Proton donor in yeast pyruvate kinase: Chemical and kinetic properties of the active site Thr 298 to cys mutant

Biochemistry

Volumn 43, Issue 48, 2004, Pages 15230-15245

  Susan Resiga, Delia ^a   Nowak, Thomas ^a  

^a UNIVERSITY OF NOTRE DAME   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; CONFORMATIONS; FLUORESCENCE; ISOTOPES; PERTURBATION TECHNIQUES; PH EFFECTS; PHOSPHATES; PURIFICATION; REACTION KINETICS; SOLVENTS; YEAST;

FRUCTOSE 1,6-BIPHOSPHATE (FBP); HILL COEFFICIENTS; MUTATIONS; YEAST PYRUVATE KINASE (YPK);

ENZYMES;

CYSTEINE; FRUCTOSE 1,6 BISPHOSPHATE; MAGNESIUM; PROTON; PYRUVATE KINASE; THIOL GROUP; THREONINE;

ARTICLE; CATALYSIS; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME INACTIVATION; ENZYME KINETICS; ENZYME LOCALIZATION; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; FLUORESCENCE ANALYSIS; METAL BINDING; MUTAGENESIS; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; YEAST;

ADENOSINE DIPHOSPHATE; BINDING SITES; CATALYSIS; CIRCULAR DICHROISM; CYSTEINE; DEUTERIUM EXCHANGE MEASUREMENT; ENZYME INHIBITORS; FRUCTOSEDIPHOSPHATES; HYDROGEN-ION CONCENTRATION; IODOACETATES; KINETICS; LIGANDS; MAGNESIUM; MANGANESE; MUTAGENESIS, SITE-DIRECTED; PHOSPHOENOLPYRUVATE; PROTEIN BINDING; PROTONS; PYRUVATE KINASE; SACCHAROMYCES CEREVISIAE PROTEINS; SPECTROMETRY, FLUORESCENCE; THREONINE;

EID: 10044255244     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi049864d     Document Type: Article

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