Evaluation of MPN Method Combined with PCR Procedure for Detection and Enumeration of Vibrio parahaemolyticus in Seafood

Journal of the Food Hygienic Society of Japan

Volumn 44, Issue 6, 2003, Pages 289-293

  Miwa, Norinaga ^a   Nishio, Tomohiro ^a   Arita, Yono ^a   Kawamori, Fumihiko ^a   Masuda, Takashi ^a   Akiyama, Masato ^a  

^a SHIZUOKA INSTITUTE OF ENVIRONMENT AND HYGIENE   (Japan)

Author keywords

MPN; PCR; Seafood; Vibrio parahaemolyticus

Indexed keywords

AGAR; CITRIC ACID; HEMOLYSIN; PEPTONE; SUCROSE; THIOSULFATE; WATER;

ARTICLE; BACTERIAL GENE; BACTERIUM COLONY; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; BILE; CONTROLLED STUDY; CULTURE MEDIUM; FOOD CONTAMINATION; HEAT SENSITIVITY; INTERMETHOD COMPARISON; NONHUMAN; POLYMERASE CHAIN REACTION; RELIABILITY; SAMPLE; SEA FOOD; SPECIES DIFFERENCE; TECHNIQUE; VIBRIO ALGINOLYTICUS; VIBRIO PARAHAEMOLYTICUS; WORK;

BACTERIA (MICROORGANISMS); VIBRIO ALGINOLYTICUS; VIBRIO PARAHAEMOLYTICUS;

EID: 0942279803     PISSN: 00156426     EISSN: None     Source Type: Journal    
DOI: 10.3358/shokueishi.44.289     Document Type: Article

References (11)

1. Food and Drug Administration, Bacteriological Analytical Manual, 8th ed. Chapter 9. AOAC International, Gaithersburg, USA (1998).
2. Janda, J. M., Powers, C., Bryant, R. G., Abbott, S. L., Current perspectives on the epidemiology and pathogenesis of clinically significant Vibrio spp. Clin. Microbiol. Rev., 1, 245-267 (1988).
3. Sakazaki, R., Nakanishi, H., Control of food poisoning with Vibrio parahaemolyticus. Shokuhin Eisei Kenkyu (Food Sanitation Res.), 27(6), 21-26 (1977).
4. Picard, C., Ponsonnet, C., Paget, E., Nesme, X., Simonet, P., Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction. Appl. Environ. Microbiol., 58, 2,717-2,722 (1992).
5. Mantynen, V., Niemela, S., Kaijalainen, S., Pirhonen, T., Lindstrom, K., MPN-PCR-quantification method for staphylococcal enterotoxin c1 gene from fresh cheese. Int. J. Food Microbiol., 36, 135-143 (1997).
6. Miwa, N., Nishina, T., Kubo, S., Atsumi, M., Most probable number method combined with nested polymerase chain reaction for detection and enumeration of enterotoxigenic Clostridium perfringens in intestinal contents of cattle, pig and chicken. J. Vet. Med. Sci., 59, 89-92 (1997).
7. Alam, M. J., Tomochika, K., Miyoshi, S., Shinoda, S., Environmental investigation of potentially pathogenic Vibrio parahaemolyticus in the Seto-Island Sea, Japan. FEMS Microbiol. Lett., 208, 83-87 (2002).
8. Taniguchi, H., Ohta, H., Ogawa, M., Mizuguchi, Y., Cloning and expression in Escherichia coli of Vibrio parahaemolyticus thermostable direct hemolysin and thermolabile hemolysin genes. J. Bacteriol., 162, 510-515 (1985).
9. Bej, A. K., Patterson, D. P., Brasher, C. W., Vickery, M. C. L., Jones, D. D., Kaysner, C. A., Detection of total and hemolysin-producing Vibrio parahaemolyticus in shellfish using multiplex PCR amplification of tl, tdh and trh. J. Microbiol. Methods, 36, 215-225 (1999).
10. Hara-Kudo, Y., Nishina, T., Nakagawa, H., Konuma, H., Hasegawa, J., Kumagai, S., Improved method for detection of Vibrio parahaemolyticus in seafood. Appl. Environ. Microbiol., 67, 5,819-5,823 (2001).
11. Hara-Kudo, Y., Sugiyama, K., Nishina, T., Saitoh, A., Nakagawa, H., Ichihara, T., Konuma, H., Hasegawa, J., Kumagai, S., Detection of TDH-producing Vibrio parahaemolyticus 03: K6 from naturally contaminated shellfish using an immunomagnetic separation method and a chromogenic agar medium. Kansensyougaku Zasshi (Jpn. J. Infect. Dis.), 75, 955-960 (2001).