kamada, H., N. Okamura, M. Satake, H. Harada, and K. Shimomura. 1986. Alkaloid production by "hairy root" cultures in Atropa belladonna. Plant Cell Reports 5: 239-242.
23. Kino-Oka, M., Y. Hongo, M. Taya, and S. Tone. 1992. Culture of red beet "hairy" roots in bioreactor and recovery of pigment released from the cells by repeated treatment of oxygen starvation. J. Chem. Eng. of Japan 25(5): 490-495.
24. Kondo, O., H. Honda, M. Taya, and T. Kobayashi. 1989. Comparison of growth properties of carrot "hairy" root in various bioreactors. Applied Microbiology and Biotech. 32: 291-294.
25. Levenspiel, O. 1972. Chemical Reaction Engineering. 2nd ed. New York, N.Y.: John Wiley and Sons.
26. Mahagamasekara, M. G. P., and P. Doran. 1997. Intergeneric co-culture of genetically transformed organs for the production of scopolamine. Phytochemistry 47(1): 17-25.
27. Mano, Y., S. Nabeshima, C. Matsui, and H. Ohkawa. 1986. Production of tropane alkaloids by "hairy root" cultures of Scopolia japonica. Agric. Biol. Chem. 50: 1715-1722.
28. McKinley, T. C. 1993. Polyacetylene biosynthesis in root culture. MS thesis. University Park, Pa.: Pennsylvania State University.
29. Nichol, D. 1994. An Introduction to Genetic Engineering. Cambridge, U.K.: Cambridge University Press.
30. Pareilleux, A. 1988. The large-scale cultivation of plant cells. In Plant Cell Biotechnology, 18: 314-328. M. S. S. Pais, F. Mavituna, and J. M. Novais, eds. Berlin, Germany: Springer-Verlag.
31. Parr, A. J., and J. D. Hamill. 1987. Relationship between Agrobacterium rhizogenes transformed "hairy" roots and intact uninfected Nicotiana plants. Phytochemistry 26: 3214-3245.
32. Payne, G. F., M. L. Shuler, and P. Brodelius. 1987. Large-scale plant cell culture. In Large-Scale Cell Culture Technology, 195-229. B. K. Lydersen, ed. New York, N.Y.: Hansers Publishers.
33. Ramakrishnan, D., and W. R. Curtis. 1994. Fluid dynamic studies on plant root cultures for application to bioreactor design. In Advances in Plant Biotechnology: Production of Secondary Metabolites, 281-305. S. Furusaki and D. Ryu, eds. Amsterdam, The Netherlands: Elsevier Science Publishers.
34. Rhodes, M. C. J., M. Hilton, A. J. Parr, J. D. Hamill, and R. J. Robins. 1986. Nicotine production by "hairy root" cultures of Nicotiana rustica: Fermentation and product recovery. Biotech. Letters 8(6): 415-420.
35. Rowan, D. E., and W. R. Curtis. 1991. Correlation of root growth to oxygen transfer. Unpublished data. University Park, Pa.: Pennsylvania State University, Department of Chemical Engineering.
36. Sahai, O. M. 1994. Plant tissue culture. In Bioprocess Production of Flavor, Fragrance, and Color Ingredients, 239-275. A. Gabelman, ed. New York, N.Y.: John Wiley and Sons.
37. Salisbury, F. B., and C. W. Ross. 1985. Plant Physiology. 3rd ed. Belmont, Cal.: Wadsworth Publishing.
38. Sasson, A. 1992. Production of useful biochemicals by higher-plant cell cultures: Biotechnological and economic aspects. In Biotechnology: Economic and Social Aspects, 81-109. E. J. Da Silva, C. Ratledge, and A. Sasson, eds. Cambridge, U.K.: Cambridge University Press.
39. Scragg, A. 1991. Plant cell bioreactors. In Plant Cell and Tissue Culture, 220-239. A. Stafford and G. Warren, eds. Maidenhead, Berkshire, U.K.: Open University Press.
40. Shimomura, K., M. Satake, and H. Kamada. 1986. Production of useful secondary metabolites by "hairy" roots transformed with Ri-plasmid. In Proc. 6th International Congress of Plant Tissue and Cell Culture. D. Somers, B. G. Gagenbach, D. D. Beisboer, W. P. Hackett, and C. E. Green, eds. Minneapolis, Minn.: University of Minnesota Press.
41. Shimomura, K., H. Sudo, H. Saga, and H. Kamada. 1991. Shikonin production and secretion by "hairy root" cultures of Lithospermum erythrorhizon. Plant Cell Reports 10: 282-285.
42. Shuler, M., and F. Kargi. 1992. Bioprocess Engineering: Basic Concepts. Upper Saddle River, N.J.: Prentice Hall.
43. Singh, G., and W. R. Curtis. 1994. Reactor design for plant root cultures. In Biotechnological Applications of Plant Cultures, 185-206. P. D. Shargool and T. T. Ngo, eds. Boca Raton, Fla.: CRC Press.
44. Singh, G., G. R. Reddy, and W. R. Curtis. 1994. Use of binding measurements to predict elicitor dosage requirements for secondary metabolite production from "root" cultures. Biotech. Progress 10(4): 365-371.
45. Subroto, M. A., and P. Doran. 1994. Production of steroidal alkaloids by "hairy" roots of Solanum aviculare and the effect of gibberellic acid. Plant Cell, Tissue, and Organ Culture 38: 93-102.
46. Taiz, L., and E. Zeiger. 1991. Plant Physiology. Redwood City, Cal.: Benjamin/Cummings Publishing.
47. Taya, M., A. Yoyama, O. Kondo, and T. Kobayashi. 1989. Growth characteristics of plant "hairy" roots and their cultures in bioreactors. J. Chem. Eng. of Japan 22(1):84-89.
48. Tepfer, D. 1984. Transformation of several species of higher plants by Agrobacterium rhizogenes: Sexual transmission of the transformed genotype and phenotype. Cell 37: 959-967.
49. Toivonen, L., M. Ojala, and V. Kauppinen. 1990. Indole alkaloid production by "hairy" root cultures of Catharanthus roseus: Growth kinetics and fermentation. Biotech. Letters 12(7): 519-524.
50. Yoshikawa, T., and T. Furuya. 1987. Saponin production by cultures of Panax ginseng transformed with Agrocbacterium rhizogenes. Plant Cell Reports 6: 449-453.