Analytical and preparative native polyacrylamide gel electrophoresis: Investigation of the recombinant and natural major grass pollen allergen Phl p 2

Electrophoresis

Volumn 25, Issue 1, 2004, Pages 14-19

  Suck, Roland ^a   Petersen, Arnd ^b   Weber, Bernhard ^a   Becker, Wolf Meinhard ^b   Fiebig, Helmut ^a   Cromwell, Oliver ^a  

^a ALLERGOPHARMA JOACHIM GANZER KG   (Germany)

^b RESEARCH CENTER BORSTEL   (Germany)

Author keywords

Immunoglobulin E binding; Isoform; Native polyacrylamide gel electrophoresis; Phl p 2; Recombinant allergen

Indexed keywords

ALLERGENS; BINDING ENERGY; ELECTROPHORESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; QUALITY CONTROL; SODIUM DODECYL SULFATE;

IMMUNOGLOBULIN E; IMMUNOGLOBULIN E-BINDING; ISOFORMS; NATIVE POLYACRYLAMIDE GEL ELECTROPHORESIS; PHL P 2; POLLEN ALLERGEN; POLYACRYLAMIDE GEL ELECTROPHORESIS; RECOMBINANT ALLERGEN; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; WESTERN BLOTS;

SULFUR COMPOUNDS;

IMMUNOGLOBULIN E; ISOPROTEIN; POLLEN ANTIGEN; RECOMBINANT ANTIGEN;

AMINO TERMINAL SEQUENCE; ANALYTIC METHOD; ANTIGEN BINDING; ANTIGEN STRUCTURE; ARTICLE; CONTROLLED STUDY; ELECTRODIALYSIS; GRASS POLLEN; IMMUNOREACTIVITY; ISOLATION PROCEDURE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DEGRADATION; QUALITY CONTROL; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SEPARATION TECHNIQUE; WESTERN BLOTTING;

EID: 1042280324     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.200305697     Document Type: Article

References (18)

1. Valenta, R., Kraft, D., Curr. Opin. Immunol. 2002, 14, 718-727.
2. Harwanegg, C., Laffer, S., Hiller, R., Müller, M. W., Kraft, D., Spitzauer, S., Valenta, R., Clin. Exp. Allergy 2003, 33, 7-13.
3. Andersson, K., Lidholm, J., Int. Arch. Allergy Immunol. 2003, 130, 87-107.
4. Petersen, A., Bufe, A., Schlaak, M., Becker, W.-M., Int. Arch. Allergy Immunol. 1995, 108, 49-54.
5. Brodard, V., David, B., Görg, A., Peltre, G., Int. Arch. Allergy Immunol. 1993, 102, 72-80.
6. Suck, R., Hagen, S., Cromwell, O., Fiebig, H., J. Immunol. Methods 1999, 229, 73-80.
7. Levy, D., Davies, J., O'Hehir, R., Suphioglu, C., Electrophoresis 2001, 22, 1900-1905.
8. Dolecek, C., Vrtala, S., Laffer, S., Steinberger, P., Kraft, D., Scheiner, O., Valenta, R., FEBS 1993, 335, 299-304.
9. Petersen, A., Bufe, A., Schramm, G., Schlaak, M., Becker, W.-M., Int. Arch. Allergy Immunol. 1995, 108, 55-59.
10. Niederberger, V., Hayek, B., Vrtala, S., Laffer, S., Twardosz, A., Vangelista, L., Sperr, W. R., Valent, P., Rumpold, H., Kraft, D., Ehrenberger, K., Valenta, R., Spitzauer, S., FASEB J. 1999, 13, 843-856.
11. Suck, R., Weber, B., Schäffer, B., Diedrich, E., Kamionka, T., Fiebig, H., Cromwell, O., J. Chromatogr. B 2003, 787, 357-368.
12. Laemmli, U. K., Nature 1970, 227, 680-685.
13. European Pharmacopeia Supplement 2000, 2.2.31.
14. Suck, R., Weber, B., Kahlert, H., Hagen, S., Cromwell, O., Fiebig, H., Int. Arch. Allergy Immunol. 2000, 121, 284-291.
15. Weber, B., Slamal, H., Suck, R., J. Biochem. Biophys. Methods 2003, 56, 219-232.
16. Kahlert, H., Petersen, A., Becker, W. M., Schlaak, M., Mol. Immunol. 1992, 29, 1191-1201.
17. Ansari, A. A., Shenbagamurthi, R, Marsh, D. G., J. Biol. Chem. 1989, 264, 11181-11185.
18. Ansari, A. A., Shenbagamurthi, P., Marsh, D. G., Biochemistry 1989, 28, 8665-8670.