Production of secreted hydrolytic enzymes by continuous high-cell-density cultivation of Colpidium campylum

European Journal of Protistology

Volumn 39, Issue 4, 2003, Pages 455-460

  Scheidgen Kleyboldt, G ^a   Kuchta, K ^a   Kiy, T ^a   Tiedtke, A ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Bioreactor; Biotechnology; Colpidium; Enzyme production; Fermentation; Lysosomal enzymes

Indexed keywords

CILIOPHORA; COLPIDIUM; COLPIDIUM CAMPYLUM; PROTOZOA; VISCUM; VISCUM ALBUM;

EID: 0842323823     PISSN: 09324739     EISSN: None     Source Type: Journal    
DOI: 10.1078/0932-4739-00020     Document Type: Article

References (23)

1. Banno Y., Sasaki N. and Nozawa Y. (1987): Secretion heterogeneity of lysosomal enzymes in Tetrahymena pyriformis. Exp. Cell Res. 170, 259-268.
2. Dive D. and Rasmussen L. (1978): Growth studies on Colpidium campylum under axenic conditions. J. Protozool. 25, 42A.
3. Florin-Christensen J., Florin-Christensen M., Tiedtke A. and Rasmussen L. (1990): The role of secreted acid hydrolases in the utilization of complex nutrients by Tetrahymena. Microb. Ecol. 19, 311-316.
4. Foissner W., Blatterer H., Berger H. and Kohmann F. (1991): Taxonomische und ökologische Revision der Ciliaten des Saprobiensystems-Band I: Cyrtophorida, Oligotrichida, Hypotrichida, Colpodea. Informationsberichte des Bayer. Landesamtes für Wasserwirtschaft, vol 1/91, 478 pp.
5. Gosselin Y., Lognay G. and Thonart, P. (1989): Improvement of fed batch mass culture for γ-linolenic biosyntheses by Tetrahymena rostrata (protozoa). Biotechnol. Lett. 11, 423-426.
6. Keenan K., Erlich E., Donnelly K., Basel M., Hutner R., Kassoff R. and Crawford A. (1978): Particle-based axenic media for tetrahymenoids. J. Protozool. 25, 385-387.
7. Kidder G., Dewey V. and Fuller R. (1954): Nitrogen Requirements of Glaucoma scintillans and Colpidium campylum. Proc. Soc. Exp. Biol. Med. 86, 685-689.
8. Kiy T. and Brinkmann K. (2002): Obtainment of γ-linolenic acid from protozoa of the genus Colpidium. US Patent 6,403,345 B1.
9. Kiy T., Scheidgen-Kleyboldt G. and Tiedtke A. (1996): Production of lysosomal enzymes by continuous high-cell-density fermentation of the ciliated protozoon Tetrahymena thermophila in a perfused bioreactor. Enz. Microbiol. Technol. 18, 268-274.
10. Kiy T. and Tiedtke A. (1991): Lysosomal enzymes produced by immobilized Tetrahymena thermophila. Appl. Microbiol. Biotechnol. 35, 14-18.
11. Kiy T. and Tiedtke A. (1992a): Continuous high-cell-density fermentation of the ciliated protozoon Tetrahymena in a perfused bioreactor. Appl. Microbiol. Biotechnol. 38, 141-146.
12. Kiy T. and Tiedtke A. (1992b): Mass cultivation of Tetrahymena thermophila yielding high cell densities and short generation times. Appl. Microbiol. Biotechnol. 37, 76-579.
13. Kiy T., Vosskühler C., Rasmussen L. and Tiedtke A. (1993): Three pools of lysosomal enzymes in Tetrahymena thermophila Exp. Cell Res. 205, 286-292.
14. Laughlin T., Henry J., Phares E., Long M. and Olins D. (1983): Methods for the large-scale cultivation of an Oxytricha (Ciliophora: Hypotricha). J. Protozool. 30, 63-64.
15. Müller M. (1972): Secretion of acid hydrolases and its intracellular source in Tetrahymena pyriformis. J. Cell Biol. 52, 478-487.
16. Munro I. (1985): Protozoa as sources of commercially produced enzymes - a review. Process Biochem. 20, 139-144.
17. Murricane C. (1986): Purification of a proteinase and a proteinase inhibitor from Tetrahymena pyriformis. Int. J. Biochem. 18, 403-406.
18. Proper G. and Graver J. (1966): Mass culture of the protozoa Colpoda steinii. Biotechnol. Bioeng. 8, 287-296.
19. Rasmussen L. and Kludt T. (1970): Particulate material as a prerequisite for rapid cell multiplication in Tetrahymena cultures. Exp. Cell. Res. 59, 457-463.
20. Schönefeld U., Alfermann A. and Schultz J. (1986): Economic mass cultivation of Paramecium tetraurelia on a 200-liter scale. J. Protozool. 33, 222-225.
21. Sudo R., Kobayashi K. and Aiba S. (1975): Some experiments and analysis of a predator-prey model: interaction between Colpidium campylum and Alcaligenes faecalis in continuous and mixed culture. Biotechnol. Bioeng. 17, 167-184.
22. Thiele J., Honer-Schmid O., Wahl J., Kleefeld G. and Schultz J. (1980): A new method for axenic mass cultivation of Paramecium tetraurelia. J. Protozool. 27, 118-121.
23. Tiedtke A. (2001): Biotechnology with Protozoa. In: Rehm H.-J. and Reed G. (eds): Biotechnology, Volume 10, pp. 137-149. Wiley-VCH, Weinheim, Federal Republic of Germany.