2D and 3D Spatially Addressed Arrays for High-Throughput Automated Synthesis of Combinatorial Libraries

Journal of Combinatorial Chemistry

Volumn 6, Issue 1, 2004, Pages 43-49

  Patek, Marcel ^a   Safar, Pavel ^a   Smrčina, Martin ^a   Wegrzyniak, Eric ^a   Bjergarde, Kirsten ^a   Weichsel, Aleksandra ^a   Strop, Peter ^a  

^a Selectide Combinatorial T   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMIDE; AMINO ACID; AROMATIC CARBOXYLIC ACID; HYDROXYL GROUP; POLYMER; POLYPROPYLENE; REAGENT; SOLVENT;

ALKYLATION; ARTICLE; AUTOMATION; CARBON NUCLEAR MAGNETIC RESONANCE; CHEMICAL REACTION; COMBINATORIAL CHEMISTRY; DENSITY; EVAPORATION; INFRARED SPECTROSCOPY; MITSUNOBU REACTION; PROTON NUCLEAR MAGNETIC RESONANCE; PURIFICATION; QUANTITATIVE ANALYSIS; REACTOR; ROOM TEMPERATURE; SOLID; SYNTHESIS; TECHNIQUE; TEMPERATURE;

EID: 0942268100     PISSN: 15204766     EISSN: None     Source Type: Journal    
DOI: 10.1021/cc0300311     Document Type: Article

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28. The position of frames within the stack is not important at this step.
29. The relative location of frames within the stack is now important for final compound tracking.
30. Depending on the presence of moisture as well as on the nature of the amino acid R1{1-32}, partial hydrolysis of the carboxamides 7 to the corresponding acids was observed.
31. The remaining 4% of the expected compounds was not detected by LC/MS analysis. Spatial address was thus successfully maintained and correctly mapped into final 96 well plates. These results therefore validate the presented concept for the synthesis of larger combinatorial libraries.
32. These results are in accordance with the intended purity profile and correlate closely with performance of R1-R2-R3 building blocks during the chemistry development. Such a purity profile reflects the presence of desired building blocks while it fully meets our internal purity standards for combinatorial libraries (75% of the library compounds to have purities greater than 50%, multiple products are separable on HPLC, and side products are reproducibly formed).
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