EPR spin trapping of a radical intermediate in the urate oxidase reaction

Nucleosides, Nucleotides and Nucleic Acids

Volumn 23, Issue 8-9, 2004, Pages 1131-1134

  Busi, E ^a   Terzuoli, L ^a   Basosi, R ^a   Porcelli, B ^a   Marinello, E ^a  

^a UNIVERSITY OF SIENA   (Italy)

Author keywords

Oxidation; Radical intermediate; Spin trap; Uric acid; Uricase

Indexed keywords

5 DIETHOXYPHOSPHORYL 5 METHYL 1 PYRROLINE 1 OXIDE; ALLANTOIN; RADICAL; URATE OXIDASE; URIC ACID;

CATALYSIS; CHEMICAL REACTION; CONFERENCE PAPER; ELECTRON SPIN RESONANCE; ENZYME MECHANISM; FENTON REACTION; NONHUMAN; OXIDATION REDUCTION REACTION; OXIDATIVE STRESS; PURINE METABOLISM; REACTION ANALYSIS; SIMULATION; SPIN TRAPPING;

ANIMALS; BACILLUS; CANDIDA; CATALYSIS; CYCLIC N-OXIDES; ELECTRON SPIN RESONANCE SPECTROSCOPY; FREE RADICALS; HUMANS; HYDROGEN PEROXIDE; HYDROXYL RADICAL; OXYGEN; SPIN LABELS; SPIN TRAPPING; SWINE; URATE OXIDASE; URIC ACID;

BACILLUS FASTIDIOSUS; CANDIDA; SUIDAE;

EID: 10344225660     PISSN: 15257770     EISSN: None     Source Type: Journal    
DOI: 10.1081/NCN-200027390     Document Type: Conference Paper

References (5)

1. Wu, X.; Lee, C.C.; Muzny, D.M.; Caskey, C.T. Urate oxidase: primary structure and evolutionary implications. Proc. Natl. Acad. Sci. 1989, 86, 9412-9416.
2. Moriwaki, Y.; Yamamoto, T.; Higashino, K. Enzymes involved in purine metabolism - a review of histochemical localization and functional implication. Histol. Histopathol. 1999, 14, 1321-1340.
3. Pitts, O.M.; Priest, D.G. Uriease reaction intermediate. Mechanism of borate and hydroxide ion catalysis. Biochemistry 1973, 12, 1358-1363.
4. Kahn, K.K.; Tipton, P.A. Spectroscopic characterization of intermediates in the urate oxidase reaction. Biochemistry 1998, 37, 11651-11659.
5. Frejaville, C.; Karoui, H.; Tuccio, B.; Le Moine, F.; Culcasi, M.; Pietri, S.; Lauricella, R.; Tordo, P. 5-(Diethoxyphosphoryl)-5-methyl-1- pyrroline N-oxide: a new efficient phosphorylated nitrose for the in vitro and in vivo spin trapping of oxygen-centred radicals. J. Med. Chem. 1995, 38, 258-265.