Production of 6-aminopenicillanic acid in aqueous two-phase systems by recombinant Escherichia coli with intracellular penicillin acylase

Biotechnology Letters

Volumn 26, Issue 2, 2004, Pages 97-101

  Xue Jun, Cao ^a   Xing Yan, Wu ^a   Fonseca, Luis J P ^b   Cabral, Joaquim M S ^b   Marcos, João Carlos ^c  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b INSTITUTO SUPERIOR TÉCNICO   (Portugal)

^c UNIVERSITY OF MINHO   (Portugal)

Author keywords

6 aminopenicillanic acid production; Aqueous two phase systems; Bioconversion; Penicillin acylase; Recombinant E. Coli A56

Indexed keywords

6 AMINOPENICILLANIC ACID; DRUG DERIVATIVE; PENICILLANIC ACID; PENICILLIN AMIDASE; PENICILLIN G; RECOMBINANT PROTEIN;

ARTICLE; CHEMISTRY; CRYSTALLIZATION; ESCHERICHIA COLI; GENETICS; METABOLISM; PH; TEMPERATURE;

CRYSTALLIZATION; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; PENICILLANIC ACID; PENICILLIN AMIDASE; PENICILLIN G; RECOMBINANT PROTEINS; TEMPERATURE;

ESCHERICHIA COLI;

EID: 1042268335     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:BILE.0000012885.62462.f3     Document Type: Article

References (9)

1. Albertsson PÅ (1986) Partition of Cell Particles and Macromolecules, 3rd edn. New York: Wiley-Interscience.
2. Andersson E, Mattiasson B, Barbel HH (1984) Enzymatic conversion in aqueous two phase systems: deacylation of benzyl penicillin to 6-aminopenicillanic acid with penicillin acylase. Enzyme Microb. Technol. 6: 301-306.
3. Balasinghan K, Warburton D, Dunnill P, Lilly MD (1972) The isolation and kinetics of penicillin amidase from Escherichia coli. Biochim. Biophys. Acta 276: 250-256.
4. Chen JH, Xu LD (1991) Analytical Methods of Antibiotics in Industry. Beijing: Medicinal Scientific publisher of China.
5. Ma SH, Du JY, Qu TM, Shi GD, Yu XI, Wu RP, Feng YM, Yang SL (1992) Production of 6-APA penicillanic acid in cell membrane bioreactor. Chinese J. Biotechnol. 8: 71-81.
6. Pessina A, Luthi P, Luisi PL (1988) Amide-bond syntheses catalyzed by penicillin acylase. Helv. Chem. Acta 71: 631-641.
7. Stambolieva N, Mincheva Z, Galunsky B, Kalcheva V (1992) Penicillin amidase-catalysed transfer of low specific acyl moiety. Synthesis of 7-benzoxazolonylacetamido desacetoxycephalosporanic acid. Enzyme Microb. Technol. 14: 496-501.
8. Xuejun C, Liu Y, Wu XY (1993) Determination of polyethylene glycol in aqueous two-phase systems. Fenxi Huaxue 21: 1470.
9. Xuejun C, Wu X (1994) Studies on the assay method of dextran concentration in ATPS proceeding bioconversion with penicillin acylase. Chinese J. Antibiotics 19: 61-62.