Aqueous sol-gel encapsulation of genetically engineered Moraxella spp. cells for the detection of organophosphates

Biosensors and Bioelectronics

Volumn 20, Issue 7, 2005, Pages 1433-1437

  Yu, Diana ^a   Volponi, Joanne ^a   Chhabra, Swapnil ^a   Brinker, C Jeffrey ^b   Mulchandani, Ashok ^c   Singh, Anup K ^a  

^a SANDIA NATIONAL LABORATORIES   (United States)

^b SANDIA NATIONAL LABORATORIES   (United States)

^c University of California Riverside   (United States)

Author keywords

Acetylcholine esterase; Biosensors; Moraxella; Organophosphate hydrolase; Organophosphates; Sol gel

Indexed keywords

BACTERIA; BIOSENSORS; ENCAPSULATION; ENZYME IMMOBILIZATION; ENZYME KINETICS; GENETIC ENGINEERING; PH; POROUS MATERIALS; REACTION KINETICS; SILICATES; SOL-GELS;

SODIUM SILICATE; SOL-GEL ENCAPSULATION;

CELLS;

ORGANOPHOSPHATE; SILICON DIOXIDE;

AQUEOUS SOLUTION; ARTICLE; CELL ENCAPSULATION; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME STABILITY; ENZYMIC BIOSENSOR; GEL; GENETIC ENGINEERING; MORAXELLA; PH MEASUREMENT; POROSITY; PROTEIN HYDROLYSIS; REDUCTION; SURFACE PROPERTY;

BIOSENSING TECHNIQUES; HYDROGEN-ION CONCENTRATION; MORAXELLA; ORGANISMS, GENETICALLY MODIFIED; PHOSPHORIC ACID ESTERS;

BACTERIA (MICROORGANISMS);

EID: 10044275041     PISSN: 09565663     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bios.2004.04.022     Document Type: Article

References (18)

1. Al-Saraj M., Abdel-Latif M., El-Nahal I., Baraka R. Bioaccumulation of some hazardous metals by sol-gel entrapped microorganisms. J. Non-Cryst. Solids. 248:1999;137-140
2. Bhatia R., Brinker C., Gupta A., Singh A. Aqueous sol-gel process for protein encapsulation. Chem. Mat. 12:2000;2434-2441
3. Coiffier A., Coradin T., Roux C., Bouvet O., Livage J. Sol-gel encapsulation of bacteria: a comparison between alkoxide and aqueous routes. J. Mat. Chem. 11:2001;2039-2044
4. Dunn B., Miller J., Dave B., Valentine J., Zink J. Strategies for encapsulating biomolecules in sol-gel matrices. Acta Mater. 46:1998;737-741
5. Fennouh S., Guyon S., Jourdat C., Livage J., Roux C. Encapsulation of bacteria in silica gels. CR Acad. Sci. II C. 2:1999;625-630
6. Fennouh S., Guyon S., Livage J., Roux C. Sol-gel entrapment of Escherichia coli. J. Sol-Gel Sci. Technol. 19:2000;647-649
7. Gill I. Bio-doped nanocomposite polymers: sol-gel bioencapsulates. Chem. Mater. 13:2001;3404-3421
8. Lan E., Dunn B., Valentine J., Zink J. Encapsulation of the ferritin protein in sol-gel derived silica glasses. J. Sol-Gel Sci. Technol. 7:1996;109-116
9. Lan E., Dunn B., Zink J. Sol-gel encapsulated anti-trinitrotoluene antibodies in immunoassays for TNT. Chem. Mat. 12:2000;1874-1878
10. Livage J., Coradin T., Roux C. Encapsulation of biomolecules in silica gels. J. Phys.: Condens. Mat. 13:2001;R673-R691
11. Mulchandani A., Mulchandani P., Kaneva I., Chen W. Biosensor for direct determination of organophosphate nerve agents using recombinant Escherichia coli with surface-expressed organophosphorus hydrolase. 1. Potentiometric microbial electrode. Anal. Chem. 70:1998;4140-4145
12. Mulchandani A., Shengtian P., Chen W. Fiber-optic enzyme biosensor for direct determination of organophosphate nerve agents. Biotechnol. Prog. 15:1999;130-134
13. Mulchandani P., Mulchandani A., Chen W. Flow injection amperometric enzyme biosensor for direct determination of organophosphate nerve agents. Environ. Sci. 35:2001;2562-2565
14. Shimazu M., Mulchandani A., Chen W. Simultaneous degradation of organophosphorus pesticides and p-nitrophenol by a genetically engineered moraxella spp. with surface-expressed organophosphorus hydrolase. Biotechnol. Bioeng. 76:2001;318-324
15. Singh A.K., Flounders A.W., Volponi J.V., Ashley C.S., Wally K., Schoeniger J.S. Development of sensors for direct detection of organophosphates. Part I: immobilization, characterization and stabilization of acetylcholinesterase and organophosphate hydrolase on silica supports. Biosens. Bioelectron. 14:1999;703-713
16. Tuovinen K., Kalistekorphonen E., Raushel F., Hanninen O. Phosphotriesterase - a promising candidate for use in detoxification of organophosphates. Fundam. Appl. Toxicol. 23:1994;578-584
17. Whitaker, J., 1972. Principles of Enzymology for the Food Sciences. Marcel Dekker Inc., New York.
18. Yang L., Saavedra S. Chemical sensing using sol-gel derived planar wave-guides and indicator phases. Anal. Chem. 67:1995;1307-1314