Enhancement of Apparent Substrate Selectivity of Proteinase K Encapsulated in Liposomes Through a Cholate-Induced Alteration of the Bilayer Permeability

Biotechnology and Bioengineering

Volumn 85, Issue 2, 2004, Pages 222-233

  Yoshimoto, Makoto ^a   Wang, Shaoqing ^a   Fukunaga, Kimitoshi ^a   Treyer, Mike ^b   Walde, Peter ^b   Kuboi, Ryoichi ^c   Nakao, Katsumi ^a  

^a YAMAGUCHI UNIVERSITY   (Japan)

^b ETH ZURICH   (Switzerland)

^c OSAKA UNIVERSITY   (Japan)

Author keywords

Apparent enzyme activity; Apparent substrate selectivity; Cholate modulated liposomes; Enzyme leakage; Membrane permeability; Proteinase K containing liposomes

Indexed keywords

BIOLOGICAL MEMBRANES; BIOREACTORS; BIOTECHNOLOGY; ENCAPSULATION; HYDROLYSIS; LIPIDS; MECHANICAL PERMEABILITY; SUBSTRATES;

LIPOSOMES; MEMBRANE PERMEABILITY;

ENZYMES;

2 OLEOYL 1 PALMITOYLPHOSPHATIDYLCHOLINE; ANILIDE; CASEIN; CHOLIC ACID; LIPOSOME; PROPIONIC ACID DERIVATIVE; PROTEINASE K;

AQUEOUS SOLUTION; ARTICLE; BILAYER MEMBRANE; ENCAPSULATION; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; HYDROLYSIS; MEMBRANE PERMEABILITY; MICELLE;

CHOLATES; COATED MATERIALS, BIOCOMPATIBLE; DIFFUSION; ENDOPEPTIDASE K; ENZYME ACTIVATION; LIPID BILAYERS; LIPOSOMES; PERMEABILITY; PHOSPHATIDYLCHOLINES; SUBSTRATE SPECIFICITY;

EID: 0942276125     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10891     Document Type: Article

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