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Volumn 126, Issue 4, 2004, Pages 1088-1095

Influences of Ribonucleotide on a Duplex Conformation and Its Thermal Stability: Study with the Chimeric RNA-DNA Strands

Author keywords

[No Author keywords available]

Indexed keywords

BIOTECHNOLOGY; CONFORMATIONS; DNA; STEREOCHEMISTRY; THERMODYNAMIC STABILITY;

EID: 0942276311     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja037314h     Document Type: Article
Times cited : (59)

References (43)
  • 27
    • 0017666766 scopus 로고
    • Because the CD spectra reported for uridine and thymine are almost identical [Sprecher, C. A.; Johnson, W. C., Jr. Biopolymers 1977, 16, 2243.] it is unlikely that the substitution of U with T changed the CD spectra.
    • (1977) Biopolymers , vol.16 , pp. 2243
    • Sprecher, C.A.1    Johnson Jr., W.C.2
  • 30
    • 0942273788 scopus 로고    scopus 로고
    • note
    • It is recognized that estimation of the nucleotide concentration includes an ∼10% uncertainly. The experimental uncertainty affects the peak intensity while it does not change the shape of the spectra. This would cause a weak correlation between the peak intensity and the stability. To eliminate the uncertainty in the concentration, we used the ratio of the peak intensity at 265 nm with that at 240 nm as shown in Figure 3B, A plot of the ratio of the peak intensity at 270 nm with that at 240 nm also showed a linear fit with a linear-correlation coefficient (r) of 0.89 (data not shown).
  • 31
    • 0033565267 scopus 로고    scopus 로고
    • 37s at 1 M NaCl with those at 100 mM NaCl [Nakano, S.; Hara, H.; Sugimoto, N. Nucleic Acids Res. 1999, 27, 2957.], the relationship may also be observed at lower ionic conditions.
    • (1999) Nucleic Acids Res. , vol.27 , pp. 2957
    • Nakano, S.1    Hara, H.2    Sugimoto, N.3
  • 33
    • 0942306227 scopus 로고    scopus 로고
    • note
    • 1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.